- Pk:1 KIT
- Description:STEMdiff™ branching lung organoid maturation kit
Serum-free media for maturation of branching lung organoids from human ES and iPS cells.
Mature and maintain human branching lung organoids in long-term (>28 days) culture using STEMdiff™ branching lung organoid maturation kit. Serum-free STEMdiff™ branching lung organoid maturation kit may be used to reproducibly mature human embryonic stem (ES) and induced pluripotent stem (iPS) cell-derived lung bud organoids generated using STEMdiff™ branching lung organoid kit. Resulting organoids will develop proximal and distal-like branching airway epithelial structures expressing EPCAM, NKX2.1, SOX2, SOX9, MUC1, and P63. When maintained beyond 28 days in a Matrigel® sandwich, there is an increase in expression levels of markers for more mature lung cells, such as SFTPC, SFTPB, and ABCA3.