Experimentation kits, mitochondrial DNA analysis using PCR

The mitochondria are thought to have evolved from a symbiotic relationship between prokaryotic and eukaryotic cells. Mitochondria have their own DNA and are only inherited via the maternal line. In this experiment, students amplify two regions of their mitochondrial DNA.

• For 25 students
• Set up 30 minutes; PCR 2 hours or overnight; electrophoresis 45 minutes

Also required: Micropipettes (5 to 50 µl) with tips, water bath, thermal cycler, electrophoresis tank and power supply.

Delivery information: Kit includes instructions, proteinase K, PCR beads, control DNA and primers, microtubes, chelating agent, agarose, DNA ladder, practice gel loading solution, gel loading dye, electrophoresis buffer, and gel stain.