In this experiment, students see a blue colour change in transformed cells due to the switching on of a gene. The pGAL plasmid gives a blue colour dye due to the production of the β-galactosidase protein by the lacZ gene. IPTG is not required in this experiment since pGAL contains the complete lacZ gene.

• For 10 lab groups
• Complete in 50 minutes and grow overnight

Also required: 37 °C incubator, water bath.