Peqlab peqGOLD, Protein Marker I (Unstained)
27-1010
:
- Pk:1 KIT
- Description:Protein Marker I (unstained, ready to use), 2×1000 µl, sufficient for 400 mini gels or 200 standard gels
- Environmentally Preferable:
peqGOLD Protein Marker I is a mixture of seven purified proteins which are re-dissolved 'ready to use' in loading buffer. The proteins resolve into sharp, tight bands in the range of 14,4 to 116,0 kDa when analysed by SDS-PAGE and stained with Coomassie Brilliant Blue R-250.
Mini gel application: 5 to 10 µl/well
Standard gel application: 10 to 20 µl/well
: peqGOLD Protein Marker I is optimised for runs on 12% SDS polyacrylamide gels. 8 to 10% gels may cause proteins with low molecular weights to migrate with the dye front. On 12 to 15% and gradient gels all bands are visible.
The marker is optimised for use with Coomassie Brlliant Blue R-250, but can also be used with other gel staining methods (e.g. silver staining). As this method is 10 to 100 times more sensitive than Coomassie Blue staining, the amount of marker applied should be decreased accordingly.
peqGOLD Protein Marker I contains 2% SDS and is, therefore, not recommended to be used in native polyacrylamide gels for determining native molecular weights of proteins.
The marker is optimised for use with Coomassie Brlliant Blue R-250, but can also be used with other gel staining methods (e.g. silver staining). As this method is 10 to 100 times more sensitive than Coomassie Blue staining, the amount of marker applied should be decreased accordingly.
peqGOLD Protein Marker I contains 2% SDS and is, therefore, not recommended to be used in native polyacrylamide gels for determining native molecular weights of proteins.