Specifications

• Pk:
  100 µG
• Seq:
  02
• Protein/peptide name:
  Di-Ubiquitin (K48-linked)
• Purity:
  >95% (SDS-PAGE)
• Formulation:
  Liquid, containing 50 mM TRIS pH 7,5, 1 mM EDTA and 5 mM DTT.
• Purification:
  Ubiquitin mutants [K48C] and [D77] were expressed in BL21 (λDE3) E. coli. Proteins were purified by cation exchange chromatography. Di-ubiquitin was synthesised by linking [K48C] ubiquitin and [D77] ubiquitin in the presence of ubiquitin conjugating enzyme E2-25K (UbcH1) (Prod. No. BML-UW9020) and human ubiquitin activating enzyme E1 (Prod. No. BML-UW9410). The product was purified by ion exchange chromatography. Purity was confirmed by SDS-PAGE and protein concentration estimated by absorbance at 280 nm.