- Pk:10 µG
- Protein/peptide type:Recombinant
- Source:E. coli
- Species:Human
- Tag sequence:GST tag
- Storage conditions:Stable for at least 6 months after receipt when stored at −20 °C.
- Biological activity:Maintains pluripotency of mouse and human embryonic stem cells. The LIF (GST-tagged) from AdipoGen has the same activity as cleaved LIF.
- Protein synonyms:Leukemia Inhibitory Factor|Melanoma-derived LPL Inhibitor|Differentiation-stimulating Factor|D Factor
- Protein/peptide name:LIF
- Purity:>95% (SDS-PAGE)
- Sequence:23-202
- Formulation:Lyophilized, Contains PBS.
LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities also include the induction of hematopoietic differentiation in normal and myeloid leukaemia cells, the induction of neuronal cell differentiation and the stimulation of acute-phase protein synthesis in hepatocytes. LIF activates JAK & STAT signalling in human embryonic stem (ES) cells, but this pathway does not maintain pluripotency in these cells, which instead rely on FGF2-mediated ERK signalling. By contrast, mouse ES cells can be maintained by LIF-mediated JAK & STAT signalling. LIF binds to a high affinity heterodimeric receptor complex consisting of two proteins: LIF-R alpha that binds LIF with low affinity and the 130kDa (gp130) subunit that by itself does not bind LIF, but is required for high affinity binding of LIF.
Fusion-Tag: GST tag
This recombinant proteins is for research use only.