These columns have the same coated chiral selector as found in the normal phase stationary phases, however in comparison, they use a different bounded silica as the base material which stabilises the CSP.

Reversed phase analytical columns were developed specifically for aqueous-organic mobile phases. They are suited for applications where the sample is presented in aqueous media (e.g. biological samples) or for samples that require flexibility in term of pH range.

Extreme pH values must be avoided as they can damage the silica gel used in these columns. These columns are also frequently used in LC/MS applications.