OneCup it‘s so easy! The OneCup assay comes along with a minimum of pipetting steps. Everything is included in the mix. You only need to combine 20μl of the mix with 2 μl of your DNA sample and everything is ready. You have a minimum of hands-on time and you avoid mistakes during pipetting. You can run the test on all common block cyclers. Application: Detection of Listeria monocytogenes in food samples and environmental samples subsequent to a pre-enrichment step according to DIN EN ISO 20837 and 20838.Test principle. The test is dedicated to detect DNA of Listeria monocytogenes in a sample using Real-Time PCR in compliance with the ISO method mentioned below. The kit uses the TaqMan® principle. Thus the requirements of the standard, that an amplification product has to be confirmed by a hybridization step, is fulfilled. Each reactions contains an internal amplification control. Therefore, false negative results due to inhibition of the reaction can be excluded. The test included the UNG enzyme to prevent re-amplification of contaminating amplicons.

• All components are in one cup
• Just add 2 µl sample to 20 µl mix
• TaqMan probes
• Contains internal amplification control in HEX channel
• Taget in FAM channel
• Contains UNG to protect from re-amplification
• Positive DNA and negative control included