Specifications

• Source:
  E. coli
• Species:
  Rat
• Storage conditions:
  Store at 4 °C for up to two weeks. For long term storage, aliquot and store at −20 °C, avoid freeze/thaw cycles.
• Biological activity:
  The ED50 range as determined by the dose-dependant proliferation of BALB/3T3 cells was found to be less than 0.2 ng/ml, corresponding to specific activity of 5,000,000 IU/mg.
• Protein synonyms:
  HBGH-2
• Protein/peptide name:
  FGF basic
• Purity:
  > 97.0% as determined by RP-HPLC and analysis by SDS-PAGE
• Solubility:
  It is recommended to reconstitute the lyophilized Rat bFGF in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Sequence:
  PALPEDGGGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHV KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTEE CFFFERLESN NYNTYRSRKY SSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS.
• Formulation:
  FGF-b was lyophilized from mg/ml solution after extensive dialysis against 20 mM phosphate buffer, pH 7.4 and 130mM NaCl.
• Tested applications:
  SDS-PAGE , HPLC , FuncS