Immobilisation kit, AminoLink™ Plus, Pierce™

AminoLink™ Plus immobilisation kit contains AminoLink™ Plus coupling resin and additional reagents to perform five protein immobilisations, with expected yields of 2 to 40 mg protein per column. The coupling resin uses aldehyde-activated agarose beads for high-yield covalent coupling of antibodies (proteins) via primary amines to prepare columns for affinity purification.

Aldehyde-activated crosslinked 4% beaded agarose
Immobilise molecules via primary amines (−NH₂)
Efficient coupling over a wide range of pH (4 to 10) and buffer conditions (PBS or other non-amine buffer with or without organic solvent)
Coupling reaction results in stable, leak-resistant secondary amine bond between resin and ligand

: Kit contains AminoLink™ Plus columns, 2 ml (5 columns), PBS pack (makes 500 ml), citrate-carbonate buffer pack (makes 500 ml), sodium cyanoborohydride solution, 5 M (0,5 ml), quenching buffer (60 ml), wash solution (240 ml), and column accessories.