DyLight™ Fluor phosphine reagents are phosphine-activated fluorescent dyes for specific labeling and detection of azide-tagged molecules, which enables use of fluorescence imaging in metabolic labeling strategies.

• Easily dissolves in water-miscible solvents (eg. DMSO) for subsequent dilution in aqueous reaction mixtures with cell lysates and other biological samples
• Reaction chemistry occurs effectively in simple buffer conditions requiring no accessory reagents such as copper or reducing agents, and does not interfere with fluorescence applications
• The phosphine reactive group is specific in biological samples for bioorthogonal azide-tagged molecules, ensuring that fluorescent labeling is specific
• DyLight™ 488, 550 and 650 are intense, highly stable fluorophores for green, orange and red fluorescent detection

When used in combination with azide labeling strategies, phosphine-activated dyes enable selective fluorescent labeling for detection of protein interactions and post-translational modifications using fluorescence imaging technologies. The phosphine group conjugates to azide groups by the Staudinger reaction mechanism.