HyClone™ AdvanceSTEM™ cell culture media are optimised for growth of embryonic stem cell cultures.

• ES cell-qualified – no prescreening required
• Mycoplasma free and sterility tested
• DMEM4SC is formulated without L-glutamine or sodium pyruvate and is ideal for use with 129 ES cells
• IMDM4SC contains no L-glutamine and supports excellent growth of B6 ES cells.

Pluripotent mESCs have a very distinct morphology when cultured under these conditions; growing as tightly clustered colonies with smooth phase bright borders. When co-cultured in ES cell media with a mouse embryonic fibroblast (MEF) feeder layer, these stem cell cultures are suitable for electroporation, expansion, or further experimentation.

Providing optimal culture conditions can be one of the greatest challenges in stem cell research, particularly in keeping cells in an undifferentiated state or directing differentiation when desired. HyClone AdvanceSTEM line of stem cell culture products can help you meet those challenges. Classic culture conditions for 129-derived murine embryonic stem cells (mESCs) include culturing in medium containing DMEM, FBS, LIF and co-culturing with primary mouse embryonic fibroblasts (MEFs).