Native endoproteinase Glu-C from Staphylococcus aureus. Serine protease that specifically hydrolyses peptide bonds at the carboxylic side of glutamic acid and aspartic acid residues. Inhibited by DFP and α2-macroglobulin. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.