Affinity purification media, GammaBind™ Plus Sepharose™

GammaBind™ Plus Sepharose™ is GammaBind G, type 3, covalently immobilised to Sepharose™ CL-6B by malimide linkage.

For affinity chromatography
Recombinant Protein G type 3 coupled to Sepharose CL-6B for enhanced binding to rat and mouse IgG subclasses at laboratory scale
Binds to all IgG subclasses from human, mouse, and rat; binds total IgG from guinea pig, rabbit, goat, cow, sheep, and horse
Maleimide activation coupling method
Yields up to 18 mg/mL mouse IgG
Stable in all commonly used aqueous buffers

GammaBind G, Type 3, a recombinant form of streptococcal protein G, binds to the Fc region of IgG from a variety of mammalian species. GammaBind Plus Sepharose may be used to analyze and purify classes, subclasses and fragments of immunoglobulins from any biological fluid or cell culture medium. Since only the Fc region is involved in binding, the Fab region is still available for binding antigen.

pH range:
3 - 9
Matrix:
Cross-linked agarose, 6%
Ligand:
Recombinant GammaBind G type 3 lacking albumin-binding region
Storage temperature:
2 - 8 °C
Binding capacity:
> 35 mg human IgG/mL
Flow rate:
<130 cm/h