Supplier: SIGMA ALDRICH MICROSCOPY

Glycerol alters the hygroscopic property of gelatin and its permeability to water vapour. Glycerol gelatin or glycerine jelly medium is an aqueous slide mounting media, used for mounting samples in microscopy and histology.

Supplier: SIGMA ALDRICH MICROSCOPY

PolyFreeze is a water-soluble support matrix and a form of embedding medium for frozen sectioning. PolyFreeze sections flawlessly at routine frozen specimen thickness of 5-6 μ and leaves no slide residue. It provides for -20 ºC cryostat sectioning in rapid set embedment. This medium freezes quickly supporting the tissue for sectioning at 3 mm and up with no cracking of the matrix at temperatures from -8 °C to -25 °C.

Supplier: VWR Chemicals

Decalcifier according to Kristenson.

Supplier: Avantor

Mounting medium for coverslipping of slides.

Supplier: VWR Chemicals

HistoChoice® clearing agent is a much less hazardous replacement for xylene/toluene.

Supplier: Avantor

Mounting medium for coverslipping of slides.

Supplier: VWR Chemicals

High viscosity mounting medium for cryotomy.

Supplier: SIGMA ALDRICH MICROSCOPY

Immersion Oil has a defined refractive index of about 1,516. It is important that the refractive index (nD) is about 1,5, similar to that of glass. This enables a homogeneous oil immersion to be achieved.

Supplier: SIGMA ALDRICH MICROSCOPY

Suitable for use with large and small mineralized (undecalcified) bone sections. Yields clear, hard blocks for cutting sections. Not water-soluble.

Supplier: Avantor

Formalin (dissolved formaldehyde) is used in histopathology laboratories.

Supplier: VWR Chemicals

Contains a mixture of hydrochloric acid and alcohols with some EDTA.

Supplier: VWR Chemicals

High viscosity mounting media for cryotomy. Less viscous than O.C.T.

Supplier: VWR Chemicals

Acidified alcoholic formalin for the fixation of glycogen.

Supplier: VWR Collection

Immersion oil is used to increase microscope resolution.

Supplier: Merck

Hard tissue like bones or teeth, and all specimens of calcified tissue like bone marrow punches require special pre-treatment before routine processing by a histological laboratory.

Supplier: VWR Chemicals

When sectioning frozen tissue for rapid surgical biopsy diagnosis or for research (typically in Histochemistry), the tissue sample must be firmly attached to the microtome stage or chuck. Embedding material such as OCT are preferred as these also provide a supportive and protective aid to sectioning.

Supplier: SIGMA ALDRICH MICROSCOPY

The Epoxy Embedding Medium Kit is a very widely used embedding medium for electron microscopy. The embedding formulation originally published by Luft (1961) is excellent for both plant and animal tissues. Due to the low viscosity of the resin, it penetrates the tissue specimen faster than Araldite and other polymers. It hardens easily and uniformly at low temperatures with the addition of dodecenylsuccinic anhydride (DDSA), methyl nadic anhydride (MNA), and the accelerator 2,4,6-tris(dimethylaminomethyl)phenol (DMP-30). Slight shrinkage may occur during curing. This kit is useful for embedding a variety of tissues as a wide range of hardness can be obtained with this resin to suit a specific tissue type by using two different anhydride curing agents (DDSA and MNA).

Supplier: VWR Chemicals

Glue for improving adherance of histologicals sections to slides. Alternative to albumin-glycerol.

Supplier: VWR Chemicals

Cytology fixative specially designed for the conservation and transport of cytology specimens.

Supplier: VWR Chemicals

Specially used for freezing of surgical parts before the cutting process with cryostat or with a freezing microtome.

Supplier: VWR Chemicals

Fixing solution with 0,02% formaldehyde and 0,01% citric acid in aqueous solution.

Supplier: VWR Chemicals

A range of mounting media for manual and automatic mounting of slides. The Q Path® Coverquick range consists of four different mounting media with different viscosities to meet all microscopy needs. The number after the name indicates increasing viscosity: Q Path® Coverquick 1000 has the lower viscosity (around 260 mPaS) and Q Path® Coverquick 4000 has the higher viscosity (around 920 mPaS).

Supplier: VWR Chemicals

Fixative, combining the coagulant qualities of alcohol, to enable a better fixation of nuclear and cytoplasmic elements. The erythrocytes are lysed and, therefore, do not obscure observation of the elements of interest. This fixative can be used according to the Saccomanno method.

Supplier: Merck

Entellan® new is a new mounting medium for cover slippers and is specially suited for commercial automated mounting instruments that operate with cover slippers. Its viscosity ranges between 250 and 600 mPas, 1,00869 has a small viscosity range. This narrow viscosity range improves and accelerates the application, as neither the dropping rate nor the volume need to be reset after the first run with a new bottle of Entellan® new.

Supplier: VWR Chemicals

VWR® Bouin tissue fixative solution.

Supplier: SIGMA ALDRICH MICROSCOPY

CC/mount is an aqueous based permanent mounting medium with a very high refractive index, which when applied to the stained tissue sections can store the tissue specimens permanently without the fading of the chromogens. Because of the superior refractive index of CC/mount, tissues mounted in this medium look like specimens mounted with organic based mounting media. No coverslipping is required with CC/mount. However, if coverslipping is required, dried CC/mount can be post mounted by using an organic based mounting medium. CC/mount permits the long-term storage of antigens localised using organic insoluble chromogen substrates, including AEC, DAB, Fast Red, BCIP/NBT, BCIP/INT and fluorescent dyes like FITC and phycobiliproteins. The high pH of CC/mount ensures increased stability of fluorescence intensity.

Supplier: VWR Chemicals

Safemount Q Path® is a mounting medium, supplied in an PET bottle, which is compatible with Safesolv. It glues the coverglass to the slide and fixes the probe in position after staining.

Supplier: VWR Chemicals

Fixing solution with 0,02% formaldehyde in methanol 50% (v/v)).

Supplier: VWR Chemicals

DC1 slow acting decalcifiers contain a mixture of formic acid and formalin.

Supplier: VWR Chemicals

This medium-acting decalcifier contains a mixture of hydrochloric acids.