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Western Blotting Reagents

Nucleic acids or proteins in a complex sample can be visualized using electrophoresis and blotting procedures and the compatible reagents. Improving the overall efficiency of electrophoresis systems, these reagents accommodate a variety of blotting techniques and with antibodies provided against tens of thousands of different proteins. These methods are used in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.

Western blotting detection reagents, ECL western blotting substrate

Western blotting detection reagents, ECL western blotting substrate

Supplier: Thermo Fisher Scientific

ECL™ Western blotting substrate is an entry-level Western blotting substrate.

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Western blot assay kits, ScanLater™

Western blot assay kits, ScanLater™

Supplier: Molecular Devices

The ScanLater™ Western Blot Assay Kits are a time-resolved fluorescence (TRF)-based assay, optimal for quantitating as little as femtogram protein samples.

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Western blotting kit, ECL substrate, Pierce™

Western blotting kit, ECL substrate, Pierce™

Supplier: Thermo Fisher Scientific

The Fast Western Blot Kit, ECL Substrate contains optimised reagents that shorten the time which is required to perform a typical chemiluminescent Western blot from 4 hours down to approximately 55 minutes.

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Western blot stripping buffer, restore PLUS™

Western blot stripping buffer, restore PLUS™

Supplier: Thermo Fisher Scientific

Some antibodies are difficult to remove from Western blots and require Longer incubation times or incubation temperatures greater than 22 °C. Restore™ PLUS Western Blot Stripping Buffer was developed to reduce incubation times while keeping incubations at room temperature. High-affinity antibodies can be quickly and effectively stripped from Western blots without removing transferred proteins, thereby allowing multiple reprobes of the target.

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Luminata™ Chemiluminescent ELISA HRP Substrates

Luminata™ Chemiluminescent ELISA HRP Substrates

Supplier: Merck

Chemiluminescent HRP detection reagent for use on immunoblots, Western blotting, dot/slot blotting.

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Immobilon™ Block Noise Canceling Reagents

Immobilon™ Block Noise Canceling Reagents

Supplier: Merck

Immobilon® Block Noise cancelling reagents are designed especially for Western blotting applications. This family of protein-free, ready-to-use buffers has been optimised to reduce background levels when using chemiluminescence, fluorescent, or phosphotyrosine detection.

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Peroxidase Indicator Reagent

Supplier: SIGMA ALDRICH MICROSCOPY

It contains p-Phenylenediamine diHCL (1 part) and catechol (2 parts). Commonly referred to as Hanker Yates reagent. Kit component in the Sigma Leukocyte peroxidase (myeloperoxidase) Procedure, 390A.

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VWR® Fixing Solution for Isoelectric Focusing in Polyacrylamide Gel, Reag. Ph. Eur. 1138700

VWR® Fixing Solution for Isoelectric Focusing in Polyacrylamide Gel, Reag. Ph. Eur. 1138700

Supplier: VWR Chemicals

VWR®, Fixing solution for isoelectric focusing in polyacrylamide ge

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Luminata™ Western HRP Substrates Samples for Antibodies of All Affinities

Luminata™ Western HRP Substrates Samples for Antibodies of All Affinities

Supplier: Merck

Western blotting, binding assays, amino acid analysis, N-terminal protein sequencing, dot/slot blotting, glycoprotein visualisation, lipopolysaccharide analysis.

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Chemicon® ReBlot Plus Kits

Chemicon® ReBlot Plus Kits

Supplier: Merck

Western blotting is a commonly used technique for studying protein function and localisation. Typically, protein samples are electrophoresed on SDS-page and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of southern and northern blots, it has been difficult to reuse western blots.

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Western blots, pre-made, mouse subcellular fraction specific blot

Western blots, pre-made, mouse subcellular fraction specific blot

Supplier: G-Biosciences

The subcellular fractions from liver tissue are prepared by carefully dissecting out anatomically and functionally distinct regions of adult mouse livers.

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Chemiluminescent detection reagents, Luminata™ Western HRP substrates

Chemiluminescent detection reagents, Luminata™ Western HRP substrates

Supplier: Merck

Luminata™ Western HRP substrates are a family of premixed, ready to use chemiluminescent reagents for the detection of HRP-based Westerns.

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Immunoblotting kits, MaxTag™

Immunoblotting kits, MaxTag™

Supplier: Rockland Immunochemicals

These accurate and sensitive kits are ideal for Western blotting or dot blotting methods. They offer flexibility with two unique substrates: Bright Blue TMB substrate and Stable Brown DAB substrate. Components are colour-coded for error-free results. With MaxTag™ you can run up to 40 (10×10cm) immunoblots.

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Nitrotyrosine BSA for immunoblotting experiments

Nitrotyrosine BSA for immunoblotting experiments

Supplier: Cayman Chemical

This Nitrotyrosine BSA can be used as a positive control for immunoblotting experiments using Nitrotyrosine Polyclonal Antibody and Nitrotyrosine Monoclonal Antibody.

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Diluent for western blotting, Swift™ Western Diluent

Diluent for western blotting, Swift™ Western Diluent

Supplier: G-Biosciences

The Swift™ Western Diluent is a solution that reduces the blocking and antibody incubations on Western blot membranes to <60 minutes. Swift™ Western Diluent generates comparable results to traditional Western blotting procedures and other commercial “fast” Western blotting kits.

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Chemiluminescent detection reagents, Immobilon® Western HRP substrates

Chemiluminescent detection reagents, Immobilon® Western HRP substrates

Supplier: Merck

Immobilon® Western HRP substrates are compatible with both PVDF and nitrocellulose membranes, as well as all commonly used buffers and blocking reagents.

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Chemicon® ReBlot Plus Strong Antibody Stripping Solution, 10X

Chemicon® ReBlot Plus Strong Antibody Stripping Solution, 10X

Supplier: Merck

Western blotting is a commonly used technique for studying protein function and localisation. Typically, protein samples are electro-phoresed on SDS-page and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of southern and northern blots, it has been difficult to reuse western blots.

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Peqlab peqGOLD AceGlow™, Blotting Reagent, Chemiluminescence Substrate

Peqlab peqGOLD AceGlow™, Blotting Reagent, Chemiluminescence Substrate

Supplier: VWR Peqlab

AceGlow™ Chemiluminescence Substrate is for the horseradish peroxidase-mediated detection of proteins and nucleic acids. There are two variants: AceGlow™ Essential for standard blots with a detection sensitivity of 1 picogram and a signal emission for up to 8 hours, and AceGlow™ with highest sensitivity down to 10 femtogram and amplified, long lasting light signal emission over 24 hours.

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CYTAG™ CGH labelling kits

CYTAG™ CGH labelling kits

Supplier: ENZO LIFE SCIENCES

The CYTAG™ CGH labeling kit produces high quality data for comparative genomic hybridisation (aCGH) using as little as 0,25 µg of genomic DNA, without a need for pre-amplification.

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IHC chromogen substrate (AEC, stable), HIGHDEF®

Supplier: ENZO LIFE SCIENCES

HIGHDEF® red IHC chromogen can be used in conjunction with peroxidase-based immunostaining systems.

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Normal cell line blots, human

Normal cell line blots, human

Supplier: G-Biosciences

Ready to screen, normal tissue human cell line blots from epithelial, endothelial, skin, and muscle tissue.

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picoLUCENT™ PLUS-HRP Kit

picoLUCENT™ PLUS-HRP Kit

Supplier: G-Biosciences

The picoLUCENT™ PLUS-HRP kit is based on an ultra sensitive luminol substrate that produces chemiluminescence upon reaction with horseradish peroxidase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films.

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Kidney tissue region blot, single species

Kidney tissue region blot, single species

Supplier: G-Biosciences

These are ready to screen, normal tissue, kidney tissue region blots from mouse and rat. The kidney tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult mouse and rat kidney.

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Cell extract HL60 (non-induced)

Supplier: ENZO LIFE SCIENCES

Whole-cell extract of human HL-60 leukemia cells.

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Immunodetection reagents, femtoLUCENT™ PLUS

Immunodetection reagents, femtoLUCENT™ PLUS

Supplier: G-Biosciences

The femtoLUCENT™ PLUS Reagent Kit facilitates the quick and sensitive non-isotopic detection of proteins on transfer membranes.

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picoLUCENT™ PLUS-AP kit

picoLUCENT™ PLUS-AP kit

Supplier: G-Biosciences

The picoLUCENT™ PLUS-HRP kit is based on an ultra sensitive luminol substrate that produces chemiluminescence upon reaction with alkaline phosphatase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films.

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Human cancer cell line blot

Human cancer cell line blot

Supplier: G-Biosciences

Ready to screen human cancer cell line blots.

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Eye tissue region blot, single species

Eye tissue region blot, single species

Supplier: G-Biosciences

These are ready to screen, normal tissue, eye tissue region blots from rat and rabbit. The eye tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult rat and rabbit eyes.

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Chemiluminescent acridinium esters

Chemiluminescent acridinium esters

Supplier: ENZO LIFE SCIENCES

Chemiluminescent acridinium esters are commonly used to label antibodies, proteins, nucleic acids, and some peptides, and are frequently used as detection reagents in immunoassays.

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Single tissue blots, multiple species

Single tissue blots, multiple species

Supplier: G-Biosciences

Single tissue and multiple species blot contains four protein lanes with an identical GenLysate™ tissue lysate from human, mouse and rat species, and a protein marker lane.

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