You searched for: Electrophoresis Reagents

Supplier: PanReac AppliChem

The main applications for prestained protein markers are the monitoring of protein migration during SDS-PAGE, verification of Western transfer efficiency on membranes, and estimation of protein sizes on SDS-polyacrylamide gels and Western blots.

Supplier: LONZA

Thse DNA ladders provides rapid and easy size determination of the DNA samples. Each band has an ideal 50 % G-C content for precise sizing and sharp resolution. It is free from contaminating plasmid DNA with minimal background.

Supplier: LONZA

ProSieve™ Color protein marker have been labelled with three different fluorescent dyes. These markers are supplied as a lyophilised solid containing the buffer salts and detergent found in the typical Laemmli buffer system. It contains nine proteins with approximate masses of 10, 15, 20, 25, 40, 50, 80, 125, and 190 kDa.

Supplier: Merck Millipore (Novagen)

The Perfect Protein™ Western Markers consist of seven recombinant S•Tag™/His•Tag® fusion proteins that can be detected on any Western blot using an S-protein AP or HRP conjugate. As an additional option, the markers can also be detected with an His•Tag Monoclonal Antibody and an AP or HRP conjugated secondary antibody. The Perfect Protein Western Markers serve as precise size standards that appear simultaneously on the blot with target proteins, eliminating the uncertainty and imprecision associated with other methods.

Supplier: Biotium

A visible three-colour protein marker for SDS-PAGE or western blotting, with 10 bands ranging from 10 to 180 kDa.

Supplier: Cytiva

Each kit contains the detection reagent, HRP conjugated antibodies, and blocking agent sufficient for detection of 1000 cm² membrane.

Supplier: Cytiva

Amersham ECL detection reagent uses enhanced luminol-based detection suitable for all routine confirmatory Western blotting experiments.

Supplier: Cytiva

CyDye™ Mono-Reactive Maleimide Fluorescent Dyes are intensely fluorescent and highly water soluble, providing significant advantages over other existing fluorophores.

Supplier: MP Biomedicals

This antibody diluent is compatible with common stabilising compounds at pH 7,3.

Supplier: Thermo Fisher Scientific

DNA ladders are used in gel electrophoresis to determine the size and quantity of testing DNA fragments of genomic, plasmid, and PCR DNA. They are commonly used in the studies of plasmid DNA and PCR products.

Supplier: Thermo Fisher Scientific

From 250 to 5000 base pairs: 5 bands from 125 to 1000 bp; four bands from 1500 to 5000 bp. Recommended load volume 20 µl.

Supplier: Merck Millipore (Novagen)

PCR Molecular Size Marker contains a mixture of eight defined DNA fragments ranging from 50 to 2000 bp at convenient size intervals for characterising small DNA products. The markers are supplied in gel loading buffer containing two tracking dyes that do not interfere with UV illumination of ethidium bromide-stained bands. A separate vial of 6X Loading Buffer is included. Each vial of markers is sufficient for 50 lanes on TBE or TAE agarose stained with ethidium bromide. The recommended amount for loading (5 µl) produces bands of even intensity that are bright, sharp and easy to photograph.

Supplier: Cytiva

Amersham ECL Prime Blocking Reagent is blocking Amersham Protran NC and Amersham Hybond PVDF membranes used with Amersham ECL start, Amersham ECL, Amersham, ECL Prime, Amersham ECL Select and Amersham ECL Plex.

Supplier: Cytiva

ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 minute chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

Supplier: Cytiva

Amersham ECL Plex™ Western blotting combination packs offer a complete system for fluorescent Western blotting, with improved formulation Cy3 conjugates for Lower background.

Supplier: Cytiva

Enhanced chemiluminescence (ECL) detection reagents for reliable detection of medium and high expression proteins.

Supplier: Cytiva

Cy™ fluorescent amidites for incorporation of Cy3, Cy3,5, Cy5, Cy5,5 fluorescent dyes at any position in an oligonucleotide during synthesis.

Supplier: Cytiva

Chemiluminescence detection reagent for detection of high to medium abundant proteins.

Supplier: Cytiva

pI markers are lyophilized mixtures of stable, salt-free, highly purified proteins.

Supplier: Cytiva

For determination of molecular weight of uncharacterised protein samples with precision by comparison with carefully selected standards.

Supplier: Cytiva

Rainbow™ molecular weight markers enable faster and simpler identification of proteins on SDS polyacrylamide gels. These ready to load markers provide sharper, more intense bands on gels and blots, and have improved band spacing for more accurate molecular weight determination. The bright, distinctive colours of the markers allow easier confirmation of transfer to blotting membranes and orientation.

Supplier: Cytiva

Amersham™ ECL™ DualVue™ Western blotting markers perform two key functions. Firstly, the prestained marker proteins confirm protein transfer in addition to clearly defining blot orientation. Secondly, the recombinant tagged proteins are detected on film or by CCD imaging in conjunction with the target protein(s) of interest, enabling highly accurate molecular mass determination of the target protein(s). The tagged markers are detected easily and specifically by means of a specific conjugate that eliminates any cross contamination between target protein(s) and markers.

Supplier: Genscript

GenScript offers a wide selection of Protein molecular weight standards for SDS-PAGE and Western blot, including prestained, unstained, peptide, Western-viewable, and other standards.

Supplier: Cytiva

Amersham™ ECL™ Plex™ fluorescent Rainbow™ markers are protein molecular weight markers optimised for use with the ECL™ Plex™ Western blotting detection system. They are designed to provide visible marker bands on gels and membranes, as well as fluorescent images using Cy3 and Cy5 channels.

Supplier: Merck

SIGMAFAST™ BCIP/NBT (5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium) tablets have been developed for use in immunochemistry as a precipitating substrate for the detection of alkaline phosphatase activity.

Supplier: PerkinElmer

Liquid scintillation cocktail, Ultima Gold™, 2×5 l

Supplier: Serva

IEF carrier ampholyte, SERVALYT™, pH 6 - 7, analytical grade

Supplier: STEMCELL Technologies

DNA ladder consisting of 13 fragments from 250 to 10000 base pairs for determining the size of double-stranded DNA by gel electrophoresis.