You searched for: Electrophoresis Reagents

Supplier: G-Biosciences

Kingdom blot is prepared using total protein extracted from a variety of species, where whole species is not available the whole liver lysate is used. These blots can be used to identify similar proteins in other species, including human, chicken, frog, worm, Drosophila, yeast, Aspergillus, E. coli, Rhizobium and green algae.

Supplier: Rockland Immunochemicals

These Chemiluminescent Western Blot Kits allow for the detection of primary polyclonal or monoclonal antibodies provided by the user. After protein separation and transfer, the membrane is probed with primary antibody. Detection of the membrane-bound primary antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialised formulation of luminol, an extremely sensitive, non radioactive substrate that emits light and allows visualisation using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. Because of the extremely high sensitivity of the kit, primary and secondary antibodies can be used at greater dilutions. Primary antibodies currently diluted 1:1000 using an ECL™ substrate is the equivalent of a 1:5000 dilution using the FemtoMax™ substrate.

Supplier: Thermo Fisher Scientific

QuantaRed™ Enhanced Chemifluorescent HRP Substrate contains chemical enhancers to increase the fluorescent yield and sensitivity of 10-acetyl-3,7-dihydroxyphenoxazine (ADHP) chemifluorescence.

Supplier: EDVOTEK

20 µg for 20 gels.

Supplier: LONZA

Lonza SimplyLoad™ DNA ladders are ready to load directly onto the gel to maximise time and convenience. Each band has an ideal 50% G-C content for rapid, precise sizing and sharp resolution. Free from contaminating plasmid DNA so they have minimal background.

Supplier: Rockland Immunochemicals

TrueBlot® Western blot kits contain the critical supporting reagents, buffers, and substrates for immunoprecipitation and Western blotting of samples using TrueBlot® second step immunoblotting reagents in conjunction with the user's own primary IP antibody and primary (mouse, goat or rabbit IgG) Western blotting antibody.

Supplier: Thermo Fisher Scientific

PageRuler™ Plus pre-stained protein ladders are a mixture of nine blue-, orange- and green-stained proteins (10 to 250 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Supplier: Thermo Fisher Scientific

PageRuler™ pre-stained protein ladders are a mixture of ten blue-, orange- and green-stained proteins (10 to 180 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

Supplier: AAT BIOQUEST

ReadiUse™ 1 kb Plus DNA ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading.

Supplier: AAT BIOQUEST

The Gelite™ 100 bp to 1 kb DNA ladder is designed to help size and estimate the mass of double-stranded DNA and PCR products within the 100 to 10000 base pair range on agarose or polyacrylamide gels.

Supplier: Thermo Fisher Scientific

This wide range molecular weight marker contains 60 ng/μl of 21 fragments from 100 bp to 12000 bp. The bands appear in 100 bp increments from 100 to 1000 bp and in 1000 bp increments from 1 to 12 kb. Bands at 1 kb and 5 kb stain brighter to aid identification.

Supplier: Biotium

A visible three- colour protein marker for SDS-PAGE or western blotting, with 12 bands ranging from 8 to 245 kDa.

Supplier: Cytiva

Pharmalyte® carrier ampholytes, prepared by the co-polymerization of glycine, glycylglycine, amines and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals.

Supplier: Cytiva

Amersham™ ECL™ Prime is a highly sensitive Western blotting detection reagent characterised by stable signal emission, allowing for the possibility of repeated exposures and making it easier to process several blots in the same experimental run. Amersham™ ECL™ Prime delivers a detection system that is sensitive, stable, precisely quantitative across a wide dynamic range of protein levels, and conservative in its consumption of expensive antibody reagents. The high performance of Amersham™ ECL™ Prime is due to the presence of an enhancer in the reagent, increasing enzyme turnover, and thereby significantly increasing both signal intensity and duration. The light signal is further intensified by the addition of a catalyst.

Supplier: Cytiva

Amersham ECL Blocking Agent is for blocking nitrocellulose and PVDF membranes to be used with Amersham ECL, Amersham ECL Prime and Amersham ECL Select Western Blotting Detection Reagents

Supplier: G-Biosciences

DNAmark™ 1 kb Plus ladder contains 15 DNA bands ranging from 100 bp to 10 kb. The 500 bp and 3000 bp fragment are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 µl loading, all fragments except 500 bp and 3000 bp are 40 ng. The 500 bp and 3000 bp fragment are 100 ng.

Supplier: Merck Millipore (Novagen)

Molecular size markers for routine use in plasmid cloning, the Perfect DNA™ markers contain sets of DNA species having convenient, easy-to-remember sizes for agarose gel analysis. The markers have uniform band intensities except for the indicated reference bands, which are useful for instant band identification.

Supplier: LONZA

DNA marker is for rapid size estimation of PCR products and restriction fragments. DNA can be visualised by ethidium bromide staining or kinased with radiolabelled ³²P for detection by autoradiography.

Supplier: Cytiva

AlkPhos Direct™ Labelling and detection systems are based on the rapid, direct labelling of DNA or RNA probes with thermostable alkaline phosphatase. AlkPhos Direct™ combines the convenience of direct enzyme labelling (no blocking or antibody stages) with those of alkaline phosphatase detection (long light output and high sensitivity).

Supplier: Cytiva

ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

Supplier: Cytiva

Amersham ECL Select is a high sensitivity reagent for chemiluminescent Western blotting detection.

Supplier: Cytiva

CypHer5E is a red-excitable, pH-sensitive cyanine dye derivative that is minimally fluorescent at a basic pH and maximally fluorescent at an acidic pH.

Supplier: G-Biosciences

DNAmark™ 100 bp Plus ladder consists of 14 DNA fragments ranging in size from 100 to 3000 base pairs (bp). The 500 bp and 1200 bp fragment are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 µl loading, all fragments except 500 bp and 1200 bp are 40 ng. The 500 bp and 1200 bp fragment are 100 ng.

Supplier: G-Biosciences

DNAmark™ 100 bp ladder consists of 11 fragments ranging from 100 to 1500 base pairs (bp). The 500 bp fragment is present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 µl loading, all fragments except 500 bp are 40 ng. The 500 bp fragment is 100 ng.

Supplier: G-Biosciences

DNAmark™ 500 bp ladder consists of 10 DNA fragments ranging from 500 bp to 5000 bp. The 2000 bp fragment is present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 µl loading, all fragments except 2000 bp are 40 ng. The 2000 bp fragment is 100 ng.

Supplier: Thermo Fisher Scientific

DNA ladders are used in gel electrophoresis to determine the size and quantity of testing DNA fragments of genomic, plasmid, and PCR DNA. They are commonly used in the studies of plasmid DNA and PCR products.

Supplier: Thermo Fisher Scientific

From 250 to 5000 base pairs: 5 bands from 125 to 1000 bp; four bands from 1500 to 5000 bp. Recommended load volume 20 µl.

Supplier: Merck Millipore (Novagen)

PCR Molecular Size Marker contains a mixture of eight defined DNA fragments ranging from 50 to 2000 bp at convenient size intervals for characterising small DNA products. The markers are supplied in gel loading buffer containing two tracking dyes that do not interfere with UV illumination of ethidium bromide-stained bands. A separate vial of 6X Loading Buffer is included. Each vial of markers is sufficient for 50 lanes on TBE or TAE agarose stained with ethidium bromide. The recommended amount for loading (5 µl) produces bands of even intensity that are bright, sharp and easy to photograph.

Supplier: Cytiva

Enhanced chemiluminescence (ECL) detection reagents for reliable detection of medium and high expression proteins.

Supplier: Cytiva

Cy™ fluorescent amidites for incorporation of Cy3, Cy3,5, Cy5, Cy5,5 fluorescent dyes at any position in an oligonucleotide during synthesis.