Supplier: G-Biosciences

Western ReProbe™ is a single component system that is specifically formulated to dissociate and remove antibodies from membrane-bound proteins without destroying the antigenic binding affinity. Western ReProbe™ also enables the ability to reuse Western blots.

Supplier: Merck

Chemiluminescent HRP detection reagent for use on immunoblots, Western blotting, dot/slot blotting.

Supplier: G-Biosciences

These multiple tissue, single species blots permit researchers to visualise the tissue distribution of their protein in a particular species. These have been successfully used for the analysis of a wide variety of proteins. Each blot has multiple tissue types from either human, mouse or rat species.

Supplier: Rockland Immunochemicals

NPP ELISA alcaline phosphatase substrate is a chromogenic substrate used in immunosorbent assays.

Supplier: G-Biosciences

SWIFT™ transfer pads are treated with a proprietary electrolyte buffer to enhance Western blot transfer efficiency.

Supplier: G-Biosciences

Ready-to-screen, human, single tissue blots, from both normal and tumour tissues. G-Biosciences pre-made blots are prepared with the appropriate GenLysate™ and 50 μg protein is loaded onto a 4 to 20% denaturing polyacrylamide gel, along with a prestained molecular weight marker. After the proteins are resolved, they are transferred to a PVDF membrane, using our Western transfer buffer.

Supplier: G-Biosciences

G-Biosciences' Botanical Garden Blot is prepared using total protein extracted from a variety of 5 to 10 day old, whole plant tissues.

Supplier: Biotium

NucView® caspase-3 substrates are fluorescent probes that allow for the real-time detection of apoptosis by monitoring caspase-3/7 activity in intact cells using confocal microscopy, flow cytometry or microplate readers.

Supplier: G-Biosciences

This blot contains two protein lanes, a protein marker lane and a lane with one GenLysate™ sample. After the proteins are resolved, they are transferred to a PVDF membrane using Western Transfer Buffer. The blots are ready to be blocked and probed with the antibodies of choice.

Supplier: G-Biosciences

This blot contains two protein lanes, a protein marker lane and a lane with one GenLysate™ sample. After the proteins are resolved, they are transferred to a PVDF membrane using Western Transfer Buffer. The blots are ready to be blocked and probed with the antibodies of choice.

Supplier: G-Biosciences

These are ready to screen, normal tissue, heart tissue region blots from human and pig. The heart tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult pig and adult human hearts.

Supplier: G-Biosciences

G-Biosciences offers ready-to-screen, normal tissue, brain tissue region blots. The brain tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult mouse, rat, human, and primate brains.

Supplier: Biotium

Activated charcoal retains ethidium bromide, GelRed™ and GelGreen™ efficiently in a convenient bag for decontamination of used gel staining solutions. Each bag has a total capacity of at least 10 mg ethidium bromide.

Supplier: Merck

The Visualizer Western Blot Detection kit is designed for the sensitive detection of proteins in chemiluminescent Western blots.

Supplier: Merck

Western blotting is a commonly used technique for studying protein function and localisation. Typically, protein samples are electro-phoresed on SDS-page and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of southern and northern blots, it has been difficult to reuse western blots.

Supplier: Merck

Western blotting is a commonly used technique for studying protein function and localisation. Typically, protein samples are electro-phoresed on SDS page and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of southern and northern blots, it has been difficult to reuse western blots.

Supplier: Merck

Immobilon® Western HRP substrates are compatible with both PVDF and nitrocellulose membranes, as well as all commonly used buffers and blocking reagents.

Supplier: G-Biosciences

The Swift™ Western Diluent is a solution that reduces the blocking and antibody incubations on Western blot membranes to <60 minutes. Swift™ Western Diluent generates comparable results to traditional Western blotting procedures and other commercial “fast” Western blotting kits.

Supplier: G-Biosciences

Single tissue and multiple species blot contains four protein lanes with an identical GenLysate™ tissue lysate from human, mouse and rat species, and a protein marker lane.

Supplier: Merck

Western blotting is a commonly used technique for studying protein function and localisation. Typically, protein samples are electro-phoresed on SDS-page and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of southern and northern blots, it has been difficult to reuse western blots.

Supplier: VWR Chemicals

Protein Marker V (pre-stained) for monitoring protein separation, transfer efficiency and size determination.

Supplier: VWR Chemicals

EZ-Vision® 1 kb DNA ladder is a pre-mixture of DNA marker, loading buffer and a proprietary fluorescent DNA visualisation dye that can be applied directly to the gel. This non toxic, non mutagenic dye eliminates the need for ethidium bromide staining. The 1 kb ladder consists of 10 double-stranded bands with molecular weights between 200 and 10 000 bp. The intensity of individual bands varies to facilitate easy identification.

Supplier: VWR Chemicals

peqGOLD DNA ladder 100 bp plus is ideal for size determination in the range of 100 to 3000 base pairs. There are no unspecific bands besides the fragments described. 1000 and 500 bp bands have a higher DNA content and serve as a size reference.

Supplier: VWR Chemicals

peqGOLD pre-stained protein marker IV is a mixture of 10 recombinant, highly purified coloured proteins supplied 'ready to use' in loading buffer. The protein concentrations are optimised to yield well-defined bands directly visible in SDS-polyacrylamide gels.

Supplier: VWR Chemicals

PCR DNA Marker™ offers 8 fragments ranging from 50 to 2000 bp. The marker contains high intensity reference bands that are ideal for quick sizing of PCR products and restriction digests. Simply mix 10 μl of PCR DNA Marker™ with required quantity of loading buffer and load into well. There is sufficient material for 100 assays.

Supplier: VWR Chemicals

RiboReady™ Color 1 kb RNA ladder allows precise sizing of RNA molecules in denaturing acrylamide gels.

Supplier: VWR Chemicals

VWR® DNA ladders are supplied in a loading buffer that is ready to use on agarose and polyacrylamide DNA gels. The ladders are suitable with TBE, TAE, SB and LB electrophoresis systems.

Supplier: VWR Chemicals

Protein Marker II for accurate molecular weight determination of proteins by SDS-PAGE.

Supplier: VWR Chemicals

RiboReady™ Color Micro RNA ladder allows precise sizing of RNA molecules in denaturing acrylamide gels. The apparent sizes of bands in the RiboReady™ Color Micro RNA ladder are 100, 75, 50, 40, 30 and 20 bases in length.

Supplier: VWR Chemicals

DNA ladder for quantitative and qualitative analysis of dsDNA.