You searched for: Protein Expression Reagents
Since a successful protein expression is dependent on an efficient proteomic workflow, the reagents are designed to address all needs including induction of protein expression, cell lysis/extraction, capture, and purification. These substances are suitable for both small-scale and large-scale studies. Compatible with automated systems, the protein expression reagents enable regulated processing without monitoring the culture or adding any inducers during cell growth. A range of more specific reagents are available to fit any procedure.
TriLink® CleanCap® Cas9 Nickase mRNA (5 moU)
Supplier: TriLink BioTechnologies
Cas9 Nickase mRNA expresses a version of the Streptococcus pyogenes SF370 Cas9 protein (CRISPR Associated Protein 9) that contains a D10A amino acid substitution. This mRNA also contains a C-terminal nuclear localisation signal followed by a HA tag.
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TriLink® CleanCap® M6 FLuc mRNA (N1MePsU)
Supplier: TriLink BioTechnologies
The FLuc mRNA will express a luciferase protein, originally isolated from the firefly, Photinus pyralis. FLuc is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.
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TriLink® CleanCap® Cas9 mRNA
Supplier: TriLink BioTechnologies
Cas9 mRNA expresses a version of the Streptococcus pyogenes SF370 Cas9 protein (CRISPR Associated Protein 9). Cas9 functions as part of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) genome editing system. In the CRISPR system, an RNA guide sequence targets the site of interest and the Cas9 protein is employed to perform double stranded DNA cleavage.
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TriLink® CleanCap® FLuc mRNA (5 moU)
Supplier: TriLink BioTechnologies
The FLuc mRNA expresses a luciferase protein, originally isolated from the firefly, Photinus pyralis. FLuc is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.
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TriLink® CleanCap® Beta gal mRNA (5moU)
Supplier: TriLink BioTechnologies
β-galactosidase (β-gal), a protein product of the bacterial LacZ gene, catalyzes the conversion of β-galactosides into monosaccharides. β-gal is a common marker gene used to assess transfection efficiency.
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TriLink® CleanCap® OVA mRNA (5 moU)
Supplier: TriLink BioTechnologies
Ovalbumin (OVA) is a member of the serpin superfamily and the predominant glycoprotein found in egg whites. It is a commonly used antigen for immunisation and biochemical studies and is an established model allergen for airway hyper-responsiveness.
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ALiCE for Research
Supplier: LENIOBIO
From ALiCE® for Research to ALiCE® for Scale-Up Protein Expression, we offer cell-free protein expression products with radical results for every production setup.
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TriLink® CleanCap® M6 Cas9 mRNA (N1MePsU)
Supplier: TriLink BioTechnologies
Cas9 mRNA expresses a version of the Streptococcus pyogenes SF370 Cas9 protein (CRISPR Associated Protein 9). Cas9 functions as part of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) genome editing system. In the CRISPR system, an RNA guide sequence targets the site of interest and the Cas9 protein is employed to perform double-stranded DNA cleavage.
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ALiCE for Scale-Up
Supplier: LENIOBIO
ALiCE® - Linearly scalable eukaryotic cell-free protein expression. Scalable protein expression with ALiCE® works as effectively for assessment of future development as it does in the exploration phase. The same simple protocol applies across all scales. Add the DNA sequence and let ALiCE® make your protein.
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TriLink® CleanCap® EGFP mRNA
Supplier: TriLink BioTechnologies
The EGFP mRNA expresses an enhanced version of the green fluorescent protein, originally isolated from the jellyfish, Aequorea victoria. EGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm.
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TriLink® CleanCap® FLuc mRNA
Supplier: TriLink BioTechnologies
The FLuc mRNA expresses a luciferase protein, originally isolated from the firefly, Photinus pyralis. FLuc is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.
Expand 3 Items
TriLink® CleanCap® mCherry mRNA (5 moU)
Supplier: TriLink BioTechnologies
mCherry mRNA encodes the fluorescent protein, mCherry, which is derived from DsRed, a protein found in Discosoma sp. mCherry is a monomeric fluorophore with a peak absorption at 587 nm and emission at 610 nm. It is stable and resistant to photobleaching.
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Freund's adjuvants
Supplier: Rockland Immunochemicals
Freund's complete adjuvant
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TriLink® CleanCap® M6 EGFP mRNA (N1MePsU)
Supplier: TriLink BioTechnologies
The EGFP mRNA expresses an enhanced version of the green fluorescent protein, originally isolated from the jellyfish, Aequorea victoria. EGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm.
Expand 3 Items
TriLink® CleanCap® EGFP mRNA (5 moU)
Supplier: TriLink BioTechnologies
The EGFP mRNA expresses an enhanced version of the green fluorescent protein, originally isolated from the jellyfish, Aequorea victoria. EGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm.
Expand 3 Items
TriLink® CleanCap® Cas9 mRNA (5 moU)
Supplier: TriLink BioTechnologies
Cas9 mRNA expresses a version of the Streptococcus pyogenes SF370 Cas9 protein (CRISPR Associated Protein 9). Cas9 functions as part of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) genome editing system. In the CRISPR system, an RNA guide sequence targets the site of interest, and the Cas9 protein is employed to perform double-stranded DNA cleavage.
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TriLink® CleanCap® Cre mRNA (5 moU)
Supplier: TriLink BioTechnologies
NLS-Cre Recombinase mRNA is a capped and polyadenylated messenger RNA encoding Cre recombinase fused to a nuclear localisation sequence (NLS). Cre recombinase is a tyrosine recombinase that catalyzes recombination between two loxP sites.
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TriLink® CleanCap® OVA mRNA
Supplier: TriLink BioTechnologies
Ovalbumin (OVA) is a member of the serpin superfamily and the predominant glycoprotein found in egg whites. It is a commonly used antigen for immunisation and biochemical studies and is an established model allergen for airway hyper-responsiveness.
Expand 3 Items
TriLink® CleanCap® M6 Cre mRNA (N1MePsU)
Supplier: TriLink BioTechnologies
NLS-Cre Recombinase mRNA is a capped and polyadenylated messenger RNA encoding Cre recombinase fused to a nuclear localisation sequence (NLS). Cre recombinase is a tyrosine recombinase that catalyses recombination between two loxP sites.
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TriLink® CleanCap® M6 mCherry mRNA (N1MePsU)
Supplier: TriLink BioTechnologies
mCherry mRNA encodes the fluorescent protein, mCherry, which is derived from DsRed, a protein found in Discosoma sp. mCherry is a monomeric fluorophore with a peak absorption at 587 nm and emission at 610 nm. It is stable and resistant to photobleaching.
Expand 3 Items
TriLink® CleanCap® EPO mRNA (5moU)
Supplier: TriLink BioTechnologies
EPO mRNA encodes the human erythropoietin (EPO) protein, a hormone that controls erythropoiesis, or red blood cell production. EPO also plays a role in wound healing and the brain’s response to neural injury. Kariko et al. showed that transfection of EPO mRNA in vivo resulted in significant increases of both reticulocyte counts and hematocrits.
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TriLink® CleanCap® Beta gal mRNA
Supplier: TriLink BioTechnologies
β-galactosidase (β-gal), a protein product of the bacterial LacZ gene, catalyzes the conversion of β-galactosides into monosaccharides. β-gal is a common marker gene used to assess transfection efficiency.
Expand 3 Items
TriLink® CleanCap® Renilla Luc mRNA (5 moU)
Supplier: TriLink BioTechnologies
Renilla luciferase (Renilla Luc) is a bioluminescent reporter protein derived from Renilla reniformis (sea pansy). In the presence of the cofactor, coelenterazine, Renilla luciferase produces light. Renilla Luc mRNA can be used as an internal control in combination with FLuc mRNA to test different conditions, such as dosage or chemical modifications, and their effect on translation.
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TriLink® CleanCap® M6 EPO mRNA (N1MePsU)
Supplier: TriLink BioTechnologies
EPO mRNA encodes the human erythropoietin (EPO) protein, a hormone that controls erythropoiesis, or red blood cell production. EPO also plays a role in wound healing and the brain’s response to neural injury. Kariko et al. showed that transfection of EPO mRNA in vivo resulted in significant increases of both reticulocyte counts and hematocrits.
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Alum adjuvant, AlumVax phosphate
Supplier: OZ BIOSCIENCES
AlumVax phosphate is a wet gel (colloidal) of aluminium phosphate 2%. Aluminium salts are the most common adjuvants used in approved prophylactic vaccines because of their excellent safety profile and ability to enhance the protective humoral immune response.
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ProLite™ His-Tag Protein Gel Staining Kit
Supplier: AAT BIOQUEST
This kit requires very little hands-on time, allowing for rapid protein expression screening in a variety of gel types using an imaging equipment with a standard FITC filter.
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Freund's incomplete adjuvant, Imject™
Supplier: Thermo Fisher Scientific
Imject™ Freund's Incomplete Adjuvant (FIA) is a popular non specific stimulator of the immune response for use in antibody production immunisation protocols.
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Alum adjuvant, AlumVax hydroxide
Supplier: OZ BIOSCIENCES
AlumVax hydroxide is a wet gel (colloidal) of aluminium hydroxide 2%. It is the most common adjuvant used in approved prophylactic vaccines because of its excellent safety profile and ability to enhance the protective humoral immune response.
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Freund's complete adjuvant, CFAVax
Supplier: OZ BIOSCIENCES
Complete Freund’s Adjuvant (CFA) is a water-in-oil emulsion containing 1 mg per ml heat-killed Mycobacterium tuberculosis.
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Freund's complete adjuvant, Imject™
Supplier: Thermo Fisher Scientific
Imject™ Freund's Complete Adjuvant (FCA) is a popular non specific stimulator of the immune response for use in antibody production immunisation protocols.