You searched for: Nucleic Acid Reagents

Supplier: Thermo Fisher Scientific

ABsolute™ qPCR Capillary Mixes contain an optimised reaction buffer specifically designed to provide the highest possible sensitivity, specificity and consistent end-point values in real time instruments that use glass capillaries, such as the Roche LightCycler®. The 2X mixes contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes.

Supplier: Cytiva

For PCR and other procedures requiring DNA polymerase activity at elevated temperatures.

Supplier: Thermo Fisher Scientific

ABsolute Blue™ Master Mix incorporates an inert blue dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy and foolproof. Applications include gene expression, siRNA validation, SNP typing and genotyping.

Supplier: Thermo Fisher Scientific

The Verso™ RT-PCR system combines a reverse transcription enzyme, improved priming options and optimised buffers to generate high yield and full length cDNA. With a variety of kit options for both 1- and 2-step RT-PCR reactions, Verso™ provides optimum flexibility and sensitivity in an easy to use format.

Supplier: BIOPREMIER

This Supreme RT-PCR detection kit HLVd/LCV/CanCV is designed for the detection of Hop Latent Viroid (HLVd), Lettuce Chlorotic Virus (LCV) and Cannabis Cryptic Virus (CanCV) in a single PCR reaction (TETRAPLEX).

Supplier: Thermo Fisher Scientific

The Verso™ qRT-PCR system combines the Verso™ RT enzyme with ABsolute™ Blue qPCR master mix to provide maximum convenience and versatility. Available in a 1- or 2-step format, as well as multiple pack sizes, to suit all application and throughput requirements.

Supplier: Cytiva

illustra™ Hot Start Master Mix is a 2X premixed formulation that can effectively reduce non specific priming and primer-dimer formation during PCR. illustra™ Hot Start Master Mix uses a novel hot start method called primer sequestration.

Supplier: Cytiva

illustra™ Hot Start Mix, Ready-To-Go™ is a pre-formulated and pre-dispensed, freeze-dried PCR reagent mix, including high quality PuReTaq™ DNA polymerase and a hot start activator protein, for increased specificity and reproducibility of PCR amplifications. The Ready-To-Go™ beads are provided in either 0,5 or 0,2 ml tubes that are compatible with most thermal cyclers.

Supplier: Thermo Fisher Scientific

ABsolute™ qPCR low ROX mixes are optimised for use with real time instruments that are able to detect long wavelength dyes with greater sensitivity, such as the ABI 7500. The lower level of ROX still facilitates normalisation across the plate, while maintaining the sensitivity of the assay. The mixes contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes. The 2X qPCR mix contains a proprietary reaction buffer that provides high sensitivity, specificity and reproducibility.

Supplier: PRIMERDESIGN

This genesig® Easy qPCR detection kit targets the invasion protein (invA) gene from Salmonella spp. The kit is designed for the in vitro quantification of Salmonella spp genomes and to have a broad detection profile.

Supplier: MACHEREY-NAGEL

NucleoSpin® Plant II Midi/Maxi kits are designed for the isolation of genomic DNA from plant material and fungi. Plant II Midi allows processing of up to 400 mg starting material and Plant II Maxi up to 1500 mg.

Supplier: MACHEREY-NAGEL

NucleoSpin® RNA Midi spin kit is used for the isolation of RNA of highest integrity.

Supplier: MACHEREY-NAGEL

NucleoSpin® Gel and PCR Clean-up kits allow 2-in-1 clean up with optimised recovery and elution volume.

Supplier: MACHEREY-NAGEL

NucleoSpin® Plasmid is designed for the rapid, small scale preparation of high-purity plasmid DNA.

Supplier: MACHEREY-NAGEL

NucleoSpin® 8/96 Tissue and NucleoSpin® 8/96 Tissue Core kits for isolation of genomic DNA from human or animal tissue, cultured cells, or bacteria. Available with a choice of flexible 8-well strip format for varying throughput or 96-well format for high throughput.

Supplier: Beckman Coulter

This prep system kit isolates and purifies genomic DNA (gDNA) from whole blood. The kit is uses Solid Phase Reversible Immobilisation (SPRI) paramagnetic bead-based technology to effectively produce a high recovery of high quality DNA for downstream applications such as PCR.

Supplier: Merck

Activator solution, containing powerful activators like 4,5-dicyanoimidazole(DCI), 5-(3,5-bis(trifluoromethyl)phenyl)-1H-tetrazole (Activator 42) or5-(Ethylthio)-1H-tetrazole (ETT) in acetonitrile, are mixed with solutions ofphosphoramidites during the extension step. The activator reacts with theamidite group to form a highly reactive intermediate. The intermediate thenforms an internucleotide bond with the detritylated 5′hydroxyl group of thegrowing oligonucleotide chain.

Supplier: MACHEREY-NAGEL

NucleoSpin® Tissue genomic DNA kit, 10 preps

Supplier: MACHEREY-NAGEL

NucleoSpin® RNA mini spin kit for the isolation of RNA of the highest integrity.

Supplier: STEMCELL Technologies

For purification of RNA from cells.

Supplier: BIOO SCIENTIFIC

The CRISPRclean® stranded total RNA prep with rRNA depletion (HMR) is a total RNA library prep leveraging a CRISPR-powered workflow facilitating the detection of lower expressing and biologically relevant transcripts from complex samples.

Supplier: NEW ENGLAND BIOLABS

The NEBExpress Cell-free E. coli Protein Synthesis System is an extract-based transcription/translation system derived from E. coli cells that have been engineered for high in vitro synthesis performance. It is designed to synthesize proteins ranging in size from 17 to 230 kDa in just 2 to 4 hours under the control of T7 RNA Polymerase.

Supplier: STEMCELL Technologies

Immunomagnetic extraction of total nucleic acid (DNA and RNA).

Supplier: STEMCELL Technologies

For purification of genomic DNA from cells or tissue.

Supplier: Beckman Coulter

The Agencourt AMPure XP system is a highly efficient, easily automated PCR purification system that delivers superior quality DNA with no salt carryover.

Supplier: BIOO SCIENTIFIC

The CRISPRclean® stranded total RNA prep with rRNA depletion (HMR + pan bacteria) is a total RNA library prep leveraging a CRISPR-powered workflow facilitating the detection of lower expressing and biologically relevant transcripts from complex samples.

Supplier: ROCHE

The High Pure Viral Nucleic Acid Kit is intended for general laboratory use and efficiently purifies viral nucleic acids from serum, plasma, or whole blood using sample volumes of up to 200 μl. When using whole blood as starting material, total nucleic acids are isolated, including viral nucleic acids. The purified viral nucleic acid is eluted in nuclease-free water, and is suitable for direct use in PCR and RT-PCR.

Supplier: STEMCELL Technologies

For extraction of DNA from agarose gels, or clean-up of PCR products and restriction enzyme digestions.

Supplier: EPICENTRE

Purchase individual FailSafe™ DNA polymerase and/or FailSafe™ 2X PreMix Buffers here.

Supplier: NEW ENGLAND BIOLABS

PaqCI is a Type IIS restriction enzyme with a 7 basepair recognition sequence: CACCTGC(⁴/₈).