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Supplier: ENZO LIFE SCIENCES

Optimised and efficient cDNA synthesis and dye-based qPCR in a single tube.

Supplier: Quantabio

AccuStart™ Genotyping ToughMix® ist ein 2X konzentrierter, gebrauchsfertiger Reaktionscocktail für die Amplifikation von DNA-Templates, der Inhibitoren widersteht, die sich häufig in Rohproben aus dem Umweltbereich, in Pflanzen- oder Tiergeweben finden. Es handelt sich um ein robustes PCR-Reagenz, das eine hoch spezifische und effiziente Amplifikation für Genotypisierungs-Sequenzvariation ermöglicht, einschließlich allelischer Diskriminierung von Einzelnukleotid-Polymorphismen (SNP) unter Verwendung zahlreicher Fluorogensondensysteme wie TaqMan® Hydrolysefühlern.

Supplier: Quantabio

PerfeCTa® MultiPlex qPCR ToughMix® ist ein 5-fach konzentrierter gebrauchsfertiger Reaktionscocktail für quantitative Echtzeit-PCR (qPCR). Er widersteht vielen bekannten Inhibitoren von PCR, die sich häufig in Rohproben aus dem Umweltbereich, in Pflanzen- oder in Tiergeweben finden. Er enthält alle Komponenten außer Primer, Sonden und Schablonen. Der 5-fach konzentrierte ToughMix® ermöglicht das Hinzufügen größerer Mengen von Templates und eine verbesserte Erkennungsempfindlichkeit bei Proben mit geringer Konzentration. PerfeCTa® MultiPlex qPCR ToughMix® wurde für maximale PCR-Effizienz, Empfindlichkeit und Spezifität in reduzierten Reaktionsvolumina mit schnellen Zykluszeiten oder bei konventionellen PCR-Cycling-Protokollen optimiert.

Supplier: Quantabio

Ultra-Plex™ 1-Step ToughMix® is a ready-to-use, 4X-concentrated master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridisation probe detection chemistries such as TaqMan® 5’-hydrolysis probes on Real-Time PCR systems that utilise ROX passive reference with 490 nM excitation. First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. It is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets in uni- or multiplexed RT-qPCR applications as well as high throughput gene-expression studies. The system has been optimised to deliver maximum RT-qPCR efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times. Ultra-Plex™ 1-Step ToughMix® contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries.

Supplier: Quantabio

qScript™ One-Step SYBR® Green qRT-PCR Kits reliably deliver maximum qRT-PCR efficiency, sensitivity and specificity in with a simplified protocol. These kits offer reliable and unbiased amplification of all RNA targets regardless of abundance. The proprietary supermix maximises activities of both reverse transcriptase and AccuStart™ Taq DNA polymerase without compromising specificity of amplification. qScript™ One-Step SYBR® Green qRT-PCR kits are available and optimised for all real-time PCR instrument platforms including those requiring normalisation with ROX reference dye.

Supplier: Quantabio

PerfeCTa® PreAmp SuperMix ist ein 5-fach konzentrierter, gebrauchsfertiger Reaktionscocktail für die eindeutige, ausgewählte Anreicherung von Zielmolekülsequenzen aus begrenzenden Mengen von Ausgangsmaterial für Downstream-Genexpressions-Profiling oder gezielte erneute Sequenzierungen. Er enthält alle Komponenten, mit Ausnahme von Primern und Schablonen. Der 5-fach konzentrierte SuperMix ermöglicht auch höhere Schablonenvolumen bei der Arbeit mit Proben geringer Konzentration und/oder reduzierten Reaktionsvolumen. Der inerte hellblaue Farbmarker erleichtert die Darstellung kleiner Reaktionsvolumen und ermöglicht genaues Pipettieren.

Supplier: AGILENT

High performance, ultra-sensitive, SYBR® Green QPCR master mix reagent with ROX concentration for reliable quantification across a wide range of targets and templates.

Supplier: ENZO LIFE SCIENCES

Optimised standard PCR mix for enhanced speed, yield, and specificity. Used for standard PCR applications.

Supplier: ENZO LIFE SCIENCES

Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.

Supplier: Biotium

EvaGreen® Dye and high-performance dye-based qPCR master mixes containing EvaGreen® qPCR dye, Cheetah™ HotStart Taq Polymerase, and Forget-Me-Not™ tracking dye. Available with 2-colour tracking or pre-mixed with low or high ROX.

Supplier: Quantabio

Superior sensitivity and multiplexing for DNA amplification of long targets.

Supplier: Genscript

Taq DNA polymerase is a thermostable DNA polymerase isolated from an E. coli strain that carries the Taq DNA polymerase gene.

Supplier: Biotium

A qPCR master mix containing Evagreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, suitable for qPCR using a fast cycling protocol.

Supplier: Quantabio

PerfeCTa® SYBR® Green SuperMixes und FastMixes™ ermöglichen effiziente, empfindliche und präzise quantitative PCR mit proprietären Puffern und SYBR® Green Stabilisatoren, die das Fluoreszenzsignal und die PCR-Effizienz maximieren und Primerdimeren verringern. Die SuperMixes und FastMixes™ wurden für alle Echtzeit-PCR-Instrumentenplattformen optimiert, einschließlich der Plattformen, bei denen eine Normalisierung mit ROX-Referenzfarbstoff oder Fluorescin erforderlich ist.

Supplier: Quantabio

The AccuStart™ II Mouse Genotyping kit is designed for fast and easy preparation of PCR-ready DNA extracts and endpoint PCR analysis from tissues such as tail snips and ear punches commonly used for genotyping knockout and transgenic animals. The kit combines Extracta™ DNA Prep for PCR - Tissue with AccuStart™ II GelTrack™ PCR SuperMix in a single kit.

Supplier: Quantabio

PerfeCTa® qPCR FastMix® II is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments. It is a versatile and robust solution that provides the ultimate sensitivity and high PCR efficiency using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. PerfeCTa® qPCR FastMix® II is provided as a 2X concentrated ready to use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. The light blue colour of the AccuVue™ tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimise pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.

Supplier: AGILENT

Paq5000 is capable of quickly and robustly amplifying up to 6 kB genomic DNA targets.

Supplier: AGILENT

Detects low copy number target and withstands PCR inhibitors without extensive purification.

Supplier: AGILENT

Improves reliability and yield of conventional Taq-based PCR amplifications.

Supplier: AGILENT

High performance, ultra-sensitive, probe QPCR master mix reagent with high or low ROX concentration for reliable quantification across a wide range of targets and templates.

Supplier: ENZO LIFE SCIENCES

Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.

Supplier: Biotium

Kits designed to make it simple to start performing viability PCR with the choice of viability dye (PMA or PMAxx™).

Supplier: Quantabio

Diese Kits bieten eine praktische und hochempfindliche Lösung für reverse Transkriptase-PCR (qRT-PCR) der RNA-Templates in Echtzeit unter Verwendung der Hybridisierungssonden für chemische Nachweisverfahren wie TaqMan® 5'-Hydrolyse-Proben.

Supplier: Cytiva

High purity dNTPs for amplification, dideoxy sequencing, labelling, mutagenesis, cDNA synthesis, and expression profiling.

Supplier: Cytiva

illustra™ dNTPs are high purity solutions for enzymatically sensitive applications, such as nucleic acid amplification, sequencing, labelling, mutagenesis, cDNA synthesis and expression profiling.

Supplier: Quantabio

AccuStart™ II Taq DNA Polymerase is a high purity, recombinant Taq DNA polymerase preparation with high avidity monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly. Non-specific extension of primers at low temperatures is a common cause of artifacts and poor sensitivity in PCR. The AccuStart™ II automatic hot-start enables specific and efficient primer extension in the PCR process with the added convenience of room temperature reaction assembly. The included 10X PCR Buffer II is a new optimised buffer that provides higher product yield, improved specificity, and enhanced multiplexing capability.

Supplier: Quantabio

PerfeCTa® qPCR SuperMixes sind 2X konzentrierte, gebrauchsfertige Reaktionscocktails, die alle Komponenten außer Primern, Sonde und Vorlage für qPCR enthalten.

Supplier: Biotium

Cheetah™ Taq is a chemically modified hot-start Taq DNA polymerase useful for preventing or reducing nonspecific DNA amplification in PCR. Modified using a novel modifying reagent, Cheetah™ Taq represents a major improvement upon AmpliTaq Gold by having a faster activation time and better shelf-life.

Supplier: AGILENT

PfuUltra High-Fidelity DNA Polymerase AD features a reformulated buffer system for increased economy with the same high performance as the original PfuUltra DNA Polymerase for robust, ultra-high-fidelity PCR.

Supplier: Corning

AxyPrep™ Mag Plasmid Purification Kit utilises a magnetic bead technology for high throughput purification of plasmid DNA from E.coli cells. The AxyPrep™ Mag kit can also be used with fosmid and BAC vector-based constructs ranging from 5 to 150 Kb. The system consists of steps of pelleting cells, resuspension, lysis, neutralisation, DNA binding, ethanol washing and plasmid elution. The protocol is automation-compatible.