You searched for: Nucleic Acid Reagents
Nucleic acid reagents form the foundation of genetic analysis and molecular biology, playing a pivotal role from cloning to gene expression studies. Delve into our collection featuring high-fidelity enzymes for end-point PCR, advanced reagents for isothermal amplification, and comprehensive kits for nucleic acid purification. For precision in quantitative assays, explore our selection of qPCR and RT-qPCR kits, primed for unparalleled accuracy. Our portfolio also includes next-generation sequencing reagents, offering cutting-edge solutions for genomic sequencing and personalized medicine advancements.
PerfeCTa® qPCR ToughMix®
Supplier: Quantabio
PerfeCTa® qPCR ToughMix® is a 2X concentrated ready to use reaction cocktail for PCR amplification of DNA templates that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues or animal tissues. It is a versatile and robust Real-Time qPCR reagent that provides maximum sensitivity and PCR efficiency with a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. PerfeCTa® qPCR ToughMix® contains all required reaction components, except primers, probe(s) and DNA template. The light blue colour of the AccuVue™ tracer dye simplifies reaction assembly in white, or clear plates and helps to minimise pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.
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qPCR SuperMix, PerfeCTa® MultiPlex
Supplier: Quantabio
This SuperMix is a 2X concentrated, ready to use reaction cocktail for Real-Time qPCR that contains all components, except primers, probes, and templates. The system transcends multiplex limitations of conventional PCR master mixes, enabling unbiased amplification of up to five target sequences in a single tube. Suppression of low copy amplicons by high copy reference targets in the amplification is a common problem in multiplex PCR. This can skew, or mask the apparent representation and quantification of low copy target sequences. PerfeCTa® MultiPlex qPCR SuperMix delivers dynamic range and sensitivity to multiplexed qPCR that is comparable to that for singleplex qPCR probe assays without the need for limiting or variable primer concentrations.
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AccuStart™ II PCR Genotyping Kit
Supplier: Quantabio
The AccuStart™ II Mouse Genotyping kit is designed for fast and easy preparation of PCR-ready DNA extracts and endpoint PCR analysis from tissues such as tail snips and ear punches commonly used for genotyping knockout and transgenic animals. The kit combines Extracta™ DNA Prep for PCR - Tissue with AccuStart™ II GelTrack™ PCR SuperMix in a single kit.
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PerfeCTa® QPCR mixes, SYBR® green super mixes and fast mixes
Supplier: Quantabio
PerfeCTa® SYBR® Green SuperMixes and FastMixes™ enable efficient, sensitive and precise quantitative PCR with proprietary buffers and SYBR® Green stabilisers that maximise fluorescent signal, PCR efficiency, and reduce primer dimers. These SuperMixes and FastMixes™ have been optimised for all Real-Time PCR instrument platforms, including those requiring normalisation with ROX reference dye or fluorescein.
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dNTP sets (dA, dC, dG, dT) 100 mM solutions
Supplier: G-Biosciences
Set consists of 100 mM aqueous solutions of dATP, dCTP, dGTP and dTTP each supplied in a separate vial. Also available separately.
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PerfeCTa® qPCR FastMix® II
Supplier: Quantabio
PerfeCTa® qPCR FastMix® II is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments. It is a versatile and robust solution that provides the ultimate sensitivity and high PCR efficiency using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. PerfeCTa® qPCR FastMix® II is provided as a 2X concentrated ready to use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. The light blue colour of the AccuVue™ tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimise pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.
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AccuStart™ II GelTrack PCR SuperMix
Supplier: Quantabio
AccuStart™ II GelTrack PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb followed by analysis on agarose gels. It contains all components, except primers and template. AccuStart™ II GelTrack PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. The supermix includes electrophoresis tracking dyes that migrate at approximately 4 kb and 50 bp to allow direct loading of PCR product on agarose gels following amplification. AccuStart™ II Taq DNA polymerase in the master mix is inactivated with monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.
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Perfect Match PCR enhancers
Supplier: AGILENT
Increases specificity and yield of primary PCR amplification products.
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AMPIGENE® Taq DNA polymerase
Supplier: ENZO LIFE SCIENCES
Optimised standard PCR for enhanced speed, yield, and specificity.
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AMPIGENE® HS Taq DNA Polymerase
Supplier: ENZO LIFE SCIENCES
Increased sensitivity standard PCR for a broader range of samples, with enhanced speed, yield, and specificity.
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Qscript lyo 1-step
Supplier: Quantabio
Qscript lyo 1-step is a lyophilised single-reaction reagent optimised for highly sensitive and reproducible one-step RT-qPCR using hydrolysis probes.
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Fusion DNA polymerases, Herculase II
Supplier: AGILENT
Herculase II Fusion DNA Polymerases produce superior yields with extension times as short as 15 s/kb, even with genomic DNA targets up to 23 kb.
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Virus detection kit, qScript™ 1-Step Virus ToughMix®
Supplier: Quantabio
Superior sensitivity for viral RNA detection.
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dNTP mix
Supplier: G-Biosciences
Aqueous mixture of dATP, dCTP, dGTP and dTTP each at a final concentration of 10 or 25 mM.
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Digoxigenin-dUTP, alkali stable
Supplier: Biotium
Digoxigenin-dUTP, alkali stable
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Brilliant II probe MM with ROX
Supplier: AGILENT
Brilliant II QPCR and QRT-PCR reagents were developed to maintain the high quality of original Brilliant product line, but with a significant improvement in sensitivity of detection, ensuring reproducible quantification even at low target concentrations.
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PerfeCTa® MultiPlex qPCR SuperMix, low ROX
Supplier: Quantabio
PerfeCTa® Multiplex qPCR SuperMix, low ROX is a 2X concentrated, ready to use reaction cocktail that contains all components, except primers, probe(s), and template for real time qPCR on Applied Biosystems 7500 or Stratagene MX series of real time PCR systems.
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dNTP mix, 10 mM
Supplier: Merck Millipore (Novagen)
The 10 mM dNTP mix is a ready-to-use preparation of ultrapure dATP, dCTP, dGTP, and dTTP (monosodium salts) at a concentration of 10 mm each in sterile deionised water at pH 7,0. Qualified for any application that requires pure deoxynucleotides, such as PCR, cDNA synthesis, and fill-in reactions.
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dNTP mix
Supplier: Quantabio
The 10 mM dNTP mix is a solution of high purity deoxyribonucleoside triphosphates that has been functionally qualified for real-time quantitative PCR (qPCR). It is suitable for use in conventional end-point PCR, real time qPCR, first-strand cDNA synthesis, as well as other applications that require dNTP as substrate. The use of qPCR certified dNTPs is a simple but important measure to ensure consistent assay performance.
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Flatpack chambers
Supplier: BTX
Flatpack chambers are primarily used for prokaryotic applications (bacterial or yeast transformations), however they are also often used for high efficiency stem cell transfection.
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Yeast transformation kit, Fast Yeast Transformation™
Supplier: G-Biosciences
Fast Yeast Transformation™ is a rapid single step yeast transformation kit that takes less than 10 minutes to prepare competent yeast cells. The competent yeast cells can be used immediately or frozen for later use. This method is suitable for both circular and linear plasmid transformations.
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Electroporation solution kits, BTXpress™
Supplier: BTX
The BTXpress™ is a single buffer solution, developed to quickly and efficiently deliver genes into mammalian cells that were previously considered hard to transfect by chemical and other non viral methods. Transfection using this high performance electroporation solution is equally effective in delivering DNA as well as siRNA into mammalian cells.
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AccuStart™ II PCR SuperMix
Supplier: Quantabio
AccuStart™ II PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb. It contains all components, except primers and template. AccuStart™ II PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. A key component is AccuStart™ II Taq DNA polymerase which contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.
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qScript™ One-Step qRT-PCR kits
Supplier: Quantabio
Synthesis of cDNA and PCR amplification are carried out in the same tube without opening between procedures. These kits are optimised to deliver maximum RT-PCR efficiency and specificity, enabling unbiased co-amplification of low copy transcripts in the presence of higher copy reference genes. As a result the qScript™ One-Step qRT-PCR Kits offer improved sensitivity over other commercially available qPCR reagents. The qScript™ One-Step Master Mix maximises the activities of both reverse transcriptase and PCR enzymes, while minimising primer-dimer formation. High specificity is assured with AccuStart™ Taq DNA Polymerase, which is bound by monoclonal antibodies for superior hot-start. Upon heating to 95 °C, the antibodies denature irreversibly releasing fully active and unmodified Taq DNA polymerase. qScript™ One-Step qRT-PCR Kits are available for all Real-Time PCR instrument platforms, including those requiring normalisation with ROX reference dye.
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PCR primers, Mouse Ig-primer set
Supplier: Merck Millipore (Novagen)
The Mouse Ig-Primer Set is designed for amplification of immunoglobulin (Ig) variable region cDNAs. Mammalian light- and heavy-chain Igs contain conserved regions adjacent to the hypervariable complementarity defining regions (CDRs). Appropriately designed oligonucleotide primer sets enable these regions to be specifically amplified using PCR, after which the products may be sequenced directly or cloned.
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One step RT-PCR master mix kit
Supplier: Merck Millipore (Novagen)
The One Step RT-PCR Master Mix Kit allows rapid, sensitive analysis of gene expression from tissues and cells. It can replace methods for detecting and quantifying gene expression such as Northern blots, in situ hybridisation, dot blots, S nuclease assays and conventional two step RT-PCR. The kit utilises recombinant Thermus thermophilus (rTth) DNA Polymerase, which acts as both a thermostable RNA dependent DNA polymerase and a DNA dependent DNA polymerase. The rTth DNA Polymerase is provided in a 2X master mix with an antibody for antibody-mediated hot start, optimised buffer, and ultrapure deoxynucleotides. Antibody-mediated Hot Start enhances specificity of both reverse transcription and PCR. The kit enables cDNA synthesis from input RNA followed by PCR amplification of the cDNA in a single reaction, with no additional hands-on requirement for buffer changes or adding reagents. Typically, detection of a specific transcript requires only 2 hours.
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PCR clean-up reagent, Sera-Mag™ select
Supplier: Cytiva
Sera-Mag Select PCR clean-up, size selection reagent combines the exceptional binding characteristics of Sera-Mag carboxyl speedbeads with an optimised binding solution in a ready to use formulation. The DNA size range isolated from the process can be tailored to suit the user’s end requirements by adjusting the amount of reagent that is mixed with a fixed volume of sample.
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Magbeads FastDNA/RNA kit for virus, 192 preparations, MP Biomedicals
Supplier: MP Biomedicals
MagBeads FastDNA/RNA Kit for Virus is intended for extracting total viral nucleic acid from cell-free/low-cell content biological samples such as body fluids, serum, plasma, soaking solution, tissue homogenate, and culture supernatant.
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DNA clean up, Sephadex® G-100 DNA grade
Supplier: Cytiva
Sephadex™ G-100 DNA Grade is ideal for use in preparing spin columns for DNA purification.
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DNA extraction, illustra™ Nucleon™ PhytoPure™
Supplier: Cytiva
Nucleon™ PhytoPure™ enables rapid chloroform extraction of high-quality, high molecular weight genomic DNA from plant and fungal samples with efficient removal of protein as well as polysaccharides.