You searched for: Nucleic Acid Reagents

Supplier: Thermo Fisher Scientific

Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Supplier: Quantabio

Qscript lyo 1-step is a lyophilised single-reaction reagent optimised for highly sensitive and reproducible one-step RT-qPCR using hydrolysis probes.

Supplier: AGILENT

High performance, ultra-sensitive, SYBR® Green QPCR master mix reagent with ROX concentration for reliable quantification across a wide range of targets and templates.

Supplier: TriLink BioTechnologies

2',3'-Dideoxyadenosine-5'-O-(1-Thiotriphosphate) is a sugar modified nucleoside triphosphate where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. This ddATP analog also contains a 1-Thiotriphosphate which results in a nuclease resistant phosphorothioate linkage. The inability of polymerases to extend from a dideoxy nucleotide causes chain termination and is useful in antiviral research and in a variety of biotechnology applications.

Supplier: TriLink BioTechnologies

TriLink's patented CleanCap® Reagent AG (3' OMe) is a co-transcriptional capping reagent for in vitro transcription of 5' capped mRNA resulting in a Cap 1 structure with >95% capping efficiency.

Supplier: Biotium

A qPCR master mix containing Evagreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, suitable for qPCR using a fast cycling protocol.

Supplier: TriLink BioTechnologies

CleanCap® Reagent AG is TriLink’s patented co-transcriptional capping reagent for in vitro transcription of 5’ capped mRNA resulting in a Cap 1 structure. CleanCap has shown to provide up to 98% capping efficiency.

Supplier: Thermo Fisher Scientific

T7 RNA polymerase is ideal for synthesis of unlabeled and labeled RNA. It catalyses the 5'→3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter. It is also used in the study of RNA secondary structure and RNA-protein interactions, RNA splicing.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Supplier: AGILENT

The MycoSensor PCR and qPCR assay kits detect cultured Mycoplasma infection by real-time qPCR or PCR.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Supplier: Thermo Fisher Scientific

AATTC sites and cuts best at 37 °C in its own unique buffer. Exhibit 100% activity in the recommended buffer and reaction conditions.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Supplier: Thermo Fisher Scientific

CCT sites and cuts best at 37 °C in B buffer (Isoschizomers: AatI, PceI, SseBI, StuI).

Supplier: AGILENT

Brilliant II SYBR® QPCR and QRT-PCR reagents were developed for improved sensitivity of detection, ensuring reproducible quantification even at low target concentrations.

Supplier: AGILENT

The brilliant multiplex QPCR master mix allows you to amplify up to four targets in a single real-time PCR Reaction.

Supplier: Quantabio

qScript™ cDNA synthesis kit contains qScript™ Reverse Transcriptase, a mixture of a MMLV reverse transcriptase derivative and ribonuclease inhibitor protein, optimised for sensitive and reliable cDNA synthesis over a broad dynamic range of input RNA. cDNA synthesis reagents are provided in a variety of SuperMix and kit formats to suit specific applications.

Supplier: VWR Chemicals

Fast HiFi DNA Polymerase 2 U/µl is a proofreading DNA polymerase displaying the following features; high fidelity >60X Taq DNA Polymerase, ability to amplify problematic DNA targets, such as those with low to high GC content and ability to perform amplification on long DNA targets. lt is recommended for applications, which require extremely high fidelity such as cloning / sub cloning, NGS applications and mutagenesis.

Supplier: AGILENT

This DNA polymerase is ideal for a variety of techniques requiring high-fidelity DNA synthesis by the polymerase chain reaction (PCR).

Supplier: AGILENT

High performance, ultra-sensitive, probe QPCR master mix reagent with high or low ROX concentration for reliable quantification across a wide range of targets and templates.

Supplier: TriLink BioTechnologies

Biotin-16-Aminoallyl-2'-deoxyuridine can be enzymatically incorporated into DNA via PCR, nick translation, primer extension using Klenow(exo-) DNA polymerase, and terminal deoxynucleotidyl transferase (TdT). Biotinylated cDNA can be prepared with this NTP using Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase.

Supplier: Biotium

EvaGreen® Dye and high-performance dye-based qPCR master mixes containing EvaGreen® qPCR dye, Cheetah™ HotStart Taq Polymerase, and Forget-Me-Not™ tracking dye. Available with 2-colour tracking or pre-mixed with low or high ROX.

Supplier: AGILENT

Herculase II Fusion DNA Polymerases produce superior yields with extension times as short as 15 s/kb, even with genomic DNA targets up to 23 kb.

Supplier: OMEGA BIO-TEK

RNA-Solv® Reagent is a one reagent system for the isolation of total RNA from cells and tissues. The reagent, a single-phase solution consisting of phenol and guanidine isothiocyanate, is a modification of the single-step RNA isolation method developed by Chomczynski and Sacchi. The sample is homogenised and lysed in RNA-Solv® Reagent, which maintains the integrity of the RNA while disrupting and denaturing endogenous RNases and other cellular components. Extraction of the lysate with chloroform further denatures proteins and separates the mixture into an organic and an aqueous phase. RNA remains exclusively in the aqueous phase, and is subsequently recovered by isopropanol.

Supplier: AGILENT

Detects low copy number target and withstands PCR inhibitors without extensive purification.

Supplier: ENZO LIFE SCIENCES

Cyanine 3-UTP (Enhanced) can replace UTP as a substrate for T7 RNA polymerase in labeling systems that generate labeled probes through in vitro transcription. Probes generated by these methods are suitable for multicolor fluorescence analysis, specifically dual-color expression arrays in conjunction with cyanine 3-UTP .

Supplier: TriLink BioTechnologies

TriLink's patented CleanCap® Reagent M6 [CleanCap m6AG (3′ OMe)], is designed for the co-transcriptional capping of mRNA to produce an mRNA with base-modified Cap 1. Cap-1 mRNAs have superior in vivo activity compared to Cap-0 mRNAs produced by legacy capping methods.

Supplier: AGILENT

Brilliant III QPCR and QRT-PCR master mixes increase overall sensitivity and speed through the novel, quick-activating hot start and the engineered Taq-mutant.