You searched for: Nucleic Acid Reagents

Supplier: Thermo Fisher Scientific

CCNGGA sites and cuts best at 37 °C in Tango buffer.

Supplier: AGILENT

High performance, ultra-sensitive, SYBR® Green QPCR master mix reagent with ROX concentration for reliable quantification across a wide range of targets and templates.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Supplier: TriLink BioTechnologies

This nucleotide consists of an unmodified base, a deoxyribose, and a 5′ triphosphate group for synthesis of DNA molecules.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Supplier: TriLink BioTechnologies

2',3'-Dideoxyadenosine-5'-O-(1-Thiotriphosphate) is a sugar modified nucleoside triphosphate where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. This ddATP analog also contains a 1-Thiotriphosphate which results in a nuclease resistant phosphorothioate linkage. The inability of polymerases to extend from a dideoxy nucleotide causes chain termination and is useful in antiviral research and in a variety of biotechnology applications.

Supplier: AGILENT

PfuUltra High-Fidelity DNA Polymerase AD features a reformulated buffer system for increased economy with the same high performance as the original PfuUltra DNA Polymerase for robust, ultra-high-fidelity PCR.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Supplier: ENZO LIFE SCIENCES

Optimised and efficient cDNA synthesis and dye-based qPCR in a single tube.

Supplier: Biotium

A qPCR master mix containing Evagreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, suitable for qPCR using a fast cycling protocol.

Supplier: ENZO LIFE SCIENCES

Terminal labeling is an ideal procedure for the labeling of oligonucleotides with haptens such as biotin or fluorescein. An oligonucleotide can be synthesized using standard, commercially available reagents and labeled after synthesis in a simple and reproducible enzyme reaction.

Supplier: Biotium

Kits designed to make it simple to start performing viability PCR with the choice of viability dye (PMA or PMAxx™).

Supplier: Thermo Fisher Scientific

T7 RNA polymerase is ideal for synthesis of unlabeled and labeled RNA. It catalyses the 5'→3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter. It is also used in the study of RNA secondary structure and RNA-protein interactions, RNA splicing.

Supplier: VWR Chemicals

VWR® TEMPase Hot Start DNA Polymerases are highly stable polymerases, featuring higher specificity, superior sensitivity and greater yields compared to standard DNA polymerases. These features make them well suited for the detection of low abundance targets. Other uses include screening, amplification of GC-rich sequences, multiplex PCR, direct PCR and qPCR.

Supplier: AGILENT

Brilliant II QPCR and QRT-PCR reagents were developed for improved sensitivity of detection, ensuring reproducible quantification even at low target concentrations.

Supplier: ENZO LIFE SCIENCES

Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.

Supplier: AGILENT

Paq5000 is capable of quickly and robustly amplifying up to 6 kB genomic DNA targets.

Supplier: VWR Chemicals

An optimal buffer system is critical for the performance of successful PCR. VWR® Taq DNA Polymerase kits generally include two different buffers, Key Buffer and Extra Buffer, which are suited for different PCR needs. All buffers contain Tris and 15 mM MgCl₂ (1,5 mM MgCl₂ final concentration). Additional MgCl₂ for easy optimisation is included in a separate vial.

Supplier: Biotium

Highly purified dNTPs (>99% by HPLC) in purified water. Each deoxynucleotide solution (dATP, dCTP, dGTP, dTTP) is provided in a separate vial at 100 mm.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Supplier: Biotium

A qPCR master mix containing Cheetah™ Taq hotstart DNA polymerase, suitable for probe-based real-time PCR applications.

Supplier: ENZO LIFE SCIENCES

Rapid removal of contaminating DNA in PCR mastermixes, without reduction in PCR sensitivity.

Supplier: TriLink BioTechnologies

Biotin-16-Aminoallyluridine-5'-Triphosphate (Biotin-16-UTP, Biotin-16-AA-UTP) can be incorporated via a number of RNA polymerases, including SP6-, T3- and T7-RNA polymerase to form labeled RNA.

Supplier: VWR Chemicals

This fast extract genotyping PCR kit consists of a fast extraction DNA solution and VWR® Red Taq DNA polymerase 2X master mix. It is ideal for genotyping of DNA extracted from mammalian tissues, plant material, saliva or bacteria, providing PCR-ready DNA in 8 minutes and genotyping results in less than 1,5 hours.

Supplier: AGILENT

The Brilliant HRM Ultra-Fast Loci Master Mix for High Resolution Melt (HRM) analysis.

Supplier: AGILENT

The universal reverse primer serves as the downstream primer in the qPCR reaction by annealing to a universal sequence tag that is added to the cDNA during the reverse transcription protocol.

Supplier: AGILENT

Polyadenylation of miRNA from total RNA and synthesis of first-strand cDNA from tailed miRNAs.

Supplier: AGILENT

These QuikChange II kits provide improved fidelity, while maintaining greater than 80% mutation efficiency for single site mutagenesis.