You searched for: Protein Purification
Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.
Co-immunoprecipitation (Co-IP) Kit, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Co-Immunoprecipitation (Co-IP) Kit provides covalent antibody immobilisation and all the components necessary to perform a properly controlled co-IP experiment without antibody interference in the final product.
Expand 1 Items
Enterokinase cleavage capture kit
Supplier: Merck Millipore (Novagen)
The Enterokinase Cleavage Capture Kit is designed for highly specific cleavage of fusion proteins followed by rapid, affinity-based capture and removal of enterokinase. Following cleavage of the target protein, rEK is removed with >99% efficiency from the reaction by affinity capture on EKapture™ Agarose. Following capture of rEK, the EKapture Agarose is removed by spin filtration, because the same buffer conditions are used for both cleavage and capture no buffer changes are necessary.
Expand 1 Items
Pegylation reagents, methyl-PEG-amine compounds
Supplier: Thermo Fisher Scientific
The methyl-PEGn-amine (MA[PEG]n) PEGylation reagents are methyl ether-terminated PEG amines that are used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers. These PEGylation reagents are homogenous compounds of defined molecular weight and spacer length, providing precision in optimising modification applications.
Expand 5 Items
Protein purification system, ÄKTA™ start
Supplier: Cytiva
ÄKTA start is an easy to use chromatography system that automates manual purification procedures using, for example, HiTrap columns. It offers an entry level chromatography solution for the preparative purification of proteins at lab scale.
Expand 1 Items
Hydrophobic interaction chromatography media, Capto™ Phenyl (high sub)
Supplier: Cytiva
Capto™ Phenyl (high sub) is used in the capture and intermediate stages of protein purification.
Expand 1 Items
Affinity chromatography media, Glutathione Sepharose™ 4 Fast Flow
Supplier: Cytiva
Glutathione Sepharose 4 Fast Flow is used for the purification of GST-tagged proteins from bacterial lysates and other S-tranferases or glutathione-dependent proteins.
Expand 3 Items
Ion exchange chromatography media, Capto™ Q ImpRes
Supplier: Cytiva
Capto™ Q ImpRes is a strong anion exchange bioprocess medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective intermediate and polishing protein purification.
Expand 2 Items
Affinity chromatography media, IMAC Sepharose™ High Performance
Supplier: Cytiva
IMAC Sepharose High Performance media is easily charged by the user with the metalion of choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins.
Expand 1 Items
Accessories and spare parts for ÄKTApilot
Supplier: Cytiva
TC gasket, 25/4
Expand 12 Items
Ion exchange chromatography media, Capto™ DEAE
Supplier: Cytiva
Capto™ DEAE is a strong anion exchange bioprocessing medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective capture and intermediate protein purification when high volume throughput is essential.
Expand 2 Items
Gel filtration media, Sephacryl™ High Resolution
Supplier: Cytiva
Sephacryl™ High Resolution (HR) gel filtration media allow fast and reproducible purification of proteins, polysaccharides, and other macromolecules by gel filtration at laboratory and industrial scale.
Expand 10 Items
Protein G, MP Biomedicals
Supplier: MP Biomedicals
Protein G is a monomeric protein lacking cysteine residues. Recombinant Protein G is a highly stable surface receptor that is capable of binding the Fc portion of immunoglobulins, especially IgGs from a large number of species.
Expand 1 Items
Affinity purification media, GammaBind™ Plus Sepharose™
Supplier: Cytiva
GammaBind™ Plus Sepharose™ is GammaBind G, type 3, covalently immobilised to Sepharose™ CL-6B by malimide linkage.
Expand 3 Items
Capto™ MMC ImpRes multimodal chromatography media, Cytiva
Supplier: Cytiva
Capto MMC ImpRes can be used for initial or final polishing steps in MAb purification processes, as well as for polishing of antibody fragments such as domain antibodies (DAbs). The wide window of operation for conductivity and/or pH simplifies the process as conditioning after the protein A step can be omitted.
Expand 2 Items
Hydrophobic interaction chromatography media, Phenyl Sepharose™ High Performance
Supplier: Cytiva
Phenyl Sepharose™ High Performance is an aromatic hydrophobic interaction chromatography (HIC) medium, designed for intermediate and polishing step purification steps when high resolution has priority.
Expand 1 Items
Ion exchange chromatography media, DEAE Sepharose™ CL-6B
Supplier: Cytiva
DEAE Sepharose™ CL-6B is a weak anion exchanger.
Expand 1 Items
Affinity chromatography media, Ni Sepharose™ 6 Fast Flow
Supplier: Cytiva
Ni Sepharose 6 Fast Flow is a BioProcess medium that combines the advantages of Ni₂₊ for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform.
Expand 4 Items
Plasmid purification media, PlasmidSelect Xtra
Supplier: Cytiva
PlasmidSelect Xtra is a thiophilic aromatic adsorption chromatography medium with a selectivity that allows generic purification of supercoiled covalently closed circular forms of plasmid DNA to be separated from open circular forms.
Expand 1 Items
Phenyl Sepharose™ 6 Fast Flow (Low Sub) Hydrophobic Interaction Chromatography Media
Supplier: Cytiva
Phenyl Sepharose 6 Fast Flow is a well established, standard aromatic hydrophobic interaction chromatography (HIC) resin for capture and intermediate purification requiring low to resin hydrophobicity
Expand 2 Items
Affinity chromatography media, Dextrin Sepharose™ High Performance
Supplier: Cytiva
Dextrin Sepharose™ High Performance is an affinity media for purification of recombinant proteins tagged with Maltose Binding Protein (MBP).
Expand 2 Items
Gel filtration media, Sephadex™ G-25
Supplier: Cytiva
Sephadex™ G-25 is well established gel filtration medium for desalting and buffer exchange in industrial applications.
Expand 7 Items
Affinity chromatography media, Benzamidine Sepharose™ 4 Fast Flow (High Sub)
Supplier: Cytiva
Benzamidine Sepharose™ 4 Fast Flow (High Sub) is a tool for removal and/or purification of trypsin and trypsin-like serine proteases, as well as zymogens, including urokinase and prekallikrein.
Expand 1 Items
Affinity chromatography media, MabSelect™
Supplier: Cytiva
MabSelect™ is a protein A affinity resin designed for capturing monoclonal antibodies (mAbs) from large sample volumes.
Expand 2 Items
CM Sephadex™ C-50 Ion Exchange Chromatography Media, Cytiva
Supplier: Cytiva
Sephadex ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix.
Expand 2 Items
QAE Sephadex™ Ion Exchange Chromatography Media, Cytiva
Supplier: Cytiva
Sephadex ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix.
Expand 2 Items
SDS-PAGE Clean Up Kit
Supplier: Cytiva
SDS-PAGE clean up kit is designed to prepare samples for SDS-PAGE that are frequently difficult to analyse due to the presence of salts or low protein concentration.
Expand 1 Items
Ion exchange chromatography media, SOURCE™ 30Q
Supplier: Cytiva
SOURCE™ 30Q is a synthetic high performance, preparative, chromatography medium, based on a 30 µm monosized, rigid polystyrene/divinyl benzene polymer matrix.
Expand 1 Items
Sepharose™ CL-4B Phenyl Hydrophobic Interaction Chromatography Media
Supplier: Cytiva
Octyl-Sepharose™ CL-4B is a well-proven medium for hydrophobic interaction chromatography (HIC). Octyl immobilised on 4% cross-linked agarose.
Expand 1 Items
Affinity chromatography media, MabSelect™ PrismA
Supplier: Cytiva
MabSelect™ PrismA has been improved with an optimised high-flow agarose base matrix and a genetically engineered protein A-derived ligand, allowing future demands in mAb processing to be met.