You searched for: Protein Purification
Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.
Ion exchange chromatography media, Capto™ Q
Supplier: Cytiva
Capto™ Q is a strong anion exchange bioprocess medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective capture and intermediate protein purification when high volume throughput is essential.
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Capto™ MMC ImpRes multimodal chromatography media, Cytiva
Supplier: Cytiva
Capto MMC ImpRes can be used for initial or final polishing steps in MAb purification processes, as well as for polishing of antibody fragments such as domain antibodies (DAbs). The wide window of operation for conductivity and/or pH simplifies the process as conditioning after the protein A step can be omitted.
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Capto™ adhere ImpRes multimodal chromatography media, Cytiva
Supplier: Cytiva
Capto™ adhere ImpRes is a strong anion exchange multimodal BioProcess chromatography medium (resin), designed to meet the demands of modern large-scale manufacturers, for high-resolution polishing of monoclonal antibodies (MAbs) and other biomolecules. Capto™ adhere ImpRes is designed to allow effective MAb polishing in the second or third step of a purification scheme downstream of the protein A capture step. Polishing can be performed in either bind and elute (binding) or flowthrough (non binding) modes.
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Immobilised metal ion affinity chromatography (IMAC) media, Ni Sepharose™ excel
Supplier: Cytiva
Ni Sepharose™ excel is an IMAC medium pre-charged with nickel ions very strongly bound to a chelating ligand. Samples that usually cause stripping of metal ions can, therefore, be loaded to the medium. Ni Sepharose™ excel is designed primarily for capture and purification of histidine-tagged proteins secreted into cell culture supernatants from eukaryotic cells such as insect cells or CHO cells.
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Affinity chromatography media, HiTrap™ Capto™ Lentil Lectin
Supplier: Cytiva
Capto™ Lentil Lectin is an affinity chromatography resin for purification of glycoproteins and other molecules containing carbohydrates such as α-D-mannose and α-D-glucose or sterically related residues.
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Protease affinity chromatography media, PreScission™, Cytiva
Supplier: Cytiva
Enables the low-temperature cleavage of fusion proteins containing the PreScission Protease recognition sequence.
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Affinity chromatography media, IMAC Sepharose™ 6 Fast Flow
Supplier: Cytiva
IMAC Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix.
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Gel filtration media, Sephadex™ G-15
Supplier: Cytiva
Sephadex™ G-15 is a well established gel filtration medium for desalting and buffer exchange of peptides and small biomolecules >1500 molecular weight.
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Affinity chromatography media, IgG Sepharose™ 6 Fast Flow
Supplier: Cytiva
lgG Sepharose™ 6 Fast Flow, from Cytiva, is based on the rigid Sepharose 6 Fast Flow matrix, with human IgG covalently coupled to it.
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Ion exchange chromatography media, SP Sepharose™ High Performance
Supplier: Cytiva
SP Sepharose™ High Performance is composed of crosslinked agarose beads with a mean diameter of 34 µm, modified with sulphopropyl (SP) strong cation exchange groups.
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Ion exchange chromatography media, IEX Sepharose™ Fast Flow
Supplier: Cytiva
Q Sepharose Fast Flow is part of the Sepharose Fast Flow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades.
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Sepharose™ 4B Gel Filtration Base Matrix
Supplier: Cytiva
Sepharose™ 4B is an agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.
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Gel filtration media, Sephadex™ G-50
Supplier: Cytiva
Sephadex™ G-50 Medium is well established gel filtration medium for desalting and buffer exchange of biomolecules >30000 molecular weight. The larger bead size of G-50 Medium is suited for large scale separations.
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Sepharose™ 4 FF Octyl Fast Flow Hydrophobic Interaction Chromatography Media
Supplier: Cytiva
Octyl Sepharose™ 4 Fast Flow resin is based on cross-linked 4% agarose. The aliphatic ligand is immobilised to the base matrix with an ether linkage. The ligand contains no charged groups, making true hydrophobic interaction chromatography possible, without interfering ionic effects.
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Multimodal chromatography media, Capto™ Core 700
Supplier: Cytiva
Capto™ Core 700 is designed for intermediate purification and polishing of viruses and other large biomolecules (molecular weight >700000).
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Gelatin-Sepharose® 4B
Supplier: Cytiva
Gelatin Sepharose® 4B affinity media with immobilised gelatin is designed for the purification or depletion of fibronectin as gelatin binds to fibronectin with high specificity. Gelatin Sepharose® 4B is bovine gelatin coupled to Sepharose® 4B by the cyanogen bromide method.
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Affinity chromatography media, Streptavidin Sepharose™ High Performance
Supplier: Cytiva
Streptavidin Sepharose™ High Performance is an affinity chromatography medium designed for fast and reliable, high resolution purification of biotinylated biomolecules. Purified Streptavidin isolated from Streptomyces avidinii is immobilised on Sepharose™ High Performance.
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Multimodal chromatography media, Capto™ MMC
Supplier: Cytiva
Capto™ MMC is a multimodal cation exchanger with a different selectivity, compared with traditional ion exchangers.
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Gel filtration calibration kits
Supplier: Cytiva
Gel Filtration Calibration Kits are suitable for calibration of high-resolution gel filtration media and columns, such as Superdex, Superose and Sephacryl.
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Affinity chromatography media, MabSelect Xtra™
Supplier: Cytiva
MabSelect Xtra has the same recombinant Protein A ligand as MabSelect but is based on a high-flow agarose base matrix with increased porosity and a slightly decreased particle size compared to MabSelect.
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Gel filtration media, Sephadex™ G-10
Supplier: Cytiva
Sephadex™ G-10 is a well established gel filtration medium for desalting and buffer exchange of peptides and small biomolecules >700 molecular weight.
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Affinity chromatography media, Chelating Sepharose™ Fast Flow
Supplier: Cytiva
Chelating Sepharose Fast Flow is composed of cross-linked 6% agarose beads modified with iminodiacetic immobilized to the base matrix by stable ether linkages and sufficiently long spacer arms.
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Sepharose™ CL-4B Gel Filtration Base Matrix
Supplier: Cytiva
Sepharose™ CL-4B is a cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix.
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Gel filtration media, Sephadex™ LH-20
Supplier: Cytiva
Sephadex™ LH-20 is a liquid chromatography media for molecular sizing of natural product such as steroid, terpenoid, lipid and low molecular weight peptides. Sephadex™ LH-20 is pre-pared by hydroxypropylation of Sephadex™ G-25, a bead-formed dextran medium.
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Accessories and spare parts for ÄKTApilot
Supplier: Cytiva
TC gasket, 25/4
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SDS-PAGE Clean Up Kit
Supplier: Cytiva
SDS-PAGE clean up kit is designed to prepare samples for SDS-PAGE that are frequently difficult to analyse due to the presence of salts or low protein concentration.
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Superose™ 6 Gel Filtration Media, Prep Grade
Supplier: Cytiva
Superose™ 6 PG for separations of proteins and other biomolecules according to size. Wide separation in two molecular weight ranges from 1000 up to 5 million.
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Ion exchange chromatography media, Capto™ S ImpAct
Supplier: Cytiva
Capto™ S ImpAct is a strong cation exchange chromatography medium (resin) developed for polishing of monoclonal antibodies (MAbs) and other biomolecules.
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SP Sepharose™ XL Ion Exchange Chromatography Media
Supplier: Cytiva
SP Sepharose™ XL is a strong cation exchanger designed for use in the capture steps of biomolecules from clarified feed-stocks.
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Ion exchange chromatography media, SOURCE™ 15Q
Supplier: Cytiva
Mono-sized 15 um rigid beads gives low back pressure and high flow rates allowing for high productivity.