You searched for: Protein Purification

Supplier: Thermo Fisher Scientific

Pierce™ HisPur™ Cobalt Superflow Agarose is a tetradentate-chelating Superflow 6 support charged with divalent cobalt, designed for high-purity, large-scale purification of polyhistidine-tagged proteins.

Supplier: Thermo Fisher Scientific

The Pierce™ IgM Purification Kit is an easy to use affinity matrix and buffer system to purify IgM from mouse ascites fluid. The method uses immobilised mannan binding protein (MBP), which binds to IgM in the kit-supplied buffers.

Supplier: Thermo Fisher Scientific

HisPur™ Ni-NTA resin is a high-capacity, high-performance nickel-IMAC resin for routine affinity purification of His-tagged fusion proteins.

Supplier: Thermo Fisher Scientific

Pierce™ HisPur™ Ni-NTA Superflow Agarose is a nitrilotriacetic acid modified Superflow 6 support charged with divalent nickel (Ni⁺²) used for FPLC purification of poly-histidine-tagged proteins.

Supplier: Thermo Fisher Scientific

Pierce™ Monomeric Avidin Kit enables mild affinity purification of biotinylated proteins, peptides and other molecules. Avidin monomers have a much lower biotin-binding affinity than native tetrameric avidin enabling dissociation of biotinylated molecules using mild elution conditions.

Supplier: Thermo Fisher Scientific

Protein A UltraLink™ and Protein A Plus UltraLink™ resins are extremely durable beaded polyacrylamide supports to which IgG-binding Protein A has been immobilised for use in IgG purification methods. Protein A resin is used to bind and purify IgG in gravity-flow, spin-column or medium pressure chromatography methods.

Supplier: Thermo Fisher Scientific

Pierce™ chicken IgY purification kit purifies chicken antibodies from hen egg yolks using a unique delipidation reagent to separate protein from lipid and a precipitation reagent to selectively isolate the IgY fraction.

Supplier: Thermo Fisher Scientific

The Pierce™ NAb™ Spin Kits are convenient for rapid, small-scale affinity purification of antibodies from a variety of sample types. Each pre-filled microcentrifuge spin column of the immobilised protein resin enables quick purification of 100 to 1000 µg of IgG from 25 to 500 µl of serum or other sample. The actual amount of IgG purified varies depending upon the sample type and the specific spin column used.

Supplier: Thermo Fisher Scientific

Pierce™ Protein G UltraLink™ and Protein G Plus UltraLink™ resins are useful for purification of antibodies and immunoprecipitation (IP). Samples containing IgG are incubated with the resin-immobilised Protein G in a buffer that facilitates binding. After non-IgG and non-antigen components of the sample are washed from the resin, the bound IgG and antigen may be recovered by elution.

Supplier: Thermo Fisher Scientific

A solution of glutathione-S-transferase (≥100 units/mg).

Supplier: Thermo Fisher Scientific

SulfoLink™ coupling resin is porous, crosslinked, 6% beaded agarose that has been activated with iodoacetyl groups for covalent immobilisation of cysteine-peptides and other sulfhydryl molecules.

Supplier: Thermo Fisher Scientific

Pierce™ CDI-activated agarose is carbonyldiimidazole affinity chromatography resin, activated for covalent immobilisation of N-nucleophiles and primary amine ligands in aqueous or organic solvent conditions.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ High Select Top14 Abundant Protein Depletion Resin decreases the abundant protein and antibody components of human plasma samples, helping prepare the samples for mass spectrometry or 1D or 2D electrophoresis.

Supplier: Thermo Fisher Scientific

The Pierce™ Classic IP Kit uses protein A/G agarose beads, microcentrifuge spin columns and buffers to perform traditional immunoprecipitation assays.

Supplier: Thermo Fisher Scientific

Pierce™ protein A/G coated plate IP kit uses coated protein A/G microplates to perform immunoprecipitation assays in 96-well plates without resins, beads, centrifugation or magnets.

Supplier: Thermo Fisher Scientific

Immobilised GST is glutathione S-transferase from Schistosoma japonicum that has been covalently immobilised on crosslinked 6% beaded agarose.

Supplier: Thermo Fisher Scientific

Pierce™ c-Myc Tag IP/Co-IP Kit provides a simple and fast method to study c-Myc-tagged proteins, with advantages over the traditional IP procedure using protein A/G agarose.

Supplier: Thermo Fisher Scientific

Pierce™ High Sensitivity Streptavidin-HRP is a peroxidase-conjugated streptavidin biotin-binding protein that provides signal amplification like poly-HRP and exceptional storage stability.

Supplier: Thermo Fisher Scientific

The Pierce™ Crosslink Magnetic IP/Co-IP Kit uses crosslinking chemistry to covalently immobilise IP antibodies onto premium-quality protein A/G magnetic beads for effective immunoprecipitation and co-immunoprecipitation.

Supplier: Thermo Fisher Scientific

Jacalin is an alpha-D-galactose binding lectin extracted from jack-fruit seeds (Artocarpus integrifolia). This lectin is a glycoprotein (approx. 40 kDa) composed of four identical subunits. Jacalin binds to human IgA1 (also called serum or secretory IgA) with high specificity (i.e., it does not bind IgG or IgM). Thus, Jacalin that has been immobilised on crosslinked beaded agarose is useful for purifying human IgA from serum or colostrum samples.

Supplier: Thermo Fisher Scientific

UltraLink™ Hydrazide resin is used to prepare affinity columns with antibodies or other glycolsylated proteins.

Supplier: Thermo Fisher Scientific

CarboxyLink™ immobilisation kit is for covalent immobilisation of peptides or other carboxyl-containing (—COOH) molecules to a porous, beaded resin for use in affinity purification procedures.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ Maleimide Activated NeutrAvidin™ Protein is a specially-prepared form of avidin that decreases background in biotin-binding.

Supplier: Thermo Fisher Scientific

The Pierce™ Magnetic ChIP Kit provides a convenient method for efficient isolation of chromatin-bound DNA by immunoprecipitation for subsequent quantitation by PCR.

Supplier: Thermo Fisher Scientific

AminoLink™ coupling resin is crosslinked, 4% beaded agarose that has been activated with aldehyde groups to enable covalent immobilisation of antibodies and other proteins through primary amines.

Supplier: Thermo Fisher Scientific

The Pierce™ Protein Refolding Kit includes high-purity reagents and detailed instructions for using a matrix strategy to determine optimal buffer conditions for refolding recombinant proteins that have been denatured and solubilised from inclusion bodies.

Supplier: Thermo Fisher Scientific

Pierce™ Monomeric avidin agarose is immobilised onto 4% beaded agarose for purifying biotinylated proteins, peptides and other molecules.

Supplier: Thermo Fisher Scientific

Pierce™ High capacity streptavidin agarose consists of purified recombinant streptavidin that has been covalently immobilised at a high density onto high-quality crosslinked 6% beaded agarose.

Supplier: Thermo Fisher Scientific

The Melon™ Gel Spin Plate Kit for IgG Screening offers a high throughput format for quick purification and screening of antibodies.

Supplier: Thermo Fisher Scientific

AminoLink™ Immobilisation Kit contains AminoLink™ coupling resin and associated reagents sufficient to perform 5 protein immobilisations, with an expected yield of 2 to 20 mg protein per column.