You searched for: Enzyme Assays
Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.
Amplite® Fluorimetric Total NAD and NADH Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.
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Amplite® Fluorimetric Goat Anti-Mouse IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Horseradish Peroxidase (HRP) is a small molecule (MW ~40 Kd) that is widely used in a variety of biological detections.
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Amplite® Fluorimetric DPP4 Activity Assay Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® fluorimetric DPP4 activity assay kit provides a simple, quick, and direct protocol for measuring DPP4 Activity.
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Gaussia-firefly luciferase dual assay kit, Pierce™, Thermo Scientific
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Gaussia-Firefly Luciferase dual assay kit provides the necessary reagents to simultaneously detect intracellular Gaussia and red firefly luciferase activity in mammalian whole cell lysates.
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Amplite® Fluorimetric Goat Anti-Rabbit IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Horseradish Peroxidase (HRP) is a small molecule (MW ~40 KD) that is widely used in a variety of biological detections.
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Peroxide assay kit, PEROXsay™
Supplier: G-Biosciences
PEROXsay™ is a colorimetric quantitative peroxide assay that measures the oxidation of ferrous (Fe²⁺+) ions to ferric (Fe³⁺) ions. Peroxides react with a sugar alcohol converting it to a peroxyl radical that subsequently starts the oxidation of ferrous ions to ferric ions. The acidic pH of the assay allows the ferric (Fe³⁺) ion to complex with the colorimetric reagent, resulting in a change in absorbance that is proportional to the peroxide concentration.
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Amplite® Colorimetric Ammonia Quantitation Kit *Blue Color*
Supplier: AAT BIOQUEST
Ammonia is an important source of nitrogen for living systems.
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Amplite® Fluorimetric Aspartate Aminotransferase (AST) Assay Kit
Supplier: AAT BIOQUEST
Aspartate aminotransferase (AST), also called serum glutamic oxaloacetic transaminase (GOT), is a member of transferase family.
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Amplite® Colorimetric Glucose Oxidase Assay Kit
Supplier: AAT BIOQUEST
The glucose oxidase is a dimeric protein that catalyses the oxidation of beta-D-glucose into hydrogen peroxide and D-glucono-1,5-lactone, which is hydrolysed to gluconic acid.
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Amplite® Fluorimetric Aldehyde Quantitation Kit
Supplier: AAT BIOQUEST
The formation, reactivity and toxicity of aldehydes originating from the peroxidation of lipids of cellular membranes have received great attention in recent years.
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Amplite® Fluorimetric Protein Quantitation Kit *Orange Fluorescence*
Supplier: AAT BIOQUEST
Protein quantification is an essential task in protein purification, electrophoresis, cell biology, molecular biology and other research applications.
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Amplite® Colorimetric Zinc Ion Quantitation Kit
Supplier: AAT BIOQUEST
Zinc is an essential trace mineral element that plays an important role in a number of biological processes.
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Amplite® Colorimetric α-Ketoglutarate Quantitation Kit
Supplier: AAT BIOQUEST
Alpha-ketoglutarate (α-Ketoglutarate) is a key molecule in the Krebs cycle determining the overall rate of the citric acid cycle of the organism.
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MMP Red™ Substrate
Supplier: AAT BIOQUEST
The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.
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Amplite® Colorimetric Urea Quantitation Kit *Blue Color*
Supplier: AAT BIOQUEST
Urea is the final degradation product of protein and amino acid metabolism in animals.
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Amplite® Colorimetric Calcium Quantitation Kit *Blue Color*
Supplier: AAT BIOQUEST
Calcium is essential for all living organisms, particularly in cell physiology, where movement of the calcium ion Ca²⁺ into and out of the cytoplasm functions as a signal for many cellular processes.
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Amplite® Colorimetric Gama-Glutamyltransferase (GGT) Activity Assay Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® Colorimetric Gamma-Glutamyltransferase (GGT) Activity Assay Kit provides an efficient and straightforward method for measuring GGT activity across various sample types.
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Amplite® Fluorimetric Beta-Hydroxybutyrate (Ketone Body) Assay Kit
Supplier: AAT BIOQUEST
Ketone bodies are produced by the liver and used peripherally as an energy source when blood glucose levels drop.
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Amplite® Colorimetric Glucose and Sucrose Assay Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® Colorimetric Glucose and Sucrose Assay Kit offers a convenient method for measuring glucose and sucrose levels in various biological samples, such as serum, plasma, body fluids, food, tissue extracts, and growth medium.
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Amplite® Colorimetric Biotin Quantitation Kit
Supplier: AAT BIOQUEST
The avidin/streptavidin-biotin interaction is the strongest known non-covalent biological interaction (Kd = 10 to 15 M⁻¹) between a protein and its ligand.
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ReadiUse™ NADP Regenerating Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. AAT Bioquest's ReadiUse™ NADP regenerating kit provides a flexible prepackaged NADP regenerating system to accommodate various experimental designs.
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Amplite® Colorimetric Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Assay Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® colorimetric GAPDH activity assay kit is a sensitive and simple tool for monitoring GAPDH activity based on a coupled enzymatic reaction catalysed by GAPDH.
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Amplite® Fluorimetric Zinc Ion Quantitation Kit
Supplier: AAT BIOQUEST
Zinc is an essential trace mineral element that plays an important role in a number of biological processes.
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Pierce™ Cypridina Luciferase Glow Assay Kit
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Cypridina Luciferase Glow Assay Kit provides an extremely bright and stable bioluminescence signal (half-life greater than 1 hour) in the presence of Cypridina luciferase reporters.
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Amplite® Fluorimetric Total Thiol Quantitation Assay Kit *Green Fluorescence*
Supplier: AAT BIOQUEST
The detection and measurement of free thiol (such as free cysteine, glutathione and cysteine residues in proteins) is one of the essential tasks for investigating biological processes and events in many biological systems.
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Amplite® Rapid Fluorimetric Total Thiol Quantitation Assay Kit *Green Fluorescence*
Supplier: AAT BIOQUEST
The detection and measurement of free thiol (such as free cysteine, glutathione and cysteine residues in proteins) is one of the essential tasks for investigating biological processes and events in many biological systems.
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Amplite® Rapid Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence*
Supplier: AAT BIOQUEST
When cells are exposed to increased levels of oxidative stress, GSSG will accumulate and the ratio of GSH to GSSG will decrease.
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Beadlite™ Rapid Colorimetric Thiol Quantitation Kit for Nanoparticles
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. Beadlite™ Rapid Colorimetric Thiol Quantitation kit provides a robust method to quantify thiol-functionalised nanoparticle with our proprietary Thiolite™ 490 that has its maximum absorbance at ~490 nm.
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Amplite® Fluorimetric Peroxynitrite Quantification Kit *Green Fluorescence*
Supplier: AAT BIOQUEST
Peroxynitrite (ONOO-) is a strong oxidizing species and a highly active nitrating agent.
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Amplite® Fluorimetric Endotoxin Detection Kit
Supplier: AAT BIOQUEST
Lipopolysaccharide (LPS), also known as endotoxin, is the major component of the outer membrane of Gram-negative bacteria.
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