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You searched for: Enzyme Assays

Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.

Amplite® Fluorimetric Peroxynitrite Quantification Kit *Green Fluorescence*

Amplite® Fluorimetric Peroxynitrite Quantification Kit *Green Fluorescence*

Supplier: AAT BIOQUEST

Peroxynitrite (ONOO-) is a strong oxidizing species and a highly active nitrating agent.

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Amplite® Fluorimetric Endotoxin Detection Kit

Amplite® Fluorimetric Endotoxin Detection Kit

Supplier: AAT BIOQUEST

Lipopolysaccharide (LPS), also known as endotoxin, is the major component of the outer membrane of Gram-negative bacteria.

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ReadiUse™ NADPH Regenerating Kit

ReadiUse™ NADPH Regenerating Kit

Supplier: AAT BIOQUEST

NADPH provides the reducing equivalents for biosynthetic reactions and for oxidation-reduction involved in protection against the toxicity of ROS (reactive oxygen species).

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Amplite® Fluorimetric Lead (II) Ion Quantitation Kit

Amplite® Fluorimetric Lead (II) Ion Quantitation Kit

Supplier: AAT BIOQUEST

Lead is a highly poisonous metal to human beings, especially young children.

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Amplite® Fluorimetric Total NADP and NADPH Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Total NADP and NADPH Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

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Amplite® Fluorimetric Glutathione Assay Kit *Green Fluorescence*

Amplite® Fluorimetric Glutathione Assay Kit *Green Fluorescence*

Supplier: AAT BIOQUEST

The monitoring of reduced and oxidised glutathione (GSH) in biological samples is essential for evaluating the redox and detoxification status of cells and tissues in relation to the protective role of glutathione against oxidative and free-radical-mediated cell injury.

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Amplite® Fluorimetric Ascorbic Acid Assay Kit

Amplite® Fluorimetric Ascorbic Acid Assay Kit

Supplier: AAT BIOQUEST

L-Ascorbic Acid (also called Vitamin C) is a critical metabolite for both plant and animals in cell division, growth and defense.

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Amplite® Fluorimetric Butyrylcholinesterase Activity Assay Kit

Amplite® Fluorimetric Butyrylcholinesterase Activity Assay Kit

Supplier: AAT BIOQUEST

Butyrylcholinesterase (EC 3.1.1.8; BChE), also known as pseudocholinesterase or plasma cholinesterase, is mainly synthesized liver and present in blood.

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Amplite® Fluorimetric Myeloperoxidase Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Myeloperoxidase Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Myeloperoxidase (MPO), most abundantly present in neutrophils and monocytes, is a green hemoprotein having peroxidase activity.

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Gaussia luciferase glow assay kit, Pierce™

Gaussia luciferase glow assay kit, Pierce™

Supplier: Thermo Fisher Scientific

The Thermo Scientific Pierce Gaussia Luciferase Glow Assay kit provides an extremely bright and stable bioluminescence signal (half-life greater than one hour), especially in the presence of Gaussia-Dura luciferase reporter.

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Amplite® Universal Fluorimetric Protease Activity Assay Kit *Green Fluorescence*

Amplite® Universal Fluorimetric Protease Activity Assay Kit *Green Fluorescence*

Supplier: AAT BIOQUEST

Monitoring of various protease activities has become a routine task for many biological laboratories.

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Amplite® Colorimetric NADP/NADPH Ratio Assay Kit

Amplite® Colorimetric NADP/NADPH Ratio Assay Kit

Supplier: AAT BIOQUEST

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

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Amplite® Fluorimetric Monoamine Oxidase Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Monoamine Oxidase Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Monoamine oxidases (MAO) are a family of flavin-containing amine oxidoreductases that catalyse the oxidation of monoamines.

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Amplite® Fluorimetric Formaldehyde Quantitation Kit *Green Fluorescence*

Amplite® Fluorimetric Formaldehyde Quantitation Kit *Green Fluorescence*

Supplier: AAT BIOQUEST

Formaldehyde is a naturally occurring substance.

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Yeast β-galactosidase assay kit, Pierce™

Yeast β-galactosidase assay kit, Pierce™

Supplier: Thermo Fisher Scientific

Yeast β-Galactosidase Assay Kit is specially formulated for yeast cells and ideal for identifying protein interactions in vivo using two-hybrid systems.

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Amplite® Fluorimetric Glutamate Oxidase Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Glutamate Oxidase Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Glutamate oxidase belongs to the family of oxidoreductases, specifically those acting on the CH-NH₂ group of donors with oxygen as an acceptor.

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Amplite® Fluorimetric Hypochlorite (Hypochlorous Acid) Assay Kit

Amplite® Fluorimetric Hypochlorite (Hypochlorous Acid) Assay Kit

Supplier: AAT BIOQUEST

Hypochlorite anion (ClO-) and its protonated form, hypochlorous acid (HClO) are critical reactive oxygen species (ROS) in biological systems.

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Amplite® Fluorimetric Neutrophil Elastase Activity Assay Kit

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. The Amplite® fluorimetric neutrophil elastase activity assay kit provides a robust and accurate method for quantifying neutrophil elastase (NE) activity.

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Amplite® Fluorimetric DPP4 Inhibitor Screening Kit

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. The Amplite® Fluorimetric DPP4 Inhibitor Screening Kit provides an efficient and direct method for evaluating potential DPP4 inhibitors.

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Pierce™ Alkaline Phosphatase, Biotinylated

Pierce™ Alkaline Phosphatase, Biotinylated

Supplier: Thermo Fisher Scientific

Biotinylated alkaline phosphatase is most commonly used in immunohistochemistry (IHC) to amplify the signal of biotinylated primary antibodies using the ABC staining method.

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Amplite® Fluorimetric Hydrogen Peroxide Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Hydrogen Peroxide Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Hydrogen peroxide (H₂O₂) is a reactive oxygen metabolic by-product that serves as a key regulator for a number of oxidative stress-related states.

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Amplite® Fluorimetric Total NAD and NADH Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Total NAD and NADH Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

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Amplite® Colorimetric Beta-Hydroxybutyrate (Ketone Body) Assay Kit

Amplite® Colorimetric Beta-Hydroxybutyrate (Ketone Body) Assay Kit

Supplier: AAT BIOQUEST

Ketone bodies are produced by the liver and used peripherally as an energy source when blood glucose levels drop.

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Amplite® Colorimetric Peroxidase (HRP) Assay Kit *Blue Color*

Amplite® Colorimetric Peroxidase (HRP) Assay Kit *Blue Color*

Supplier: AAT BIOQUEST

Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody in a 4:1 ratio.

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Amplite® Fluorimetric Sphingomyelinase Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Sphingomyelinase Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Five types of sphingomyelinase (SMase) have been identified based on their cation dependence and pH optima of action.

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Amplite® Fluorimetric NADH Assay Kit *Red Fluorescence*

Amplite® Fluorimetric NADH Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

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Amplite® Rapid Colorimetric Maleimide Quantitation Kit

Amplite® Rapid Colorimetric Maleimide Quantitation Kit

Supplier: AAT BIOQUEST

A variety of crosslinking reagents with a maleimide group are widely used for crosslinking proteins to proteins, or proteins to other biomolecules.

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Amplite® Colorimetric Hypochlorite (Hypochlorous Acid) Assay Kit

Amplite® Colorimetric Hypochlorite (Hypochlorous Acid) Assay Kit

Supplier: AAT BIOQUEST

Hypochlorite anion (ClO-) and its protonated form, hypochlorous acid (HClO) are critical reactive oxygen species (ROS) in biological systems.

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Amplite® Colorimetric Biotin Quantitation Kit

Amplite® Colorimetric Biotin Quantitation Kit

Supplier: AAT BIOQUEST

The avidin/streptavidin-biotin interaction is the strongest known non-covalent biological interaction (Kd = 10 to 15 M⁻¹) between a protein and its ligand.

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Amplite® Human Serum Albumin (HSA) Site I Binding Assay Kit

Amplite® Human Serum Albumin (HSA) Site I Binding Assay Kit

Supplier: AAT BIOQUEST

Human serum albumin (HSA) is one of the most important carriers for acidic drugs in human plasma and has been shown to bind a large number of different compounds in a reversible manner.

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