You searched for: Enzyme Assays
Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.
Amplite® Colorimetric Pyruvate Assay Kit
Supplier: AAT BIOQUEST
Pyruvate is an important chemical compound in intracellular metabolic pathways.
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Amplite® Colorimetric Hydrogen Peroxide Assay Kit
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Hydrogen peroxide (H2O2) is a reactive oxygen metabolic by-product that serves as a key regulator for a number of oxidative stress-related states.
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Amplite® Fluorimetric Glucose Quantitation Kit
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Glucose, a monosaccharide, is the most important carbohydrate in biology.
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Amplite® Fluorimetric Glycerol 3-Phosphate (G3P) Assay Kit
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Glycerol 3-Phosphate (G3P) is an important intermediate in the glycolysis metabolic pathway.
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Amplite® Rapid Colorimetric Total Protein Thiol Quantitation Assay Kit
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Protein thiols are very important to protein structure, protein function and biological system redox environment.
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Amplite® Colorimetric L-Aspartate (Aspartic Acid) Assay Kit
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Aspartate (or Aspartic acid) is a negatively charged, polar amino acid.
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Amplite® Colorimetric Glycerol 3-Phosphate (G3P) Assay Kit
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Glycerol 3-Phosphate (G3P) is an important intermediate in glycolysis metabolic pathway.
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Amplite® Luciferase Reporter Gene Assay Kit *Bright Glow*
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Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.
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Amplite® Total Sulfide Quantification Kit
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Hydrogen sulfide is not usually a health risk at concentrations present in household water.
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Amplite® Colorimetric L-Alanine Assay Kit
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L-alanine (L-Ala) plays a crucial role as a building block of important proteins.
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Amplite® Universal Fluorimetric MMP Activity Assay Kit *Red Fluorescence*
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The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.
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MMP Green™ Substrate
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The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.
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Amplite® IR
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Our Amplite® IR is a fluorogenic peroxidase substrate that generates near infrared fluorescence upon reaction with peroxidase and H2O2.
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Amplite® Fluorimetric Beta-Galactosidase Assay Kit *Green Fluorescence*
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E. coli beta-galactosidase is a 464 Kd tetramer.
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Amplite® Colorimetric Maleimide Quantitation Kit
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Maleimides can be directly assayed spectrophotometrically at 302 nm.
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Amplite® Universal Fluorimetric Kinase Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
The Amplite® Universal Kinase Assay Kit is based on the monitoring of ADP formation, which is directly proportional to enzyme phoshphotransferase activity and is measured fluorimetricaly. This kit provides a fast, simple, and homogeneous assay for measure kinases activities. The characteristics of its high sensitivity (<0.2 uM ADP), broad ATP tolerance (1-300 uM), non-antibody based, non-radioactive and no-wash method to detect the amount of ADP produced as a result of enzyme activity make it an ideal kit for determining kinase Michaelis-Menten kinetics and for screening and identifying kinase inhibitors. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation with no separation steps required.
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Amplite® Fluorimetric NADP/NADPH Ratio Assay Kit *Red Fluorescence*
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Nicotinamide adenine dinucleotide (NAD⁺) and nicotinamide adenine dinucleotide phosphate (NADP⁺) are two important cofactors found in cells.
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Amplite® Fluorimetric Total NADP and NADPH Assay Kit *Red Fluorescence*
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Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.
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Amplite® Fluorimetric DPP4 Activity Assay Kit
Supplier: AAT BIOQUEST
Designed for consistent and dependable results. The Amplite® fluorimetric DPP4 activity assay kit provides a simple, quick, and direct protocol for measuring DPP4 Activity.
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Amplite® Fluorimetric Myeloperoxidase Assay Kit *Red Fluorescence*
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Myeloperoxidase (MPO), most abundantly present in neutrophils and monocytes, is a green hemoprotein having peroxidase activity.
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Amplite® Fluorimetric Monoamine Oxidase Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Monoamine oxidases (MAO) are a family of flavin-containing amine oxidoreductases that catalyse the oxidation of monoamines.
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Amplite® Fluorimetric Glucose Oxidase Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
The glucose oxidase is a dimeric protein that catalyses the oxidation of beta-D-glucose into hydrogen peroxide and D-glucono-1,5-lactone, which is hydrolyzed to gluconic acid.
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Amplite® Fluorimetric Oxaloacetate Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Oxaloacetate is an important part of citric acid cycle, where it reacts with Acetyl-CoA to form citrate.
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Amplite® Fluorimetric Melanin Assay Kit
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Melanins have very diverse roles and functions in various organisms.
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Amplite® Fluorimetric Malondialdehyde (MDA) Quantitation Kit
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Malondialdehyde (MDA) is one of the natural byproducts during lipid peroxidation.
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Amplite® Colorimetric Endotoxin Detection Kit
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Lipopolysaccharide (LPS), also known as endotoxin, is the major component of the outer membranes of Gram-negative bacteria.
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Amplite® Fluorimetric Aspartate Aminotransferase (AST) Assay Kit
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Aspartate aminotransferase (AST), also called serum glutamic oxaloacetic transaminase (GOT), is a member of transferase family.
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Amplite® Colorimetric Glucose Oxidase Assay Kit
Supplier: AAT BIOQUEST
The glucose oxidase is a dimeric protein that catalyses the oxidation of beta-D-glucose into hydrogen peroxide and D-glucono-1,5-lactone, which is hydrolysed to gluconic acid.
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Amplite® Colorimetric Peroxidase (HRP) Assay Kit *Blue Color*
Supplier: AAT BIOQUEST
Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody in a 4:1 ratio.
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Amplite® Fluorimetric Sphingomyelinase Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Five types of sphingomyelinase (SMase) have been identified based on their cation dependence and pH optima of action.
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