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You searched for: Enzyme Assays

Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.

Proteasome 20S Assay Kit

Supplier: ENZO LIFE SCIENCES

A QuantiZyme™ Assay System. Fluorogenic, non-radioactive assay designed to measure chymotrypsin-like protease activity of purified 20S proteasome. This 96-well assay is useful for screening inhibitors and modulators of the 20S proteasome.

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Fluorometric polymerase activity assay kit, EvaEZ™

Supplier: Biotium

EvaEZ™ Fluorometric Polymerase Activity Assay Kit provides an easy and accurate way to determine the activity of a nucleic acid polymerase without using radioisotopes.

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Matrix Metalloproteinase-14 (MMP-14) colorimetric drug discovery kit

Supplier: ENZO LIFE SCIENCES

A QuantiZyme™ Assay System. The MMP-14 Colorimetric Drug Discovery Kit is a complete assay sytem designed to screen for inhibitors of matrix metalloproteinase-14 (MMP-14, membrane-type matrix metalloproteinase 1, MT1-MMP) using a thiopeptide as a chromogenic substrate.

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Matrix Metalloproteinase-12 (MMP-12) colorimetric drug discovery kit

Supplier: ENZO LIFE SCIENCES

A QuantiZyme™ Assay System. The MMP-12 Colorimetric Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-12 (MMP-12, macrophage elastase, metalloelastase) using a thiopeptide as a chromogenic substrate. The assays are performed in a convenient 96-well plate format. Included are active enzyme, assay buffer, a prototypic control inhibitor (NNGH), and a detailed instruction booklet.

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Cathepsin S drug discovery kit

Supplier: ENZO LIFE SCIENCES

A QuantiZyme™ Assay System. The Cathepsin S Drug Discovery Kit is a complete assay system designed to screen inhibitors of cathepsin S. Using a convenient microplate format, cathepsin S activity is monitored fluorimetrically using the substrate provided in the kit.

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DPPIV Drug discovery kit

Supplier: ENZO LIFE SCIENCES

The DPPIV Drug Discovery Kit is a complete assay system designed to screen DPPIV inhibitors, a potential therapeutic target, providing enough material to perform at least 96 assays.

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Fluorometric drug discovery assay kit, SIRT5, Fluor-de-Lys®

Fluorometric drug discovery assay kit, SIRT5, Fluor-de-Lys®

Supplier: ENZO LIFE SCIENCES

The SIRT5 Fluorometric Drug Discovery Kit is a sensitive fluorometric assay is designed to measure the lysyl desuccinylase activity of the recombinant human SIRT5.

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Glutathione peroxidase activity kit

Supplier: ENZO LIFE SCIENCES

The Glutathione Peroxidase activity kit is a convenient 96-well plate based assay for the higher throughput analysis of glutathione peroxidase activity in a variety of sample matrices in just 30 minutes.

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Fluorometric drug discovery assay kit, Green SIRT5, Fluor-de-Lys®

Fluorometric drug discovery assay kit, Green SIRT5, Fluor-de-Lys®

Supplier: ENZO LIFE SCIENCES

FLUOR DE LYS® Green SIRT5 fluorometric drug discovery kit is a sensitive fluorometric assay that measures the lysyl desuccinylase activity of the recombinant human SIRT5.

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Protease assay kit

Protease assay kit

Supplier: G-Biosciences

This Protease Assay Kit is designed for the quantitative determination of proteases present in protein sample, using a dye-labelled protein substrate. The proteases present in the sample of interest will digest the protein substrate and release dye labelled peptides. The absorbance of the dye-labelled peptide is measured at 570 nm for determination of protease activity.

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Protease and peptidase substrate

Supplier: Biotium

Fluorogenic substrates for proteases and peptidases.

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Matrix Metalloproteinase-13 (MMP-13) colorimetric drug discovery kit

Supplier: ENZO LIFE SCIENCES

A QuantiZyme™ Assay System. The MMP-13 Colorimetric Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-13 (MMP-13, collagenase-3) using a thiopeptide as a chromogenic substrate.

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Neutrophil Elastase colorimetric drug discovery kit

Neutrophil Elastase colorimetric drug discovery kit

Supplier: ENZO LIFE SCIENCES

Neutrophil Elastase Colorimetric Drug Discovery Kit is a complete assay system designed to screen inhibitors of neutrophil elastase, providing enough material to perform at least 96 assays.

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MMP Fluorometric drug discovery kits, RED and GREEN

Supplier: ENZO LIFE SCIENCES

The matrix metalloproteinase (MMP) RED and GREEN Drug Discovery Kits are complete assay systems designed to screen MMP inhibitors, using long-wavelength quenched fluorogenic substrates OmniMMP™ RED or Fluorogenic Substrate (GREEN). The assays are performed in a convenient 96-well microplate format, with the compound NNGH also included as a prototypic control inhibitor.

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Granzyme B assay kit for drug discovery

Supplier: ENZO LIFE SCIENCES

The Granzyme B Assay Kit for Drug Discovery is a complete assay system designed to measure the protease activity of granzyme B. It contains a colorimetric substrate (IEPD-pNA) to measure this activity; cleavage of the p-nitroanilide (pNA) group from the colorimetric substrate increases absorption at 405 nm.

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Matrix Metalloproteinase-2 (MMP-2) colorimetric drug discovery kit

Supplier: ENZO LIFE SCIENCES

A QuantiZyme™ Assay System. The MMP-2 Colorimetric Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-2 (MMP-2, gelatinase A) using a thiopeptide as a chromogenic substrate. The assays are performed in a convenient 96-well microplate format. The kit is useful to screen inhibitors of MMP-2, a potential therapeutic target.

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Phosphatase assay kit, BIOMOL® Green

Phosphatase assay kit, BIOMOL® Green

Supplier: ENZO LIFE SCIENCES

BIOMOL® Green provides a simple and convenient method for colorimetric phosphate quantitation. It is suitable for applications include assays for phospholipid phosphatases, tyrosyl-tRNA synthetase (coupled with pyrophosphatase) and a viral RNA triphosphatase. It is most widely applied to protein phosphatase assays.

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PKA kinase activity kit

Supplier: ENZO LIFE SCIENCES

The PKA Kinase activity kit is an efficient assay that utilizes little sample volume. This kit gives you the ability to an end-point or kinetic assay read-out in a convenient 96-well plate based assay and offers easy sample handling protocols. The assay also offers a high signal to background ratio.

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Matrix Metalloproteinase-20 (MMP-20) colorimetric drug discovery kit

Supplier: ENZO LIFE SCIENCES

A QuantiZyme™ Assay System. The MMP-20 Colorimetric Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-20 (MMP-20, enamelysin) using a thiopeptide as a chromogenic substrate.

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Matrix Metalloproteinase-1 (MMP-1) colorimetric drug discovery kit

Matrix Metalloproteinase-1 (MMP-1) colorimetric drug discovery kit

Supplier: ENZO LIFE SCIENCES

A QuantiZyme™ Assay System. The MMP-1 Colorimetric Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-1 (MMP-1, interstitial collagenase, fibroblast collagenase) using a thiopeptide as a chromogenic substrate.

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PKC kinase activity kit

Supplier: ENZO LIFE SCIENCES

The PKC Kinase activity kit is an efficient assay that utilizes little sample volume. This kit gives you the ability to an end-point or kinetic assay read-out in a convenient 96-well plate based assay and offers easy sample handling protocols. The assay also offers a high signal to background ratio.

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Matrix Metalloproteinase-12 (MMP-12) fluorometric drug discovery kit

Supplier: ENZO LIFE SCIENCES

A QuantiZyme™ Assay System. The MMP-12 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-12 (MMP-12, metalloelastase, macrophage elastase) using a quenched fluorogenic peptide: OmniMMP™ fluorogenic substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH.

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Protease Estimation Assay Kit, ProteSEEKER™

Protease Estimation Assay Kit, ProteSEEKER™

Supplier: G-Biosciences

ProteSEEKER™ identifies specific types of proteases with a panel of twelve protease inhibitors and a sensitive colorimetric protease screening assay. ProteSEEKER™ allows researchers to screen their protein samples and establish which specific class of proteases are present and therefore design a highly specific protease inhibitor cocktail using the minimal number of protease inhibitors. Alternatively, ProteSEEKER™ can be used to test existing protease inhibitor cocktails and identify their inadequacies and therefore supplement in additional protease inhibitors.

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ProteoStat™ PDI assay kit

Supplier: ENZO LIFE SCIENCES

The PROTEOSTAT® PDI assay kit provides a simple, homogenous assay for screening modulators of PDI enzymatic activity. This is accomplished by monitoring PDI-catalyzed reduction of insulin in the presence of Dithiothreitol (DTT), resulting in the formation of insulin aggregates which then bind avidly to the red-emitting fluorogenic PROTEOSTAT® PDI detection dye.

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Matrix Metalloproteinase-1 (MMP-1) fluorometric drug discovery kit

Supplier: ENZO LIFE SCIENCES

A QuantiZyme™ Assay System. The MMP-1 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-1 (MMP-1, interstitial collagenase) using a quenched fluorogenic peptide: OmniMMP™ fluorogenic substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties.

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AEC Peroxidase substrate kit

Supplier: ENZO LIFE SCIENCES

The AEC Peroxidase Substrate Kit contains reagents for visualization of peroxidase reactions using Aminoethylcarbazole (AEC). In the presence of H2O2, peroxidases catalyze the oxidation of AEC into a brick red precipitate. The supplied reagents are sufficient for preparation of 300 ml of ready-to-use color reaction mixture.

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Matrix Metalloproteinase-3 (MMP-3) fluorometric drug discovery kit

Supplier: ENZO LIFE SCIENCES

A QuantiZyme™ Assay System. The MMP-3 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-3 (MMP-3, stromelysin-1) using a quenched fluorogenic peptide: OmniMMP™ fluorogenic substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties.

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Fluorescent protease assay, Fluoro™

Fluorescent protease assay, Fluoro™

Supplier: G-Biosciences

The Fluoro™ Protease Assay Kit is designed for the quantitative determination of proteases present in a protein sample. The assay uses fluorescein isothiocyanate (FITC)-labelled casein as a general protease substrate. The fluorescein label on the FITC-casein is highly quenched. When the proteases present in the sample of interest digest the FITC-casein substrate into smaller peptides, the quenching of the fluorescence label is relieved and the fluorescence of the substrate is increased. The fluorescence of the FITC-labeled peptide is measured with excitation at 485 nm and emission at 530 nm to determine protease activity. The kit detects picogram level of proteases present in the sample.

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Matrix Metalloproteinase-9 (MMP-9) fluorometric drug discovery kit

Supplier: ENZO LIFE SCIENCES

A QuantiZyme™ Assay SystemThe MMP-9 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-9 (MMP-9, gelatinase B) using a quenched fluorogenic peptide: OmniMMP™ fluorogenic substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties.

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Matrix Metalloproteinase-8 (MMP-8) fluorometric drug discovery kit

Supplier: ENZO LIFE SCIENCES

A QuantiZyme™ Assay System. The MMP-8 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-8 (MMP-8, neutrophil collagenase, collagenase-2) using a quenched fluorogenic peptide: OmniMMP™ fluorogenic substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties

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