Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.
ReadiLink™ Rapid mFluor™ Violet 420 Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)
Supplier: AAT BIOQUEST
AAT Bioquest's mFluor™ dyes are developed for flow cytometry-focused applications.
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ReadiLink™ Rapid mFluor™ Violet 540 Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)
Supplier: AAT BIOQUEST
AAT Bioquest's mFluor™ dyes are developed for flow cytometry-focused applications.
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Avidin, horseradish peroxidase (HRP) conjugated, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ HRP conjugated avidin is used in biotin-based detection methods, including Western blotting or ELISA detection with appropriate enzyme substrates.
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Cell Explorer™ Live Cell Tracking Kit
Supplier: AAT BIOQUEST
The kit uses a weakly fluorescent dye that carries a cell-retaining moiety.
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Dye labeling kit (FITC), HOOK™
Supplier: G-Biosciences
FITC (fluorescein isothiocyanate) is a commonly used fluorescent label for proteins, as it contains the groups required for conjugating to amino, sulfhydryl, imidazoyl, tyrosyl or carbonyl groups of proteins. FITC has a molecular weight of 389, and excitation and emission wavelengths of 494 nm and 520 nm, respectively, therefore emitting green visible light.
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Buccutite™ Rapid PerCP Antibody Labelling Kit
Supplier: AAT BIOQUEST
Production scale optimised for labelling 1 mg antibody per reaction. Buccutite™ Rapid PerCP antibody labelling kits, designed for large-scale production, provide a streamlined approach for labelling antibodies with PerCP, APC, PE and iFluor® tandem dyes.
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ReadiLink™ Rapid iFluor® Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)
Supplier: AAT BIOQUEST
Although FITC is still the most popular fluorescent labelling dye for preparing green fluorescent bioconjugates, there are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence.
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Buccutite™ Rapid PE-Cy5 Tandem Antibody Labelling Kit
Supplier: AAT BIOQUEST
Production scale optimised for labelling 1 mg antibody per reaction. Buccutite™ Rapid PE-Cy5 tandem antibody labelling kits, designed for large-scale production, provide a streamlined approach for labelling antibodies with PE, APC, PerCP and iFluor® tandem dyes.
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ReadiLink™ xtra Rapid XFD488 Antibody Labelling Kit *BSA-Compatible, XFD488 Same Structure to Alexa Fluor™ 488* (2 Labelings)
Supplier: AAT BIOQUEST
XFD488 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 488 (Alexa Fluor® is the trademark of ThermoFisher).
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ReadiLink™ Cy5 Nick Translation dsDNA Labelling Kit
Supplier: AAT BIOQUEST
It is compatible with a wide variety of sample materials, including bacterial artificial chromosome (BAC) DNA, human genomic DNA, purified PCR products, supercoiled and linearized plasmid DNA.
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ReadiLink™ Cy3 Nick Translation dsDNA Labelling Kit
Supplier: AAT BIOQUEST
It is compatible with a wide variety of sample materials, including bacterial artificial chromosome (BAC) DNA, human genomic DNA, purified PCR products, supercoiled and linearised plasmid DNA.
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CytoWatch™ Wash-free Fluorescence Cell Imaging Buffer, 10X
Supplier: AAT BIOQUEST
CytoWatch™ Wash-free fluorescence cell imaging buffer *10X* is a ready-to-use buffer optimised for fluorescence cell imaging.
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Carboxyl reactive biotinylation reagents, EZ-Link™
Supplier: Thermo Fisher Scientific
Carboxyl groups, in the form of carboxy termini, aspartate residues or glutamate residues, can be targeted for biotin labeling using amine-derivatised biotin molecules. This reaction is mediated by a class of crosslinkers known as carbodiimides and results in the formation of an amide bond. The reaction with EDC, the most common carbodiimide crosslinker, is generally performed in an MES buffer at pH 4,5 to 5 and requires just minutes to complete.
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PSA™ iFluor® 350 Imaging Kit with Goat Anti-Rabbit IgG
Supplier: AAT BIOQUEST
iFluor® 350 PSA kit is a much superior replacement for Alexa Fluor 350 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.
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PSA™ iFluor® 350 Imaging Kit with Goat Anti-Mouse IgG
Supplier: AAT BIOQUEST
iFluor® 350 PSA kit is a much superior replacement for Alexa Fluor 350 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.
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PSA™ iFluor® 488 Imaging Kit with Goat Anti-Mouse IgG
Supplier: AAT BIOQUEST
iFluor® 488 PSA kit is a much superior replacement for Alexa Fluor 488 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.
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ReadiLink™ iFluor® 555 FISH Fluorescence Imaging Kit
Supplier: AAT BIOQUEST
The kit provides Taq DNA polymerase enzyme which incorporates iFluor® 555-dUTPs in the target DNA through Polymerase Chain Reaction (PCR).
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Buccutite™ Rapid PE Antibody Labelling Kit
Supplier: AAT BIOQUEST
Production scale optimised for labelling 1 mg antibody per reaction. Buccutite™ Rapid PE antibody labelling kits, designed for large-scale production, offer a convenient and efficient method to label antibodies with PE, APC and iFluor® tandem dyes.
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Buccutite™ Rapid PE-Cy7 Tandem Antibody Labelling Kit
Supplier: AAT BIOQUEST
Production scale optimised for labelling 1 mg antibody per reaction. Buccutite™ Rapid PE-Cy7 tandem antibody labelling kits, designed for large-scale production, provide a streamlined approach for labelling antibodies with PE, APC, PerCP and iFluor® tandem dyes.
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PEGylated BM (bismaleimido) Crosslinkers, Homobifunctional, Pierce™
Supplier: Thermo Fisher Scientific
Cross linkers are used for the identification of near-neighbour protein relationships and ligand-receptor interactions. Homobifunctional, amine-reactive NHS esters or imidates, and heterobifunctional, amine-reactive, photoactivatable phenyl azides are the most commonly used cross linkers for these applications.
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ReadiLink™ iFluor® 488 Nick Translation dsDNA Labeling Kit
Supplier: AAT BIOQUEST
It is compatible with a wide variety of sample materials, including bacterial artificial chromosome (BAC) DNA, human genomic DNA, purified PCR products, supercoiled and linearized plasmid DNA.
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Buccutite™ Poly-HRP Antibody Conjugation Kit
Supplier: AAT BIOQUEST
Poly-HRP-antibody conjugates is considered to have the highest sensitivity in immunoassays where the sample is limited, or a target molecule is present at extremely low level.
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ReadiLink™ xtra Rapid XFD555 Antibody Labelling Kit *BSA-Compatible, XFD555 Same Structure to Alexa Fluor™ 555* (2 Labellings)
Supplier: AAT BIOQUEST
XFD555 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 555 (Alexa Fluor® is the trademark of ThermoFisher).
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ReadiLink™ xtra Rapid XFD594 Antibody Labelling Kit *BSA-Compatible, XFD594 Same Structure to Alexa Fluor™ 594* (2 Labellings)
Supplier: AAT BIOQUEST
XFD594 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 594 (Alexa Fluor® is the trademark of ThermoFisher).
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Amine reactive biotinylation reagents, EZ-Link™
Supplier: Thermo Fisher Scientific
The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.
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ReadiLink™ xtra Rapid XFD647 Antibody Labelling Kit *BSA-Compatible, XFD647 Same Structure to Alexa Fluor™ 647* (2 Labelings)
Supplier: AAT BIOQUEST
XFD647 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 647 (Alexa Fluor® is the trademark of ThermoFisher).
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ReadiLink™ DIG (Digoxigenin) Nick Translation dsDNA Labeling Kit
Supplier: AAT BIOQUEST
It is compatible with a wide variety of sample materials, including bacterial artificial chromosome (BAC) DNA, human genomic DNA, purified PCR products, supercoiled and linearized plasmid DNA.
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ReadiLink™ Biotin Nick Translation dsDNA Labeling Kit
Supplier: AAT BIOQUEST
It is compatible with a wide variety of sample materials, including bacterial artificial chromosome (BAC) DNA, human genomic DNA, purified PCR products, supercoiled and linearized plasmid DNA.
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Buccutite™ Rapid PE-Texas Red Tandem Antibody Labelling Kit
Supplier: AAT BIOQUEST
Production scale optimised for labelling 1 mg antibody per reaction. Buccutite™ Rapid PE-Texas Red tandem antibody labelling kits, designed for large-scale production, provide a streamlined approach for labelling antibodies with PE, APC, PerCP and iFluor® tandem dyes.
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iFluor® 700-Wheat Germ Agglutinin (WGA) Conjugate
Supplier: AAT BIOQUEST
The iFluor® 700 conjugate of WGA possesses a bright, far-red fluorescence characteristic of the iFluor® 700 dye (Ex/Em = 690/713 nm).