Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

Phosphate Buffered Saline with 0,05% TWEEN® 20, pH 7,4

Supplier: Merck

Phosphate buffered saline with 0,05% Tween® 20 or PBST contains PBS buffer with a low-concentration of Tween® detergent. It is a common wash buffer and due to the presence of detergent, it can easily remove or block excess components thus reducing foaming and non-specific binding on the membrane and microtiter plate wells.

Expand 1 Items

pH Buffer solutions, CX-1

Supplier: Thermo Fisher Scientific

Delivers robust reproducible gradients with the Thermo Scientific™ pH buffer platform that is applicable to a wide range of MAbs and ready to use with existing LC columns and systems, without the need to formulate mobile phases.

Expand 4 Items

Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)

Supplier: Ambion

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

Expand 4 Items

Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)

Supplier: MO BIO

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

Expand 1 Items

Buffer, TAE solution 10X concentrate (TRIS-acetate-EDTA buffer)

Supplier: ROCHE

Running Buffer, TAE buffer solution 10X concentrate (TRIS-acetate-EDTA buffer)

Expand 1 Items

Buffer, TAE solution 10X concentrate (TRIS-acetate-EDTA buffer)

Supplier: Ambion

Running Buffer, TAE buffer solution 10X concentrate (TRIS-acetate-EDTA buffer)

Expand 1 Items

RoboSep™ Buffer 2

Supplier: Stemcell Technologies

RoboSep™ buffer 2 is recommended for select EasySep™ cell separation protocols.

Expand 1 Items

Puregene RBC and Cell Lysis Solutions

Supplier: QIAGEN

For purification of archive-quality DNA from a wide range of sample types.

Expand 2 Items

HEPES Buffer Solution (1 M)

Supplier: Stemcell Technologies

HEPES buffer solution is a biological buffer used in cell culture media. Selection of suitable nutrient medium is dependent on type of cell, conditions of culture and degree of chemical definition required for the cell culture application.

Expand 1 Items

Fixation Buffer

Supplier: Stemcell Technologies

Ideal for fixing cells prior to permeabilisation, fixation buffer can be used with Intracellular Permeabilisation Buffer (10X) to conduct intracellular staining for flow cytometry. This fixation buffer can also be used to preserve fluorescently labeled cells prior to analysis by flow cytometry.

Expand 1 Items

ALDEFLUOR™ Assay Buffer

Supplier: Stemcell Technologies

Use ALDEFLUOR™ Assay Buffer to optimize the detection of aldehyde dehydrogenase-bright (ALDHbr) cells and maximize fluorescent signal stability during the ALDEFLUOR™ assay.

Expand 1 Items

Buffer, SSC solution 20X concentrate (saline-sodium citrate)

Supplier: ROCHE

Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)

Expand 1 Items

Ambion® Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l), Invitrogen™

Supplier: Ambion

Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/L)

Expand 3 Items

Buffer, TE solution (TRIS-EDTA buffer) pH 8,0

Supplier: Molekula

Cloning Buffer, TE buffer solution (TRIS-EDTA buffer) pH 8,0

Expand 1 Items

Hanks' Balanced Salt Solution, Modified without Ca++ and Mg++

Supplier: Stemcell Technologies

Hanks’ Balanced Salt Solution (HBSS) can be used for diluting, washing, irrigating or as a transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media, and provides cells with a source of water and essential inorganic ions and a carbohydrate as an energy source. Selection of suitable nutrient medium is dependent on type of cell, culture conditions, and degree of chemical definition required for the cell culture application.