Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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G-Biosciences TAE Running Buffer (50X)

Supplier: G-Biosciences

The primary use of TAE Running Buffer is in agarose electrophoresis for the separation of nucleic acids (DNA and RNA).

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Proteometer-UFT starter kit

Supplier: NOVILYTIC

The Proteometer-UFT Kit transforms any LC into an analytical system capable of identifying and quantifying titer content in crude culture filtrate in <90 seconds, without sample preparation.

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Buffers, TE solutions 10X concentrates (TRIS-EDTA buffer)

Supplier: Rockland Immunochemicals

TE buffer is a commonly used buffer solution in molecular biology, especially in procedures involving DNA, cDNA or RNA. "TE" is derived from its components: Tris, a common pH buffer, and EDTA, a molecule that chelates cations like Mg2+. The purpose of TE buffer is to solubilize DNA or RNA, while protecting it from degradation.

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Fixation Buffer (Flow Cytometry), Abcam

Supplier: Abcam

Fixation Buffer (ab314680) is a ready-to-use formaldehyde-based fixation buffer for immunofluorescence cell staining for microscopy or flow cytometry.

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HEPES Buffer pH 8.0 (1 M), Sterile

Supplier: Apollo Scientific

Aqueous solution

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In-situ hybridisation buffer (1.25X concentrate), Enzo Life Sciences

Supplier: Enzo Life Sciences

In Situ Hybridization Buffer provides a high quality hybridization medium for in situ hybridization analyses using either DNA or oligonucleotide probes labeled with biotin-, digoxigenin- or fluorescein-modified nucleotides.

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Buffer solution pH 7, Phosphate buffer

Supplier: Thermo Fisher Scientific

Potassium dihydrogen phosphate and di-Sodium hydrogen phosphate in aqueous solution, Phosphate, pH value: 7,00, ±0,02

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Proteasome assay buffer

Supplier: Enzo Life Sciences

Assay buffer from the 20S Proteasome Assay Kit for Drug Discovery.

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Tween-Tris-buffered saline (TTBS), 10X concentrate, pH 7,5

Supplier: Rockland Immunochemicals

Tween Tris buffered saline is suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Also may be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA.

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6X GelRed prestain loading buffer with tracking dye

Supplier: Biotium

6X GelRed prestain loading buffers are gel loading buffers containing density agents, tracking dyes, and GelRed dye. GelRed is a sensitive, stable and environmentally safe fluorescent nucleic acid dyes designed to replace the highly toxic ethidium bromide (EtBr).

Sustainable Options Available

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Permeabilisation buffer

Supplier: Biotium

Ready-to-use permeabilisation buffer for intracellular immunofluorescence staining for microscopy or flow cytometry.

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Buffer solution pH 10, carbonate buffer

Supplier: Thermo Fisher Scientific

CAS No.: 1332-57-6

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20x TBS-T with Tween 20, Abcam

Supplier: Abcam

Tris Buffered Saline (TBS) wash buffer with Tween 20 for use during immunolabeling techniques such as IHC.

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Fixation buffer for microscopy and flow cytometry

Supplier: Biotium

Ready-to-use formaldehyde-based fixation buffer for immunofluorescence staining for microscopy or flow cytometry.

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25x PBS Buffer pH 7.6, Abcam

Supplier: Abcam

In order to provide this product at 25X, the salts in solution are at a very high concentration.

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10X Wash Buffer PT

Supplier: Abcam

10X Wash Buffer PT (ab206977) is part of the reagents, controls and accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.