An assay is an investigative procedure in laboratory medicine, pharmacology, environmental biology, and molecular biology for qualitatively assessing or quantitatively measuring the presence or functional activity of the analyte, or the entity being targeted by the assay. Generally, assays involve biological materials or phenomena which tend to be intrinsically more complex in composition or in behavior than what can be handled by traditional chemical analysis and titration.
Fluorometric polymerase activity assay kit, EvaEZ™
Supplier: Biotium
EvaEZ™ Fluorometric Polymerase Activity Assay Kit provides an easy and accurate way to determine the activity of a nucleic acid polymerase without using radioisotopes.
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p70 S6K activity kit
Supplier: ENZO LIFE SCIENCES
The p70 S6K activity kit is an efficient assay that utilizes little sample volume. This kit gives you the ability to an end-point or kinetic assay read-out in a convenient 96-well plate based assay and offers easy sample handling protocols. The assay also offers a high signal to background ratio.
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Glucose Uptake Cell-Based Assay Kit
Supplier: Cayman Chemical
A cell-based assay for studying modulators of cellular glucose uptake.
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Cell viability assay kits, EarlyTox™ live/dead assay kits
Supplier: Molecular Devices
EarlyTox™ Cell Viability Assay Kits are a family of fluorescence-based reagents for the assessment of cell viability, cell proliferation, and various apoptosis events using mammalian cells. Optimised for use with microplate readers, these assay kits employ a no-wash, homogeneous assay protocol that enables characterisation of a full concentration-response profile of test compounds.
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ALDetect™ Lipid Peroxidation Assay kit
Supplier: ENZO LIFE SCIENCES
The assay is designed to measure either MDA alone (in hydrochloric acid) or MDA in combination with 4-hydroxyalkenals (in methanesulfonic acid). It is based on the reaction of a chromogenic reagent, N-methyl-2-phenylindole with MDA and 4-hydroxyalkenals to yield a stable chromophore with maximal absorbance at 586 nm.
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Phosphatase assay kit, BIOMOL® Green
Supplier: ENZO LIFE SCIENCES
BIOMOL® Green provides a simple and convenient method for colorimetric phosphate quantitation. It is suitable for applications include assays for phospholipid phosphatases, tyrosyl-tRNA synthetase (coupled with pyrophosphatase) and a viral RNA triphosphatase. It is most widely applied to protein phosphatase assays.
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FLUOR DE LYS® HDAC fluorometric activity assay kit
Supplier: ENZO LIFE SCIENCES
The FLUOR DE LYS® HDAC fluorometric activity assay kit is a sensitive and convenient alternative to protocols utilizing radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases. It is based on the unique FLUOR DE LYS® (Fluorimetric Histone deAcetylaseLysyl) substrate and developer combination and provides an assay that can be carried out in two simple mixing steps, all on the same 96-well plate. Histone deacetylase inhibitors have shown promise as anti-tumor agents and naturally this has stimulated interest in the screening of compounds for HDAC inhibition.
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Apoptosis assay kit (Dual), NucView™ 488
Supplier: Biotium
NucView® 488 and CF™dye (594, 640R and Texas RED) conjugated annexin V provides two apoptosis events, caspase-3 activity and phosphatidylserine (PS) translocation.
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Micro Bicinchoninic Acid (BCA) protein assay
Supplier: G-Biosciences
The Micro Bicinchoninic Acid Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilised protein solutions. This modification of the Bicinchoninic Acid (BCA) Protein Assay is suitable for dilute protein samples (0,5 to 20 µg/ml).
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Fluorescent protease assay, Fluoro™
Supplier: G-Biosciences
The Fluoro™ Protease Assay Kit is designed for the quantitative determination of proteases present in a protein sample. The assay uses fluorescein isothiocyanate (FITC)-labelled casein as a general protease substrate. The fluorescein label on the FITC-casein is highly quenched. When the proteases present in the sample of interest digest the FITC-casein substrate into smaller peptides, the quenching of the fluorescence label is relieved and the fluorescence of the substrate is increased. The fluorescence of the FITC-labeled peptide is measured with excitation at 485 nm and emission at 530 nm to determine protease activity. The kit detects picogram level of proteases present in the sample.
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Proteasome 20S Assay Kit
Supplier: ENZO LIFE SCIENCES
A QuantiZyme™ Assay System. Fluorogenic, non-radioactive assay designed to measure chymotrypsin-like protease activity of purified 20S proteasome. This 96-well assay is useful for screening inhibitors and modulators of the 20S proteasome.
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Cell viability assay kits, EarlyTox™ Caspase-3/7 and Caspase-3/7-D NucView™ 488 Assay Kits
Supplier: Molecular Devices
EarlyTox™ Cell Viability Assay Kits are a family of fluorescence-based reagents for the assessment of cell viability, cell proliferation, and various apoptosis events using mammalian cells. Optimised for use with microplate readers, these assay kits employ a no-wash, homogeneous assay protocol that enables characterisation of a full concentration-response profile of test compounds.
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Cyclic-GMP fluorescent assay kits, CatchPoint™
Supplier: Molecular Devices
CatchPoint™ cGMP Fluorescent Assay Kits measure cGMP levels via a competitive immunoassay format. The second messenger cyclic GMP (cGMP) modulates cellular processes, including smooth muscle relaxation, kidney function, and inflammation, by activating effectors such as protein kinases, cGMP-dependent phosphodiesterases, and cGMP-gated ion channels.
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Fluorometric drug discovery assay kit, SIRT5, Fluor-de-Lys®
Supplier: ENZO LIFE SCIENCES
The SIRT5 Fluorometric Drug Discovery Kit is a sensitive fluorometric assay is designed to measure the lysyl desuccinylase activity of the recombinant human SIRT5.
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ProteoStat™ PDI assay kit
Supplier: ENZO LIFE SCIENCES
The PROTEOSTAT® PDI assay kit provides a simple, homogenous assay for screening modulators of PDI enzymatic activity. This is accomplished by monitoring PDI-catalyzed reduction of insulin in the presence of Dithiothreitol (DTT), resulting in the formation of insulin aggregates which then bind avidly to the red-emitting fluorogenic PROTEOSTAT® PDI detection dye.
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FLUOR DE LYS® HDAC fluorometric cellular activity assay kit
Supplier: ENZO LIFE SCIENCES
The HDAC fluorometric cellular activity assay allows the determination of deacetylase activity within an undisturbed cellular environment and provides accurate activity information that is reflective of endogenous regulation. It also allows the study of the effects of upstream regulators on deacetylase activity and the detection of inhibitors or activators that act indirectly to affect deacetylase activity.
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Compatible Lowry protein assay
Supplier: G-Biosciences
These CL (Compatible Lowry) Protein Assay is based on the widely cited protein assay by Lowry et. al. (1951). The CL Protein Assay improves upon the traditional Lowry method to be compatible with common laboratory agents known to interfere with Lowry protein assays such as reducing agents (dithiothreitol (DTT), ß-mercaptoethanol and TCEP), detergents, chelating agents, amines, sugars, strong chaotropic buffers, salts, drugs, antibiotics, cobalt and other common laboratory agents.
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Cellular senescence activity assay
Supplier: ENZO LIFE SCIENCES
Highly reproducible SA-β-gal activity kit
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Glutaminase (GLS1) Inhibitor Screening Kit (Fluorometric)
Supplier: Abcam
Sensitive, reproducible assay for initial screening of potential glutaminase inhibitors.
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Creatinine (serum) Colorimetric Assay Kit
Supplier: Cayman Chemical
For the measurement of creatinine levels in plasma and serum.
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Millicell® µ-Migration Assay Kit
Supplier: Merck
This Kit overcomes the limitations of traditional multiwell migration assays. Its microfluidic, low-volume technology promotes a stable, diffusion-generated concentration gradient that is consistently linear and lasts for more than 48 hours.
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Fluor-de-Lys® SIRT1 fluorometric drug discovery assay kit
Supplier: ENZO LIFE SCIENCES
A Fluor-de-Lys® fluorescent assay system. The SIRT1 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human SIRT1 included in the kit.
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IHC tissue primer
Supplier: ENZO LIFE SCIENCES
Immunohistochemistry tissue primer is an enzyme blocking reagent.
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LYSO-ID® red cytotoxicity kit (GFP-CERTIFIED®) for microplates
Supplier: ENZO LIFE SCIENCES
A Rapid, Quantitative and HTS‐compatible Live Cell Cytotoxicity Assay
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Apoptosis and necrosis assay kit, NucView™488 RedDot™2
Supplier: Biotium
This NucView™488 and RedDot™2 Apoptosis and Necrosis Assay Kit contains NucView™488 caspase-3 substrate for detection of caspase-3/7 activity. The far-red dead-cell stain RedDot™2 is included for staining necrotic and late apoptotic cells that have compromised plasma membrane integrity. This kit provides a convenient tool for profiling apoptotic and necrotic cell populations by fluorescence microscopy or flow cytometry.
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DNA quantitation, SpectraMax® Quant™ dsDNA assay kits
Supplier: Molecular Devices
SpectraMax® Quant™ dsDNA Assay kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations.
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Cell viability assay kits, EarlyTox™ Caspase-3/7 R110 assay kit
Supplier: Molecular Devices
EarlyTox™ Cell Viability Assay Kits are a family of fluorescence-based reagents for the assessment of cell viability, cell proliferation, and various apoptosis events using mammalian cells. Optimised for use with microplate readers, these assay kits employ a no-wash, homogeneous assay protocol that enables characterisation of a full concentration-response profile of test compounds.
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Steady-Luc™ Firefly HTS assay kit (lyophilised)
Supplier: Biotium
Firefly luciferase is widely used as a transcriptional reporter gene for research and drug screening. The Steady-Luc™ HTS kit can be used with luminescence plate readers without sample injection systems or for high-throughput screening.