Size Exclusion Chromatography for Biomolecules - from HPLC to UHPLC
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Helping you transfer SEC methods from HPLC to UHPLC
Aqueous size exclusion chromatography (SEC) is the method of choice for the analysis of protein fragments, monomers, and aggregates under non-denaturing conditions. The use of UHPLC has triggered a remarkable increase in efficiency and throughput of LC and LC-MS methods not only for small molecules but also for biomolecules. Especially non-adsorptive chromatographic modes such as SEC benefit from the low dead volume typical for UHPLC instrumentation. The move from HPLC to UHPLC will be shown for some examples of typical SEC, SEC-MALS, and SEC-MS applications used in characterisation and quality control of biologics.
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Presented by
Patrick Endres
Patrick Endres holds a M.Sc. degree in Bio Engineering from the Karlsruhe Institute of Technology. He joined Tosoh Bioscience in 2016 and is currently responsible for the application development and benchmarking of Tosoh Bioscience’s process resins, HPLC and UHPLC columns, as well as detectors. He authored multiple application notes and scientific papers plus presents his work to scientists and customers at national and international conferences.