"protein purification system"

Supplier: Thermo Fisher Scientific

Protein A/G magnetic beads, ChIP grade, 5 ml

Supplier: Abcam

APC conjugation / labeling in < 4 hrs with 30 secs hands-on time using APC Conjugation Kit ab201807. 100% antibody recovery. - Cited in over 50 publications - APC labeled antibody is ready for use in applications like WB, ELISA, and IHC, with no need for further purification - Compatible with most standard antibody formulations - Scalable;use the same conjugation method from 10µg-100mg of antibody - Use Lightning-Link® conjugation with over 45 labels, including Alexa Fluor®, DyLight®, tandem dyes, enzymes, oligonucleotides, and gold nanoparticles

Supplier: Thermo Fisher Scientific

The Melon™ Gel Spin Plate Kit for IgG Screening offers a high throughput format for quick purification and screening of antibodies.

Supplier: G-Biosciences

Streptavidin Resin is designed for the single step small and large scale affinity purification of proteins and antibodies with a biotin tag. Biotin, a 244 Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for avidin (Ka=10¹⁵ M⁻¹) and streptavidin (Ka=10¹⁵ M⁻¹). Biotin and (strept)avidin interaction is rapid and once the bond is established it can survive up to 3M guanidinehydrochloride and extremes of pH. Biotin-avidin bonds can only be reversed by denaturing the avidin protein molecule with 8M guanidine-hydrochloride at pH 1,5 or by autoclaving. The biotinylated molecules are efficiently probed with avidin or streptavidin conjugated to reporter molecules, such as peroxidases or phosphatases.

Supplier: Cytiva

Capto™ Blue is an affinity medium for the capture of albumin, purification of enzymes, and recombinant proteins at laboratory and process scales.

Supplier: Thermo Fisher Scientific

Pierce™ protein A/G plus agarose is a high capacity agarose resin for use in a variety of antibody affinity purification methods.

Supplier: Thermo Fisher Scientific

Pierce™ Protein L agarose is a premium quality Protein L beaded agarose resin for use in a variety of antibody affinity purification methods.

Supplier: Thermo Fisher Scientific

Pierce™ Protein G agarose is a premium quality affinity resin for antibody purification.

Supplier: Cytiva

Glutathione Sepharose High Performance is used for the purification of GST-tagged proteins from bacterial lysates and other S-transferases or glutathione-dependent proteins.

Supplier: Thermo Fisher Scientific

Pierce™ chicken IgY purification kit purifies chicken antibodies from hen egg yolks using a unique delipidation reagent to separate protein from lipid and a precipitation reagent to selectively isolate the IgY fraction.

Supplier: Thermo Fisher Scientific

Pierce™ Protein A Plus agarose is a high capacity Protein A beaded agarose resin for use in a variety of antibody affinity purification methods.

Supplier: Abcam

Biotin conjugation / labeling in < 20 mins with 30 secs hands-on time using Biotin Conjugation Kit ab201795. 100% antibody recovery - Cited in over 30 publications - 15-minute incubation time - Biotin labeled antibody is ready for use in applications like WB, ELISA, and IHC, with no need for further purification - Compatible with most standard antibody formulations - Scalable;use the same conjugation method from 10µg-100mg of antibody - Use Lightning-Link® conjugation with over 45 labels, including Alexa Fluor®, DyLight®, tandem dyes, enzymes, oligonucleotides, and gold nanoparticles

Supplier: Thermo Fisher Scientific

Pierce™ Avidin agarose is purified avidin immobilised on crosslinked 6% beaded agarose for the affinity purification of biotinylated macromolecules.

Supplier: Thermo Fisher Scientific

The Protein A IgG Purification Kit is useful for routine purification of antibodies. Samples containing IgG are incubated with immobilised Protein A in a buffer that facilitates binding. After non IgG components are washed from the column, the bound IgG is recovered by elution. The supplied buffers provide for maximum immunoglobulin binding and elution efficiency with the Protein A column.

Supplier: Cytiva

Capto™ Phenyl (high sub) is used in the capture and intermediate stages of protein purification.

Supplier: Cytiva

lgG Sepharose™ 6 Fast Flow, from Cytiva, is based on the rigid Sepharose 6 Fast Flow matrix, with human IgG covalently coupled to it.