"protein purification system"
Hydrophobic interaction chromatography columns, HiTrap™ Octyl FF Sepharose™
Supplier: Cytiva
HiTrap™ Octyl FF are prepacked Octyl Sepharose™ 4 Fast Flow columns for hydrophobic interaction chromatography (HIC).
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ReadiLink™ Rapid iFluor® Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)
Supplier: AAT Bioquest
Although FITC is still the most popular fluorescent labelling dye for preparing green fluorescent bioconjugates, there are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence.
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Sheets for ÄKTA Systems, Benchkote®, Whatman™
Supplier: Whatman products (Cytiva)
These Benchkote® sheets for ÄKTA systems will protect the top buffer trays from buffer spillages and salt deposits.
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Biotin kits, HOOK™
Supplier: G-Biosciences
HOOK™ Biotin Reagent that conjugates to proteins through amines, sulfhydryls, carboxyls or carbohydrates. The amine and sulfhydryl coupling HOOK™ Biotin Reagents couple directly to the protein through their reactive groups, however the carboxyl coupling HOOK™ Biotin Reagents require a carbodiimide crosslinker and the carbohydrate coupling HOOK™ Biotin Reagents require carbohydrate oxidation before coupling.
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Reversed phase FPLC columns, RESOURCE™ RPC
Supplier: Cytiva
Resource RPC are prepacked SOURCE 15RPC columns designed for rapid screening experiments, method development, and small-scale protein purification using reversed phase chromatography.
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Affinity chromatography cartridges, cobalt, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ HisPur™ cobalt chromatography cartridges are convenient, ready-to-use pre-packed devices for efficient purification of polyhistidine-tagged proteins from bacterial, mammalian and baculovirus-infected cells. His-tagged proteins are purified from total soluble protein extract using a cobalt-charged tetradentate chelator immobilised onto 6% crosslinked agarose.
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Anti-IgG Goat Polyclonal Antibody *Cross Adsorbed* (PerCP (Peridinin-Chlorophyll Protein Complex)-Cy5.5®)
Supplier: AAT Bioquest
Anti-human secondary antibodies have well-characterised specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target.
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Anti-IgG Goat Polyclonal Antibody (PerCP (Peridinin-Chlorophyll Protein Complex)-Cy5.5®)
Supplier: AAT Bioquest
Anti-human secondary antibodies have well-characterised specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target.
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CFXpress™ E.coli Cell-free Protein Synthesis Kit
Supplier: Genscript
The GenScript CFXpress™ Cell-free E. coli Protein Synthesis Kit (CFPS) is a ready-to-use platform for rapid, high-yield in vitro protein expression from DNA or mRNA templates under the control of a T7 promoter.
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Anti-IgG Goat Polyclonal Antibody *Cross Adsorbed* (PerCP (Peridinin-Chlorophyll Protein Complex))
Supplier: AAT Bioquest
Anti-human secondary antibodies have well-characterised specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target.
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Ion exchange chromatography columns, Capto™ HiRes
Supplier: Cytiva
Capto™ HiRes Q and Capto™ HiRes S are prepacked columns for high-resolution purification of proteins and other biomolecules by ion exchange chromatography. They are well-suited for the preparation of highly pure proteins for structural studies and for protein analysis.
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Azide labeled sugars, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ azide-labeled sugars provide a highly specific approach for studying glycoproteins through in vivo metabolic labeling and chemoselective ligation.
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Reversed phase FPLC columns, SOURCE™ 15RPC ST
Supplier: Cytiva
Source 15RPC ST 4.6/100 is a prepacked stainless steel column which provides fast, high-capacity preparative protein purification with reversed phase chromatography.
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Microcystin-LR
Supplier: Enzo Life Sciences
Inhibitor of PP1 and PP2A
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Protein A HP MultiTrap™, 96-well
Supplier: Cytiva
Protein A Sepharose High Performance has high affinity for the Fc region of IgG from a variety of species.
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Anti-GST Antibody (HRP (Horseradish Peroxidase))
Supplier: Cytiva
GST is a commonly used expression marker in cloning that is also used for purification and detection of fusion proteins. This highly specific antibody to GST is conjugated to horseradish peroxidase and optimised for use in Western blotting with ECL™ Detection Reagents. The conjugate recognises multiple epitopes of GST and therefore is not reliant on functional GST for fusion protein detection. It has also been cross-absorbed to E. coli proteins so that GST-fusion proteins can be easily and specifically identified against an E. coli background.
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