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buffers

Puffer

Pufferlösungen sind pH-beständig und schützen Proben bei Zugabe von Säuren oder Laugen. Sie sind als Pulver und Flüssigkeiten erhältlich und ermöglichen die effiziente Verfolgung von Proteinen während der elektrophoretischen Isolierung und Trennung. Salzhaltige Pufferlösungen eignen sich zudem zur Kalibrierung und Standardisierung von pH-Metern für präzise Messwerte. Blockierende Substanzen verhindern die Bindung von Antikörpern an freie Membranstellen, wodurch ein störender Hintergrund entstehen würde. Die Pufferlösungen sind in verschiedenen Konzentrationen, sowohl flüssig als auch dehydriert, erhältlich und decken somit jeden Bedarf ab.

 
 
10% SDS (Sodium Dodecyl Sulfate), Corning®

10% SDS (Sodium Dodecyl Sulfate), Corning®

Supplier: Corning

Sodium Dodecyl Sulfate (SDS) is an anionic detergent with applications in SDS-PAGE protein analysis and nucleic acid purification. It is a wetting agent that is effective in both acid and alkaline solutions, and is most often used as a protein and lipid solubilization reagent. SDS is also a powerful protein denaturant. This 10% solution is prepared in 18 megaohm water and is 0.2µm filtered.

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EDTA, 0.5M pH 8.0, Corning®

EDTA, 0.5M pH 8.0, Corning®

Supplier: Corning

EDTA (ethylenediaminetetraacetic acid) is a polyamino carboxylic acid. It is a colorless, water-soluble solid and is useful as a chelating agent due to its ability to "sequester" metal ions such as Ca2+ and Fe3+.  These cations are responsible for various undesirable effects in molecular biological and cell culture processes. After being bound by EDTA, these metal ions remain in the solution but exhibit diminished reactivity.  The use of EDTA therefore allows for greater control over many experiments.

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pH buffer solutions

Supplier: Mettler - Toledo

pH Buffer solution, colour coded, pH-Wert: 9,21, Referenztemperatur: 25 °C, Blau, Plastikflasche

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TBE-Puffer-Lösung 5fach konzentriert (TRIS-Borat-EDTA-Puffer)

Supplier: PanReac AppliChem

Laufpuffer, TBE-Puffer-Lösung 5fach konzentriert (TRIS-Borat-EDTA-Puffer)

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pH-Pufferlösungen

pH-Pufferlösungen

Supplier: PanReac AppliChem

pH-Pufferlösung, pH-Wert: 7,00, Referenztemperatur: 20 °C

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pH buffer solutions, certified (DKD)

Supplier: Mettler - Toledo

DKD certified buffer solution, pH-Wert: 7,00, 6× 250 ml

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Y-PER™ plus Yeast Protein Extraction Reagent, Dialysable

Y-PER™ plus Yeast Protein Extraction Reagent, Dialysable

Supplier: Thermo Fisher Scientific

This extraction reagent is formulated to effectively lyse yeast and bacterial cells to extract and solubilise proteins for analysis.

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TRIS-Glycin-SDS-Puffer-Lösung 10fach konzentriert (TGS-Puffer) pH 8,3

Supplier: PanReac AppliChem

Laufpuffer, TRIS-Glycin-SDS-Puffer-Lösung 10fach konzentriert (TGS-Puffer) pH 8,3

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Saline solutions

Supplier: BALIS

Cell culture buffer, Physiological saline solution, 9 ml, no standards

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TRIS-Glycin-Puffer-Lösung 10fach konzentriert (TG-Puffer), Sigma-Aldrich®

Supplier: Merck

Elektrophorese-Puffer, Transfer buffer, TRIS-Glycin-Puffer-Lösung 10fach konzentriert (TG-Puffer)

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Mini flip-top vial with 9 ml maximum recovery diluent, BPPFV9MRD

Mini flip-top vial with 9 ml maximum recovery diluent, BPPFV9MRD

Supplier: Neogen

The 3M™ Mini Flip-Top Vial with 9 ml Maximum Recovery Diluent is a leak-resistant plastic cylinder with a flip-open lid that seals securely for safe shipping and handling.

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Subcellular Protein Fractionation Kit for Cultured Cells

Subcellular Protein Fractionation Kit for Cultured Cells

Supplier: Thermo Fisher Scientific

This kit is ideal for segregation and enrichment of proteins from five different cellular compartments.

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TE-Puffer-Lösung 100fach konzentriert (TRIS-EDTA-Puffer), Sigma-Aldrich®

Supplier: Merck

Cloning Buffer, TE-Puffer-Lösung 100fach konzentriert (TRIS-EDTA-Puffer)

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Proteometer-UFT kit

Proteometer-UFT kit

Supplier: NOVILYTIC

The Proteometer-UFT Kit transforms any LC into an analytical system capable of identifying and quantifying titer content in crude culture filtrate in <90 seconds, without sample preparation.

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Proteometer-L-Verbrauchsmaterialien-Kit

Proteometer-L-Verbrauchsmaterialien-Kit

Supplier: NOVILYTIC

The Proteometer-L Kit transforms any LC into an analytical system capable of identifying and quantifying titer and relative aggregate content in crude culture filtrate in <10 minutes, without sample preparation.

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Nuclear Extraction Kit, Abcam

Supplier: Abcam

Nuclear extraction kit provides a simple and selective method for nuclear protein extraction and fractionation from cells or tissue samples in just 1 hour. This kit includes all necessary reagents to obtain high yields of nuclear proteins suitable for various downstream applications. - Cited in over 255 publications - High retention of enzyme activity – contains 80% less salt than conventional kits and no SDS, allowing better enzyme activity retention - Suitable for western blotting, protein-DNA binding assays, nuclear enzyme assays, and more - Fast, simple procedure that ensures optimized nuclear protein isolation

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