buffers
Pufferlösungen sind pH-beständig und schützen Proben bei Zugabe von Säuren oder Laugen. Sie sind als Pulver und Flüssigkeiten erhältlich und ermöglichen die effiziente Verfolgung von Proteinen während der elektrophoretischen Isolierung und Trennung. Salzhaltige Pufferlösungen eignen sich zudem zur Kalibrierung und Standardisierung von pH-Metern für präzise Messwerte. Blockierende Substanzen verhindern die Bindung von Antikörpern an freie Membranstellen, wodurch ein störender Hintergrund entstehen würde. Die Pufferlösungen sind in verschiedenen Konzentrationen, sowohl flüssig als auch dehydriert, erhältlich und decken somit jeden Bedarf ab.
10% SDS (Sodium Dodecyl Sulfate), Corning®
Supplier: Corning
Sodium Dodecyl Sulfate (SDS) is an anionic detergent with applications in SDS-PAGE protein analysis and nucleic acid purification. It is a wetting agent that is effective in both acid and alkaline solutions, and is most often used as a protein and lipid solubilization reagent. SDS is also a powerful protein denaturant. This 10% solution is prepared in 18 megaohm water and is 0.2µm filtered.
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EDTA, 0.5M pH 8.0, Corning®
Supplier: Corning
EDTA (ethylenediaminetetraacetic acid) is a polyamino carboxylic acid. It is a colorless, water-soluble solid and is useful as a chelating agent due to its ability to "sequester" metal ions such as Ca2+ and Fe3+. These cations are responsible for various undesirable effects in molecular biological and cell culture processes. After being bound by EDTA, these metal ions remain in the solution but exhibit diminished reactivity. The use of EDTA therefore allows for greater control over many experiments.
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pH buffer solutions
Supplier: Mettler - Toledo
pH Buffer solution, colour coded, pH-Wert: 9,21, Referenztemperatur: 25 °C, Blau, Plastikflasche
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TBE-Puffer-Lösung 5fach konzentriert (TRIS-Borat-EDTA-Puffer)
Supplier: PanReac AppliChem
Laufpuffer, TBE-Puffer-Lösung 5fach konzentriert (TRIS-Borat-EDTA-Puffer)
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pH-Pufferlösungen
Supplier: PanReac AppliChem
pH-Pufferlösung, pH-Wert: 7,00, Referenztemperatur: 20 °C
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pH buffer solutions, certified (DKD)
Supplier: Mettler - Toledo
DKD certified buffer solution, pH-Wert: 7,00, 6× 250 ml
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Y-PER™ plus Yeast Protein Extraction Reagent, Dialysable
Supplier: Thermo Fisher Scientific
This extraction reagent is formulated to effectively lyse yeast and bacterial cells to extract and solubilise proteins for analysis.
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TRIS-Glycin-SDS-Puffer-Lösung 10fach konzentriert (TGS-Puffer) pH 8,3
Supplier: PanReac AppliChem
Laufpuffer, TRIS-Glycin-SDS-Puffer-Lösung 10fach konzentriert (TGS-Puffer) pH 8,3
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Saline solutions
Supplier: BALIS
Cell culture buffer, Physiological saline solution, 9 ml, no standards
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TRIS-Glycin-Puffer-Lösung 10fach konzentriert (TG-Puffer), Sigma-Aldrich®
Supplier: Merck
Elektrophorese-Puffer, Transfer buffer, TRIS-Glycin-Puffer-Lösung 10fach konzentriert (TG-Puffer)
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Mini flip-top vial with 9 ml maximum recovery diluent, BPPFV9MRD
Supplier: Neogen
The 3M™ Mini Flip-Top Vial with 9 ml Maximum Recovery Diluent is a leak-resistant plastic cylinder with a flip-open lid that seals securely for safe shipping and handling.
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Subcellular Protein Fractionation Kit for Cultured Cells
Supplier: Thermo Fisher Scientific
This kit is ideal for segregation and enrichment of proteins from five different cellular compartments.
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TE-Puffer-Lösung 100fach konzentriert (TRIS-EDTA-Puffer), Sigma-Aldrich®
Supplier: Merck
Cloning Buffer, TE-Puffer-Lösung 100fach konzentriert (TRIS-EDTA-Puffer)
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Proteometer-UFT kit
Supplier: NOVILYTIC
The Proteometer-UFT Kit transforms any LC into an analytical system capable of identifying and quantifying titer content in crude culture filtrate in <90 seconds, without sample preparation.
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Proteometer-L-Verbrauchsmaterialien-Kit
Supplier: NOVILYTIC
The Proteometer-L Kit transforms any LC into an analytical system capable of identifying and quantifying titer and relative aggregate content in crude culture filtrate in <10 minutes, without sample preparation.
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Nuclear Extraction Kit, Abcam
Supplier: Abcam
Nuclear extraction kit provides a simple and selective method for nuclear protein extraction and fractionation from cells or tissue samples in just 1 hour. This kit includes all necessary reagents to obtain high yields of nuclear proteins suitable for various downstream applications. - Cited in over 255 publications - High retention of enzyme activity – contains 80% less salt than conventional kits and no SDS, allowing better enzyme activity retention - Suitable for western blotting, protein-DNA binding assays, nuclear enzyme assays, and more - Fast, simple procedure that ensures optimized nuclear protein isolation



