buffers

Pufferlösungen sind pH-beständig und schützen Proben bei Zugabe von Säuren oder Laugen. Sie sind als Pulver und Flüssigkeiten erhältlich und ermöglichen die effiziente Verfolgung von Proteinen während der elektrophoretischen Isolierung und Trennung. Salzhaltige Pufferlösungen eignen sich zudem zur Kalibrierung und Standardisierung von pH-Metern für präzise Messwerte. Blockierende Substanzen verhindern die Bindung von Antikörpern an freie Membranstellen, wodurch ein störender Hintergrund entstehen würde. Die Pufferlösungen sind in verschiedenen Konzentrationen, sowohl flüssig als auch dehydriert, erhältlich und decken somit jeden Bedarf ab.

Competent cell buffer

Supplier: Rockland Immunochemicals

This product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of 60 mM Calcium Chloride, 15% v/v Glycerol, 10mM Tris HCl at a pH of 7,5.

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RBC Lysis Buffer (10X)

Supplier: Tonbo Biosciences

RBC Lysis Buffer (10X) is a concentrated ammonium chloride-based lysing reagent.

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Permeabilization Buffer (Flow Cytometry), Abcam

Supplier: Abcam

Permeabilization Buffer (ab314681) is a ready to use permeabilization buffer for intracellular immunofluorescence staining for microscopy or flow cytometry.

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Research buffers and reagents

Supplier: G-Biosciences

These DNase- and RNase-free buffers and reagents are designed for high quality research.

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Fixation Buffer (Flow Cytometry), Abcam

Supplier: Abcam

Fixation Buffer (ab314680) is a ready-to-use formaldehyde-based fixation buffer for immunofluorescence cell staining for microscopy or flow cytometry.

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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L)

Supplier: Thermo Fisher Scientific

Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)

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North2South™ Hybridisation Buffer

Supplier: Thermo Fisher Scientific

North2South™ hybridisation buffer is an aqueous buffer which is used with the North2South™ chemiluminescent detection kit.

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VWR®, Buffers, Sodium acetate

Supplier: VWR Chemicals

Sodium acetate buffer B, VWR®

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G-Biosciences Sodium Chloride-Sodium Phosphate-EDTA Buffers (20X)

Supplier: G-Biosciences

Saline-sodium phosphate-EDTA hybridisation buffer [20X].

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Antigen Retrieval Buffer (100X Tris Buffer, pH 10.0), Abcam

Supplier: Abcam

Antigen Retrieval Buffer (100X Tris Buffer, pH 10.0) is a special solution that enables rehydrate and target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step.

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Cell lysis buffer, RIPA

Supplier: Enzo Life Sciences

The RIPA liquid cell lysis buffer composes of 50 mM TRIS-hydrogen chloride, pH 7,4, containing 150 mM sodium chloride, 1 mM EDTA, 1 mM EGTA, 1,2% Triton X-100, 0,5% sodium deoxycholate, and 0,1% SDS.

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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L)

Supplier: Cayman Chemical

Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)

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Caspase assay buffer

Supplier: Enzo Life Sciences

50 mM HEPES, pH 7,4, 100 mM NaCl, 0,1% CHAPS, 10 mM DTT, 1 mM EDTA, 10% glycerol.

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Antigen Retrieval Buffer (100X EDTA Buffer, pH 8.0), Abcam

Supplier: Abcam

Antigen Retrieval Buffer (100X EDTA Buffer, pH 8.0) is a special solution that enables rehydrate and target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step.