buffers
Pufferlösungen sind pH-beständig und schützen Proben bei Zugabe von Säuren oder Laugen. Sie sind als Pulver und Flüssigkeiten erhältlich und ermöglichen die effiziente Verfolgung von Proteinen während der elektrophoretischen Isolierung und Trennung. Salzhaltige Pufferlösungen eignen sich zudem zur Kalibrierung und Standardisierung von pH-Metern für präzise Messwerte. Blockierende Substanzen verhindern die Bindung von Antikörpern an freie Membranstellen, wodurch ein störender Hintergrund entstehen würde. Die Pufferlösungen sind in verschiedenen Konzentrationen, sowohl flüssig als auch dehydriert, erhältlich und decken somit jeden Bedarf ab.
Blocking buffer, fish gelatin
Supplier: Biotium
Fish gelatin is commonly used in blocking solutions to minimise background noise in immunodetection procedures such as Western Blotting and immunostaining. Fish gelatin can be used to block non-specific binding sites on positively charged nylon or PVDF membranes or for blocking and dilution of antibodies during immunostaining procedures. Unlike BSA or milk, fish gelatin does not contain IgG or serum proteins that could cross-react with mammalian antibodies.
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G-Biosciences 10X Concentrated TE Buffers (Tris-EDTA Buffer)
Supplier: G-Biosciences
The tris buffering agent and EDTA metal chelating properties help protect DNA and RNA.
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PBS tablets
Supplier: PanReac AppliChem
Tablets for preparation of 100 ml phosphate buffered saline (PBS).
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TBE-Puffer-Lösung 5fach konzentriert (TRIS-Borat-EDTA-Puffer)
Supplier: Biotium
Laufpuffer, TBE-Puffer-Lösung 5fach konzentriert (TRIS-Borat-EDTA-Puffer)
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Gram Negative lysis buffer
Supplier: G-Biosciences
Gram-negative lysis buffer is an extraction buffer for soluble proteins from Gram-negative bacteria. It is a proprietary improvement on the lysozyme based lysis in combination with various salts and agents, which allows extraction of soluble proteins. This buffer has been tested for use with several widely used bacteria including E.coli strains.
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Buffer, PBS tablets
Supplier: PanReac AppliChem
PBS Tablets for preparation of 200 ml phosphate buffered saline (PBS). Composition of 140 mM sodium chloride, 2,7 mM potassium chloride and 10 mM phosphate.
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Nuclear Extraction Kit, Abcam
Supplier: Abcam
Nuclear extraction kit provides a simple and selective method for nuclear protein extraction and fractionation from cells or tissue samples in just 1 hour. This kit includes all necessary reagents to obtain high yields of nuclear proteins suitable for various downstream applications. - Cited in over 255 publications - High retention of enzyme activity – contains 80% less salt than conventional kits and no SDS, allowing better enzyme activity retention - Suitable for western blotting, protein-DNA binding assays, nuclear enzyme assays, and more - Fast, simple procedure that ensures optimized nuclear protein isolation
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Nuclear (Nucleic Acid-Free) Extraction Kit, Abcam
Supplier: Abcam
Nuclear (Nucleic Acid-Free) Extraction Kit (ab113477) provides a simple and selective method for extracting nuclear proteins (nucleic-acid free) in just 1 hour for a variety of applications such as western blotting, protein-DNA binding assays, or any other procedures requiring optimized nucleic acid-free nuclear proteins.
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TRIS Pufferlösung pH 7 (1 mol/l)
Supplier: Cayman Chemical
Cloning Buffer, TRIS Pufferlösung pH 7 (1 mol/l)
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TRIS Pufferlösung pH 7,5 (1 mol/l)
Supplier: Cayman Chemical
Cloning Buffer, TRIS Pufferlösung pH 7,5 (1 mol/l)
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TRIS Pufferlösung pH 8 (1 mol/l)
Supplier: Cayman Chemical
Cloning Buffer, TRIS Pufferlösung pH 8 (1 mol/l)
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Buffers, sodium phosphate buffer solutions (0,5 mol/L)
Supplier: Cayman Chemical
Sodium phosphate stock solution (0,5 mol/L), pH-Wert: 6,0
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Kaliumphosphatpuffer (0,5 mol / l) Lösungen
Supplier: Cayman Chemical
Kaliumphosphat Pufferlösung (0,5 mol/l), pH-Wert: 6,0
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HEPES Pufferlösung (1 mol/l)
Supplier: Cayman Chemical
HEPES Pufferlösung (1 mol/l)
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Buffer, EDTA buffer solution pH 8,0 (0,5 mol/L)
Supplier: Cayman Chemical
Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)
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TBS-Puffer-Lösung 10fach konzentriert (TRIS-gepufferte Salzösung) mit Phenolrot pH 7,4
Supplier: Cayman Chemical
250 mM Tris-base, pH 7,4, 1,37 M sodium chloride, containing 27 mM KCl, 0,4 mM phenol red.
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