buffers
Pufferlösungen sind pH-beständig und schützen Proben bei Zugabe von Säuren oder Laugen. Sie sind als Pulver und Flüssigkeiten erhältlich und ermöglichen die effiziente Verfolgung von Proteinen während der elektrophoretischen Isolierung und Trennung. Salzhaltige Pufferlösungen eignen sich zudem zur Kalibrierung und Standardisierung von pH-Metern für präzise Messwerte. Blockierende Substanzen verhindern die Bindung von Antikörpern an freie Membranstellen, wodurch ein störender Hintergrund entstehen würde. Die Pufferlösungen sind in verschiedenen Konzentrationen, sowohl flüssig als auch dehydriert, erhältlich und decken somit jeden Bedarf ab.
Blocking buffers, FirstChoice™
Supplier: G-Biosciences
FirstChoice™ Blocking Buffer is ideal as a first choice for optimization of new assays and systems, or when determining the optimal blocking buffer for elimination of non specific binding sites in ELISA, blotting, immunohistochemistry and other applications. Buffers are conveniently supplied in widely used TBS (Tris-buffered saline at pH 7,5) and PBS (phosphate-buffered saline at pH 7,5) buffers as well as in separate formulations containing Tween® 20 for improving blocking efficiencies.
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Proteasome assay buffer
Supplier: Enzo Life Sciences
Assay buffer from the 20S Proteasome Assay Kit for Drug Discovery.
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PTP1B 2x assay buffer
Supplier: Enzo Life Sciences
PTP1B 2x assay buffer
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Dry buffer packs, JAW™
Supplier: G-Biosciences
Premixed buffer powders that simply require adding water to generate a ready-to-use buffer.
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MMP-1 colorimetric assay buffer
Supplier: Enzo Life Sciences
MMP-1 colorimetric assay buffer
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TE Buffer (1X) pH 8,0 for Molecular Biology
Supplier: Apollo Scientific
Cloning Buffer, TE buffer (1X)
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Hybridisation buffers
Supplier: G-Biosciences
The hybridisation buffers like Church & Gilbert’s, Denhardt solution, hybridisation denaturing solution, hybridisation neutralising solution, SSC, SSPE, TMAC Hyb solution and TNT Buffer are used in wide range of applications. Church & Gilbert’s Hybridization Buffer is prepared from molecular biology grade SDS (sodium dodecyl sulfate), monosodium phosphate (NaHPO₄), EDTA (ethylenediaminetetracetic acid) and bovine serum albumin (BSA) Fraction V using DNase, RNase and Protease-free water. It consists of 1mM EDTA, 1% BSA, 0,5M sodium phosphate monobasic, and 7% SDS (at a pH of 7,2).
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IHC Wash Buffer, Enzo Life Sciences
Supplier: Enzo Life Sciences
The IHC wash buffer is a for washing and removing excess reagents during immunostaining.
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Wash buffer concentrate
Supplier: Enzo Life Sciences
Liquid. TRIS buffered saline containing detergents.
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Buffer concentrate, citrate phosphate, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
These buffers are used to elute adult hemoglobin by the procedure of Oski and Naiman, a modification of the Kleihauer Betke procedure for the demonstration of fetal hemoglobin.
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Wash buffer salts, lyophilised, Enzo Life Sciences
Supplier: Enzo Life Sciences
Wash Buffer Salts can be used in tissue preparations, immunohistochemical staining procedures and in assays that require HRP-linked detection reagents.
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Fixation buffer for microscopy and flow cytometry
Supplier: Biotium
Ready-to-use formaldehyde-based fixation buffer for immunofluorescence staining for microscopy or flow cytometry.
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Gram Negative lysis buffer
Supplier: G-Biosciences
Gram-negative lysis buffer is an extraction buffer for soluble proteins from Gram-negative bacteria. It is a proprietary improvement on the lysozyme based lysis in combination with various salts and agents, which allows extraction of soluble proteins. This buffer has been tested for use with several widely used bacteria including E.coli strains.
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Protein Transfer buffers
Supplier: G-Biosciences
Efficient™ Western Transfer Buffer (20X) achieves increased protein transfer efficiency. This ready-to-use transfer buffer is prepared for optimal conductivity and efficient protein transfer, without generating excessive heat or transfer distortion.
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RIPA cell lysis buffer 2
Supplier: Enzo Life Sciences
RIPA cell lysis buffer 2, Enzo Life Sciences
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