"Staining Solutions"

Supplier: SIGMA ALDRICH MICROSCOPY

Oil red O is a lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. In histology, a supersaturated solution of oil red O in isopropanol may be used to stain fat in tissue.

Supplier: SIGMA ALDRICH MICROSCOPY

Bouin's solution is an excellent fixative for preserving soft and delicate structures. It is prepared with saturated picric acid, formaldehyde and acetic acid.

Supplier: G-Biosciences

Conventional stain used for Hematoxylin and Eosin (H&E) staining in histology and cytology procedures. Ready to use formulation optimized for histology and cytology procedures where reproducible results are important.

Supplier: Abcam

Cationic dye. Forms insoluble complexes with glycosaminoglycans in solution. Stains interphase nuclei, especially mitotic figures.

Supplier: SIGMA ALDRICH MICROSCOPY

Alcian Blue solution has been used in the histological staining of acidic glycosaminoglycans (GAGs; mucopolysaccharides) that are carboxylated or sulfated. Sulfated glycosaminoglycans appear to be the preferred substrate for alcian blue at pH 2.5. Alcian blue has been used to stain hyaluronan (HA), a nonsulfated GAG, but the intensity of the staining of HA is affected by the method of tissue fixation used. Alcian blue staining of GAGs in tissue may be modified through the addition of an electrolyte such as magnesium chloride.

Supplier: SIGMA ALDRICH MICROSCOPY

Wright′s Stain solution is commercially available and is generally used in hematology. Its preparation involves heating methylene blue with sodium bicarbonate and combining the mixture with eosin. It is alkaline, hence, its concentration is continuously changing due to the progressive oxidizing action of the alkali. Therefore, Giemsa′s stain is added to preserve the azurophilic properties of polychrome dyes.

Supplier: Abcam

Abcam's Flow Cytometry Lysing Solution is a ready-to-use solution formulated for lysing erythrocytes following monoclonal antibody staining of whole blood.

Supplier: SIGMA ALDRICH MICROSCOPY

Brilliant Blue G is a methanol based stain which is especially designed for staining protein in polyacrylamide gels, but will also stain protein in agarose gels (IEF). The stain contains methanol and acetic acid so gels do not require fixing prior to staining. Water is added directly to the bottle to produce 1 liter of ready-to-use staining solution.

Supplier: G-Biosciences

The stain uses an enhanced Periodic Acid-Schiff (PAS) method for detection of glycoprotein sugars. The supplied agent first oxidises the cis-diol sugar groups to aldehydes. The aldehyde groups react with the sensitive glycoprotein stain solution forming Schiff bonds and producing strong magenta colour bands. RAPIDstain™ can be used after glycoprotein staining to detect non-glycosylated proteins and the use of the stain enhances glycoprotein staining. The glycoprotein stainis highly convenient as all the key reagents required for staining are supplied and a unique positive & negative control is included.

Supplier: Abcam

Nuclear fast red stain solution (pH 2 - 4) is a stain for histological applications. - Stains nuclei red and cytoplasm pale pink - Contains 80% water, 5% aluminium sulfate, 0.2% nuclear fast red - Enhanced stability - Prevents precipitation over time

Supplier: Biotium

Activated charcoal retains ethidium bromide, GelRed™ and GelGreen™ efficiently in a convenient bag for decontamination of used gel staining solutions. Each bag has a total capacity of at least 10 mg ethidium bromide.

Supplier: Merck

Azure II (C.I. 52010/ 52015) is a dry dye for the preparation of a color staining solution used for the staining of blood and bone marrow smears of human origin. The dye is a CE certified IVD product and has a constant dye content and a reliable high quality, which is one of the major precondition for a reproducible diagnostic staining.

Supplier: Merck

Löffler's methylene blue solution is generally used as bacteriological counter staining of, e.g. of gonococcae, lactic acid bacteria and for visualization of the pole corpuscles of pasteurella.

Supplier: Abcam

Crystal Violet Solution ab246820 is the standard dye for staining gram positive bacteria.

Supplier: Biotium

Ponceau S staining solution is used for the detection of proteins on PVDF and nitrocellulose membranes with a limit of detection of about 250 ng of transferred protein.

Supplier: RAL

Staining with Quick TB Kit allows to detect mycobacteria, also called A.F.B (Acid Alcohol Fast Bacilli) through a cold and fast staining technique. Carbolic Fuchsin RAL stains mycobacteria pink. Armand Solution stains the background of the preparation blue.