1205 Results for: "Staining Solutions"
Ethidium homodimer I Verwendung in der nuklearen Färbung
Supplier: Biotium
Ethidium Homodimer, first developed by Dr. Le Pecq and his colleagues, is a high-affinity fluorescent nucleic acid stain. It binds to both DNA and RNA in a sequence-independent manner and with a >30-fold fluorescence enhancement. The DNA binding of each Ethidium Homodimer covers four base pairs and is believed to occur by intercalation. Because the dye is highly positively charged, it cannot cross cell membranes to stain living cells. It is useful for detecting nucleic acids in solution, or for selectively staining dead cells with damaged plasma membranes.
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Formalinlösung-Lösung 10% (synthetisch), neutral gepuffert, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Formalin solution, neutral buffered, 10% is the most commonly used fixative for light microscopy. It requires a relatively short period of fixation. It is suitable for the long-term storage of tissue. Once properly fixed, the tissue should be able to withstand the subsequent stages of tissue processing or staining.
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Ethidium homodimer I 2 mM in DMSO Verwendung in der nuklearen Färbung
Supplier: Biotium
Ethidium Homodimer, first developed by Dr. Le Pecq and his colleagues, is a high-affinity fluorescent nucleic acid stain. It binds to both DNA and RNA in a sequence-independent manner and with a >30-fold fluorescence enhancement. The DNA binding of each Ethidium Homodimer covers four base pairs and is believed to occur by intercalation. Because the dye is highly positively charged, it cannot cross cell membranes to stain living cells. It is useful for detecting nucleic acids in solution, or for selectively staining dead cells with damaged plasma membranes.
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Formalinlösung 10% Fixativ für die Histologie, neutral gepuffert, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Formalin solution (10%, neutral buffered) is a widely used fixative for samples processed for light microscopy. It requires a relatively short period of fixation. It is suitable for the long-term storage of tissue. Once properly fixed, the tissue should be able to withstand the subsequent stages of tissue processing or staining.
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Hydroxystilbamidine bis(methanesulphonate) 4% in Wasser fluoreszierender Farbstoff, für die Histologie
Supplier: Biotium
Hydroxystilbamidine (also called Fluoro-Gold™) has been used extensively as a retrograde tracer for neurons and also a histochemical stain. The 4% solution in deionized H2O is a convenient, ready-to-use form..
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5-Brom-4-chlor-3-indolylphosphat p-Toluidinsalz (BCIP (p-Toluidinsalz), X-Phosphat) Substrat ≥99%
Supplier: Thermo Fisher Scientific
Thermo Scientific Pierce NBT and BCIP Substrates are powders and ready-made solutions of nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate for chromogenic blot and IHC staining with alkaline phosphatase probes.
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TE buffer, pH 7,4, RNAse free
Supplier: Thermo Fisher Scientific
TE buffer is a commonly used buffer solution in procedures involving DNA or RNA. It is used for storing and diluting DNA and RNA. Treatment with TE buffer enhances the staining intensity of antibodies in the immunohistochemical detection of certain proteins.
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Polyester Lab Microscope Slide Labels, Core Small
Supplier: Brady
This B-488 material combined with the R-4300 series ribbon provides a solution that can withstand various chemicals without damage, such as xylene and other chemicals commonly used in slide staining. Printing does not smear, the label does not curl, and there is no discoloration that could make barcodes illegible. The label adheres reliably to the slide.
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Ölrot O, Certistain® für die Mikroskopie, Sigma-Aldrich®
Supplier: Merck
The CERTISTAIN® dry dye Oil red O (C.I. 26125) is used for the preparation of a staining solution that can be used for the detection of lipids in histological cryo sections.
As CERTISTAIN® dye it is chemically quality-certified according to highest standards and strict specifications. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations.
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Histo/Zyme
Supplier: SIGMA ALDRICH MICROSCOPY
Histo/Zyme is a stabilized proteolytic enzyme in buffered solution, used to unmask immunoreactive sites altered by fixation and/or the embedding process. Treatment with a proteolytic enzyme in immunohistochemistry (IHC) is required to digest excess linkages and expose the antigen. The use of Histo/Zyme results in superior staining over traditionally used enzyme pretreatments, such as pepsin and trypsin. Moreover, Histo/Zyme requires only a brief incubation at room temperature. Performing antigen recovery with Histo/Zyme will allow you to dilute the primary antibody 5 to 10 fold further and achieve optimal staining.
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Formalinlösung-Lösung 10%, neutral gepuffert, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Formalin solution, neutral buffered, 10% is the most commonly used fixative for light microscopy. It requires a relatively short period of fixation. It is suitable for the long-term storage of tissue. Once properly fixed, the tissue should be able to withstand the subsequent stages of tissue processing or staining. 10% NBF is well suited for large throughput laboratories, and requires a relatively short period of fixation, but can also be used for the long-term storage of tissue. Formalin fixation is thought to form between the aldehydes and the proteins, creating a gel, thus retaining cellular constituents in their in vivo relationship.
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3D Clear tissue clearing reagents and buffers, Corning®
Supplier: Corning
In our Starter Kit we have combined 30 ml of Corning 3D Clear with the four buffer solutions required to process 3D tissue cultures for immunolabelling. All you need to do is add your immunolabels and nuclear stains. This kit comes with enough material to process three 96-well plates of 3D cell culture models.
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APO-BRDU™ kit, TUNEL Assay
Supplier: Tonbo Biosciences
The APO-DIRECT™ reagent kit provides a two color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labelling) assay for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry. The kit contains the instructions and reagents required for measuring apoptosis in cells including: positive and negative control cells for assessing reagent performance; washing, reaction and rinsing buffers for processing individual steps in the assay; terminal deoxynucleotidyl tranferase enzyme (TdT), fluorescein isothiocyanate (FITC) deoxyuridine triphosphate (FITC-dUTP) for labeling DNA breaks and PI/RNase A solution for counter staining the total DNA.
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Anti-BrdU Mouse Monoclonal Antibody (FITC) [clone: PRB-1]
Supplier: Tonbo Biosciences
The incorporation of BrdU into newly synthesized DNA by actively cycling cells is one method for measuring the changing amount of cellular DNA during cell proliferation through each of the cell cycle phases. As a thymidine analog, BrdU is preferentially incorporated into newly replicated DNA which can then be subsequently labeled and analyzed to determine relative DNA content and cell cycle position. Incorporation of BrdU is most commonly detected using anti-BrdU antibodies. A BrdU solution is provided for exposure of actively cycling cells to incorporate BrdU. The EZ-BrdU Kit employs an acid denaturation step. The mild acid method used helps reduce damage to other cellular proteins. After the denaturation step, cells are stained with a FITC anti-BrdU antibody and total DNA is counterstained with a PI/RNase A solution. Two color flow cytometry can then be used to analyze cells that have incorporated BrdU (proliferating cells) in terms of their cell cycle position (G0/1, S, or G2/M phase). The EZ-BrdU Kit is shipped in one container and consists of two packages. Upon arrival one should be stored at 2-8°C and the other at -20°C.
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Ethidiumbromid
Supplier: MP Biomedicals
Ethidium bromide(EtBr) has been used extensively to reduce mitochondrial DNA copy number in proliferating cells and most commonly used in nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/mL). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA.4 Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase. A 10mg/mL easy-to-use solution of ethidium bromide in specially filtered, deionized water which is excellent for nucleic acid electrophoresis and purification applications, eliminates the dust hazard associated with powdered ethidium bromide and saves time spent on weighing and mixing. Used in the fluorometric detection of double stranded nucleic acids in gel electrophoresis. Also acts as an RNA polymerase inhibitor, and in separation of high molecular weight DNA's. It confers a deep red stain to DNA by acting as an intercalating agent between the starch bases. Subsequent fluorescence techniques enable the extraction and characterization of DNA bands. Biochem/physiol Actions: Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.
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Elastin Färbelösung acc. Weigert, Sigma-Aldrich®
Supplier: Merck
Used to stain elastic fibers
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Eisenhämatoxylin Kit (Weigert's) Färbemittel für Histologie, Sigma-Aldrich®
Supplier: Merck
Nuclear staining in connective tissue staining methods
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Papanicolaous Lösung 1a, Harris' Hämatoxylinlösung Verwendung in der nuklearen Färbung, für Zytologie, Sigma-Aldrich®
Supplier: Merck
Nuclear staining in cytology
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Gram Entfärbelösung zur Gramfärbung, Sigma-Aldrich®
Supplier: Merck
Used for Gram staining
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Papanicolaous Lösung 1b Polychromlösung S Verwendung in der nuklearen Färbung, für Zytologie, Sigma-Aldrich®
Supplier: Merck
Nuclear staining in cytology
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Papanicolaous Lösung 2b Orange II Lösung Färbemittel für Zytologie, Sigma-Aldrich®
Supplier: Merck
Cytoplasm staining of mature and keratinized cells in cytology
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Acid hematoxylin solution
Supplier: SIGMA ALDRICH MICROSCOPY
Gill formulation No. 2 may be used for cytology and/or histology as a progressive or regressive stain depending on length of staining time. 4 g/L certified hematoxylin.
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Papanicolaou's Lösung 2a Orange G (OG 6), Sigma-Aldrich®
Supplier: Merck
Cytoplasm staining of mature and keratinized cells in cytology
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Papanicolaous Lösung 3c Polychromlösung EA65 Verwendung in der nuklearen Färbung, für Zytologie, Sigma-Aldrich®
Supplier: Merck
Cytoplasm staining esp. for non-gynecological specimen, color effect light red
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Picrofuchsin solution acc. to van Gieson Verwendung in der nuklearen Färbung, für die Histologie, Sigma-Aldrich®
Supplier: Merck
For staining collagenous connective tissue, muscle tissue, and cornified epithelium
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Papanicolaous Lösung 3d Polychromlösung EA65 Verwendung in der nuklearen Färbung, für Zytologie, Sigma-Aldrich®
Supplier: Merck
Cytoplasm staining esp. for non-gynecological specimens, color effect pale blue-green
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Hemacolor® Schnelles Färben von Blutausstrich-Lösung III Färbereagenz, Sigma-Aldrich®
Supplier: Merck
Color reagent blue (thiazine solution)
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Hemacolor® Schnelles Färben von Blutausstrich-Lösung II Färbereagenz, Sigma-Aldrich®
Supplier: Merck
Color reagent red (eosin solution)
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Nuclear fast red-aluminum sulphate solution 0,1% Verwendung in der nuklearen Färbung, für die Histologie, Sigma-Aldrich®
Supplier: Merck
For nuclear staining and for counterstaining in histochemical reactions e.g. Berlin blue
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Hemacolor® Schnelles Färben von Blutausstrich-Lösung I fixatives Reagens, Sigma-Aldrich®
Supplier: Merck
Fixing solution (contains methanol)