"Spectrophotometer"

Supplier: G-Biosciences

This lab activity involves analysis of an enzyme reaction using a specific enzyme substrate and inhibitor. Students study how the rate of enzyme reaction is dependent on substrate concentration and the influence of agents (inhibitors) and conditions that affects the enzyme reaction such as pH and temperature.

Supplier: Hach

Use the toxtrak™ toxicity reagent set to analyze your water or wastewater samples for toxicity.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ Pierce™ Rapid Gold BCA Protein Assay Kit is a two-component, high precision, detergent-compatible assay optimised to measure (at 480 nm) total protein concentration compared to a protein standard curve of known concentrations. The Pierce™ Rapid Gold BCA Protein Assay uses the same copper-chelating technology as the well-known traditional Pierce™ BCA Protein Assay and provides comparable accuracy but with a 5 minute, room temperature incubation and measured at 480 nm. This improvement eliminates the need to wait or expose the samples to elevated temperatures for a fast time to results.

Supplier: OZ BIOSCIENCES

SEAP assay kit (Secreted Alkaline Phosphatase) is a colorimetric assay for sensitive quantification of SEAP in culture medium from transfected cells or tissues.

Supplier: AAT Bioquest

Designed for consistent and dependable results. Portelite™ fluorimetric sodium ion quantification kit uses our robust sodium ion indicator dye, SoNa™ 520, which exhibit great fluorescence intensity enhancement upon binding to sodium ions.

Supplier: Thermo Orion

Orion™ AQUAfast tablet reagents perform colorimetric measurements of common water parameters with convenient, rapidly dissolving tablets.

Supplier: EDVOTEK

Most molecules have a net charge within a pH range of 2 to 10. When the pH is altered, the net charge on molecules can change drastically. In this experiment, a mixture of 2 chemicals is absorbed onto a solid support ion-exchange column and separated during elution under conditions that influence their net charge.

Supplier: AAT Bioquest

Horseradish peroxidase (HRP) and HRP conjugates facilitate the ABTS oxidation in the presence of hydrogen peroxide, turning ABTS into its blue-green oxidized product.

Supplier: Thermo Fisher Scientific

The Pierce™ Coomassie (Bradford) Protein Assay Kit is a ready-to-use, stable formulation of the traditional Bradford assay reagent to measure (A595 nm) total protein concentration compared to a protein standard.

Supplier: G-Biosciences

This lab activity is designed to demonstrate the different classes of protein molecules and their classification based on solubility. Students learn fractionation of soluble, insoluble membrane proteins, and cytoskeleton proteins from a tissue sample. The insoluble protein fraction is further fractionated into hydrophilic and hydrophobic membrane proteins. Cell membrane structure and the role of hydrophobic membrane proteins are considered. This lab activity also provides an opportunity to understand characteristics of various classes of detergents and the role of detergents in solubilisation of hydrophobic membrane proteins.

Supplier: EDVOTEK

In this experiment, students purify the restriction enzyme, Eco RI. This procedure utilised an ion exchange chromatograph step for Eco RI purification. Column fractions are assayed for the enzyme using Lambda DNA and digestion products are identified by gel electrophoresis. Fractions that contain Eco RI are identified and pooled. The total and specific activities are calculated. Recommended for advanced courses.

Supplier: Labcon

Ultra-clear polypropylene, graduated, autoclavable, and freezable microcentrifuge tubes with flat cap. Amber coloured styles are available for use with light-sensitive substances.

Supplier: Hach

This snapper is used for opening ampoules immersed in samples.

Supplier: Thermo Fisher Scientific

Yeast β-Galactosidase Assay Kit is specially formulated for yeast cells and ideal for identifying protein interactions in vivo using two-hybrid systems.

Supplier: Labcon

Ultra-clear polypropylene, graduated, autoclavable, and freezable microcentrifuge tubes with flat caps. Amber coloured styles are available for use with light-sensitive substances.

Supplier: Thermo Fisher Scientific

The Pierce™ Coomassie Plus Protein assay is a quick and ready-to-use, reducing agent-compatible, improved Bradford assay to quickly measure (A595 nm) total protein concentration compared to a protein standard.