2426 Ergebnisse für: "STEMCELL Technologies"

Supplier: STEMCELL Technologies

Hamster (Armenian) monoclonal IgG antibody against mouse CD11c, biotin-conjugated.

Supplier: STEMCELL Technologies

Rat monoclonal IgG2a antibody against mouse Sca1 (Ly-6A/E), biotin-conjugated.

Supplier: STEMCELL Technologies

Mouse monoclonal IgG1 antibody against rhesus red blood cells, unconjugated.

Supplier: STEMCELL Technologies

Rat monoclonal IgG2a antibody against human, mouse, rhesus CD49f, APC-conjugated.

Supplier: STEMCELL Technologies

Mouse monoclonal IgG1 antibody against human CD56 (NCAM), unconjugated.

Supplier: STEMCELL Technologies

Mouse monoclonal IgM antibody against human, mouse, rat oligodendrocyte marker O4, unconjugated.

Supplier: STEMCELL Technologies

Rat monoclonal IgG2b antibody against mouse CD4, PE-conjugated.

Supplier: STEMCELL Technologies

Rat monoclonal IgG2a antibody against mouse CD138 (syndecan-1), biotin-conjugated.

Supplier: STEMCELL Technologies

Rat monoclonal IgG2a antibody against human, mouse, cat CD45R (B220).

Supplier: STEMCELL Technologies

Rat monoclonal IgG2b antibody against human, mouse, rhesus CD11b, biotin-conjugated.

Supplier: STEMCELL Technologies

Hamster (Armenian) monoclonal IgG antibody against mouse CD11c, FITC-conjugated.

Supplier: STEMCELL Technologies

Rat monoclonal IgG2b antibody against mouse CD45, unconjugated.

Supplier: STEMCELL Technologies

Hamster (Armenian) monoclonal IgG1 antibody against mouse CD3e, FITC-conjugated.

Supplier: STEMCELL Technologies

Mouse monoclonal IgG2a antibody against human, rhesus, cynomolgus CD184 (CXCR4), PE-conjugated.

Supplier: STEMCELL Technologies

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD2, APC-conjugated.

Supplier: STEMCELL Technologies

Mouse monoclonal IgG1 antibody against human, chimpanzee, horse CD38, Alexa Fluor® 488-conjugated.

Supplier: STEMCELL Technologies

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD90 (Thy-1), PE-conjugated.

Supplier: STEMCELL Technologies

Interleukin 33 (IL-33) is a pro-inflammatory cytokine from the IL-1 family. It binds to ST2 receptor and activates NF-κB and MAPK pathways. IL-33 is expressed by epithelial cells, smooth muscle cells, and fibroblasts in various tissues and organs, as well as resting basophils, mast cells, eosinophils, natural helper cells, group 2 innate lymphoid cells, dendritic cells, and activated macrophages (Schmitz et al.; Yasuda et al.). It contributes to allergic inflammation by stimulating production of the cytokines IL-4, IL-5, and IL-13, and stimulates host defense against microbial and viral infections (Liew; Yasuda et al.). In the central nervous system, IL-33 is produced by endothelial cells and astrocytes. It induces proliferation of microglia and mediates production of pro-inflammatory cytokines (Yasuoka et al.).

Supplier: STEMCELL Technologies

Xeno-free and serum-free medium for culture and expansion of human hematopoietic cells.

Supplier: STEMCELL Technologies

Cell culture medium for establishment and maintenance of mouse pancreatic exocrine organoids.

Supplier: STEMCELL Technologies

Noggin binds to and antagonizes bone morphogenetic protein (BMP) ligands of the transforming growth factor beta (TGF-β) family. Noggin supports maintenance of undifferentiated human embryonic stem cells in vitro, and can be used to prevent spontaneous differentiation in the short term (Chaturvedi et al.). Noggin is essential for development of structures derived from ectoderm embryonic somite, skeletal patterning, and neurogenesis in vivo. It also influences chondrogenesis, osteogenesis, and joint formation (Krause et al.), and promotes dopaminergic differentiation of embryonic stem cells and subsequent survival of dopamine neurons (Chiba et al.).

Supplier: STEMCELL Technologies

Interleukin 12 (IL-12p70) is a heterodimeric cytokine composed of p35 and p40 subunits. IL-12 is produced by monocytes, macrophages, dendritic cells, neutrophils, and B cells in response to bacterial products and cytokines such as IFN-γ. The IL-12 receptor is expressed on T, NK, and dendritic cells. Upon binding, IL-12 initiates signaling via the JAK/STAT signaling pathway and stimulates NK, B, and T cells to produce IFN-γ (Watford et al.). It also regulates cytokine synthesis, proliferation of T and NK cells, and stimulates differentiation of CD4+ and CD8+ T cells (Germann and Rüde). Mice that are deficient in IL-12 are susceptible to many intracellular pathogens and have impaired IFN-γ secretion, Th1 differentiation, and NK cytolytic activity; however, Th2 development and IL-4 production are enhanced (Watford et al.).

Supplier: STEMCELL Technologies

Glial cell line-derived neurotrophic factor (GDNF) is a neurotrophic factor and a member of the tumor growth factor (TGF)-beta superfamily. The GDNF family of growth factors also includes neurturin, persephin, and artemin, which have seven conserved cysteine residues called cysteine-knots (Treanor et al.). GDNF family ligands signal through binding to specific GDNF-family receptor-α (GFRα) co-receptors and activate the RET receptor tyrosine kinase (Durbec et al.). Four different forms of GFRα co-receptors have been characterized (GFRα 1-4); GDNF binds specifically to GFRα1 prior to forming a complex with RET (Airaksinen and Saarma). GDNF is known to promote survival and morphological differentiation of midbrain dopaminergic neurons in both in vivo and in vitro studies and increases their high-affinity dopamine uptake (Granholm et al.; Lin et al.). GDNF has also been shown to have restorative effects on dying dopaminergic neurons in response to degenerative toxins (Aoi et al.). GDNF, together with Human Recombinant BDNF (brain-derived neurotrophic factor), BrainPhys™ Neuronal Medium, and other supplements, can be used to differentiate human pluripotent stem cell (hPSC)-derived neural progenitor cells into neurons (Bardy et al.).

Supplier: STEMCELL Technologies

Interleukin 17A (IL-17A) is the founding member of the family of cytokines that includes Interleukin 17B through Interleukin 17F. It is a potent proinflammatory cytokine that plays a key role in defense against pathogens. IL-17A and IL-17F signal as homodimers or heterodimers through the same receptor, and activate NF-kB, MAPK, and C/EBP pathways (Gaffen). IL-17A receptor is expressed on a variety of cell types, including hematopoietic cell compartments. IL-17A is produced by T helper 17 cells, CD8+ T cells, γδ T cells, natural killer T cells, B cells, neutrophils, innate lymphoid cells and mesenchymal stromal cells (MSCs; Zenobia and amp; Hajishengallis; Mojsilovic et al.). IL-17A receptor is expressed at particularly high levels on stromal cells, including MSCs. IL-17A increases the frequency and the average size of colony-forming units-fibroblast derived from bone marrow, as well as the proliferation of bone marrow-derived MSCs. IL-17A suppresses osteogenic differentiation and bone formation of bone marrow-derived MSCs. The action of IL-17A on hematopoiesis is deeply reliant on the microenvironment and the induction of other regulators. In healthy mouse bone marrow, IL-17A stimulates myeloid and early stage erythroid progenitor cells but inhibits late stage erythroid progenitor cells (Mojsilovic et al.).

Supplier: STEMCELL Technologies

Bone morphogenetic protein 4 (BMP-4) is a member of the highly conserved transforming growth factor β (TGF-β) superfamily. Mature BMP-4 is a disulfide-linked homodimeric protein consisting of two 116-amino-acid residue subunits, and is generated by the proteolytic removal of the signal peptide and propeptides (Xiao et al.). BMP-4 binds to type I and type II receptors on cells. This binding results in the phosphorylation of receptor 1, which in turn results in the phosphorylation of Smad proteins, which then go on to act as transcription factors (Zhang et al.). BMPs have been shown to be key regulators of embryogenesis and are known to play a role in the growth and differentiation of various cell types, including embryonic stem (ES) cells, induced pluripotent stem (iPS) cells, mesenchymal cells, epithelial cells, hematopoietic cells, and neuronal cells (Chadwick et al.; Graham et al.; Jones et al.; Lengerke et al.; Zhang et al.).

Supplier: STEMCELL Technologies

The SARS-CoV-2 (spike protein) peptide pool is provided as two lyophilised mixtures (subpools) from the SARS-CoV-2 spike protein. Each subpool contains 158 peptides, for a total of 316 peptides. The virus attaches to the cell membrane of the host through the interaction between spike protein and angiotensin-converting enzyme 2 (ACE2) receptor, and the spike protein plays a critical role in viral entry. The subpools consist of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 1273 on the spike protein.

Supplier: STEMCELL Technologies

cGMP, feeder-free maintenance medium for human ES and iPS cells.

Supplier: STEMCELL Technologies

Leukemia inhibitory factor (LIF) is an interleukin 6 class cytokine that regulates a broad variety of developmental functions. After LIF binds to the LIF receptor (LIFR), LIFR associates with gp130 and activates JAK/STAT and MAPK signaling (Auernhammer and Melmed; Suman et al.). LIFR activation of STAT3 is essential for maintaining the mouse embryonic stem cell phenotype (Niwa et al.). Produced by the endometrium, LIF plays an important autocrine and paracrine role in implantation by regulating proliferation, invasion, and differentiation of trophoblasts following blastocyst attachment (Auernhammer and Melmed; Suman et al.). Human LIF can be used for the maintenance of mouse embryonic stem cells; however, mouse LIF cannot bind to the human receptor, thus rendering mouse LIF inactive (Dahéron et al.). LIF is produced by CD4+ and activated regulatory T cells, and promotes Foxp3 expression, while repressing Th17 lineage-specific genes (Metcalfe). LIF is also secreted by mesenchymal stromal cells, where it supports hematopoiesis and immune modulation (Nasef et al.).

Supplier: STEMCELL Technologies

Dulbeccos phosphatgepufferte Kochsalzlösung mit 2 % fötalem Rinderserum wird als Puffer zum Verdünnen oder Waschen kernhaltiger Zellen empfohlen.

Supplier: STEMCELL Technologies

Cell dissociation reagent.