1057 Ergebnisse für: "SIGMA ALDRICH MICROSCOPY"

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Lumichrome (7,8-Dimethylalloxazine) is a natural metabolite of riboflavin. It is a flourescent photoproduct of riboflavin degradation. It is known to be an effective photosensitiser and a fluorescent dye. Lumichrome is suitable as a fluorescence probe, metal ion sensor, and may be used for the inactivation of biological contaminants. Its applications are also seen in memory and semiconductor devices.

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Sudan Red 7B has been used to stain the fat bodies of Ostrinia nubilalis (Hübner) larvae. Sudan red 7B, also known as fat red, is a non-fluorescent stain.[2] It is effectively used to stain lipids and is used to indicate the presence of suberin in cell walls.[2][3][4] Sudan red 7B acts as a marker for formosan subterranean termite, Coptotermes fornosanus Shiraki.

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Fluorescent protein label.

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Sodium 3,5-dichloro-2-hydroxybenzenesulfonate is used in conjunction with 4-aminoantipyrine (4-AAP) and hydrogen peroxide (H2O2) for chromogenic quantitation of uric acid in serum and urine samples.

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Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.

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3,3'-Diethyloxadicarbocyanine iodide (DODC) ≥99%, Sigma-Aldrich®

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Neutralrot, Sigma-Aldrich®

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1% hematoxylin in ethanol. To be used in conjunction with HT109.

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Wright′s Stain solution is commercially available and is generally used in hematology. Its preparation involves heating methylene blue with sodium bicarbonate and combining the mixture with eosin. It is alkaline, hence, its concentration is continuously changing due to the progressive oxidizing action of the alkali. Therefore, Giemsa′s stain is added to preserve the azurophilic properties of polychrome dyes.

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o-Dianisidine (3,3′-dimethoxybenzidine) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is yellow-orange in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 5 M HCl.

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Solvent Green 3, also known as Quinizarine Green SS (CI 61565) is an anthraquinone derivative. It is a green dye that imparts its colour to materials. Solvent Green 3 is made by condensing either leucoquinizarin or a mixture of leucoquinizarin and quinizarin with two moles of p-toluidine in the presence of boric acid.

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Tetranitroblau-Tetrazoliumchlorid, Sigma-Aldrich®

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Reagent for aluminium. Aurintricarboxylic acid readily polymerises in aqueous solution, forming a stable free radical that inhibits protein-nucleic acid interactions. It is a potent inhibitor of ribonuclease and topoisomerase II by preventing the binding of the nucleic acid to the enzyme. It stimulates tyrosine phosphorylation processes including the Jak2/STAT5 pathway in NB2 lymphoma cells, ErbB4 in neuroblastoma cells, and MAP kinases, Shc proteins, phosphatidylinositide 3-kinase and phospholipase C in PC12 cells. Aurintricarboxylic acid inhibits apoptosis in many cell types. Its neuroprotective effect, perhaps due to its ability to prevent down-regulation of Ca²⁺ impermeable GluR2 receptors or to its ability to inhibit calpain, a Ca²⁺-activated protease that is activated during apoptosis.

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Alizarin has been used as a biological stain to dye cotton fabrics and is used as standard in UV-visible spectrophotometer.

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Thymol is generally added in the Hematoxylin and Eosin staining solution to prevent mould growth. It is also suitable for use in antimicrobial screening by the disk diffusion method. It has been used as a test compound in antifungal assay to assess the susceptibility of various fungal isolates.

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Formalin solution, neutral buffered, 10% may be used as a reagent for the primary fixation of tissues for diagnostic applications of an immunoperoxidase method in hematopathology.

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Hanks' Balanced Salt solution,10 x, Modified, without calcium, magnesium or sodium bicarbonate

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Giemsa's Azur-Eosin-Methylenblau, Sigma-Aldrich®

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Useful for staining fresh, fixed, unembedded or plastic-embedded sections of bone. Villanueva osteochrome bone stain gives uniform and reproducible results. Useful in studying biopsy or postmortem tissue.

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Used in electrophoresis and as a hapten carrier. Most commonly used to prepare density gradients. A nonionic synthetic polymer of sucrose.

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Used to demonstrate polymorphonuclear cells in blood or bone marrow films. Each kit has sufficient reagents for 10 uses.

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For use with Sigma kit 86R, for the demonstration of alkaline phosphatase in granulocytes. The procedure is often referred to as the LAP procedure.

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Bouin's solution is an excellent fixative for preserving soft and delicate structures. It is prepared with saturated picric acid, formaldehyde and acetic acid.

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Nuclear counterstain. Included as a component in the Sigma Fetal Hemoglobin Procedure 285.

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Naphthol AS-phosphat ≥99% (durch TLC) für die Histologie, Sigma-Aldrich®

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Naphthol AS-MX phosphate disodium salt,phosphatase substrate

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Intended for use in Sigma Procedure 387 for the demonstration of Acid Phosphatase (TRAP).

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Fluoroshield™ with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

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Intended for use in the study of connective tissue, muscle and collagen fibers. Stains collagen blue and muscle red.

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Solvent Blue 59, Dye content 98 % (1,4-Bis(ethylamino)-9,10-anthraquinone; Atlasol Blue 2N; Sudan Blue)