"Corning"

Supplier: Corning

Penicillin-Streptomycin-L-Glutamin kombiniert zwei Antibiotika mit dem Nährstoff L-Glutamin, der unerlässlich ist für das Zellwachstum. Penicillin hemmt das Bakterienwachstum durch die Hemmung der Peptidoglycansynthese (unerlässlich für die bakteriellen Zellwand) und Streptomycin hemmt das Bakterienwachstum durch die Hemmung der Proteinsynthese. L-Glutamin ist von wesentlicher Bedeutung als Energieträger für die meisten Säugerzellen in der Kultur wegen seinem Aminosäuren Kohlenstoffskelett. Diese Antibiotika-Nährstoff-Kombination ist wirksam gegen Gram-negative und Gram-positive Bakterien.

Supplier: Corning

Kanamycinsulfat 5000 µg/ml

Supplier: Corning

Gentamycin wirkt als antimikrobieller Wirkstoff durch Bindung an die 30S-ribosomale Untereinheit von bestimmten Bakterien. Es enthält 50 mg/ml Gentamycin in deionisiertem Wasser und wird empfohlen für den Einsatz in Zellkulturanwendungen bei 1 ml/l. Dieses Produkt wirkt gegen Mycoplasmen, Gram-negative und Gram-positive Bakterien und ist bei 37 °C 5 Tage lang stabil.

Supplier: Corning

This is a combination of penicillin, streptomycin and Amphotericin formulated to contain 10000 units/ml penicillin G, 10 mg/ml streptomycin sulphate and 25 µg/ml amphotericin B.

Supplier: Corning

This antimycotic interferes with fungal membrane permeability by forming channels in cell membranes, causing small molecules to leak from the cell. It is recommended for use in cell culture applications at 10 ml/L and is stable at 37 ºC for 3 days.

Supplier: Corning

This product is a mix of the antibiotics Penicillin (10,000 IU/mL) and Streptomycin (10,000 μg/ml) in a 100-fold working concentration. Penicillin (Penicillin G) works by inhibiting peptidoglycan synthesis, while Streptomycin inhibits protein synthesis. Penicillin-Streptomycin is effective against Gram-negative and Gram-positive bacteria.

Supplier: Corning

This product is a mix of the antibiotics Penicillin (5,000 IU) and Streptomycin (5,000 μg/ mL) in a 50-fold working concentration. Penicillin (Penicillin G) works by inhibiting peptidoglycan synthesis, while Streptomycin inhibits protein synthesis. Penicillin-Streptomycin is effective against Gram-negative and Gram-positive bacteria. This antibiotic is recommended for use in cell culture media at 20 ml/L.

Supplier: Corning

Dimethyl Sulfoxide (DMSO) is commonly used in cell cryopreservation, along with glycerol, to prevent damage to the cell membrane during freezing.

Supplier: Corning

Animal free formulation

Supplier: Corning

Corning® cellgro® COMPLETE formulation is based on a combination of 1:1 DMEM/F12, RPMI, and McCoy's 5A medium. This medium contains a proprietary mixture of selected trace elements and high molecular weight carbohydrates, extra vitamins, animal origin-free protein, and a small amount of high quality bovine serum albumin (1,0g/L). The Corning cellgro COMPLETE supports superior growth and viability over long-term passages in hybridomas, along with suspensions and adherent cell cultures.

Supplier: Corning

Trypsin is used to enzymatically release adherent cells from tissue culture plates for passaging

Supplier: Corning

Trypan blue is the most common stain used in cell count and viability assays.

Supplier: Corning

Phosphate Buffered Saline (PBS) is used to maintain a physiological pH and osmolarity, two key factors in tissue culture and molecular biology research

Supplier: Corning

IPTG (isopropylthiogalactoside) is an inducer of the lac operon in bacteria, which is frequently used in cloning as a component of a recombinant plasmid.

Supplier: Corning

X-Gal (5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside) is a substrate of b-galactosidase, a gene product of the lac operon in E. coli.

Supplier: Corning

Carbenicillin is a form of penicillin and is often used in place of ampicillin because it maintains stability after being autoclaved.