"Biotium"
Pluronic® F-127 20% in DMSO
Supplier: Biotium
Ready-to-use solution of Pluronic® F-127, a nonionic detergent useful for solubilizing hydrophobic dye molecules such as cell-permeable AM esters of Fura-2, Fluo-3, Indo-1, or Rhod-2 calcium indicators to facilitate cell loading.
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Propidiumiodid 1 mg/ml in Wasser
Supplier: Biotium
Propidium iodide (PI) is a membrane-impermeant nucleic acid intercalator. The dye is commonly used to selectively stain dead cells in a cell population and also used as a counterstain in multicolor fluorescent imaging.
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Nukleinsäuren-Gel-Färbemittel, GelRed™ und GelGreen™
Supplier: Biotium
GelRed und GelGreen sind fluoreszierende Nukleinsäuren-Gel-Färbemittel, die hohe Empfindlichkeit, niedrige Toxizität und außergewöhnliche Stabilität in sich vereinen.
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qPCR master mixes, Forget-Me-Not™ EvaGreen®
Supplier: Biotium
EvaGreen® Dye and high-performance dye-based qPCR master mixes containing EvaGreen® qPCR dye, Cheetah™ HotStart Taq Polymerase, and Forget-Me-Not™ tracking dye. Available with 2-colour tracking or pre-mixed with low or high ROX.
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PMA (Propidium monoazide) DNA/RNA Vergleichsfarbstoff
Supplier: Biotium
PMA (propidium monoazide) is a high-affinity photoreactive DNA binding dye invented by scientists at Biotium for viability PCR of bacteria and other organisms. The dye is weakly fluorescent by itself but becomes more fluorescent after binding to nucleic acids.
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Cell viability assay kits
Supplier: Biotium
MTT, XTT, and resazurin are reduced by mitochondrial metabolic activity to yield coloured or fluorescent products, and thus are useful for and assaying cell viability and quantitating cell number. MTT and XTT are reduced to coloured formazin salts that can be measured by absorbance. MTT generates an insoluble formazin salt, requiring cell lysis before the absorbance can be measured, while XTT does not require cell lysis for measurement. Resazurin is a non-fluorescent blue dye that is reduced to the pink fluorescent compound resorufin, which can be measured by fluorescence or absorbance.
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Fluorometric polymerase activity assay kit, EvaEZ™
Supplier: Biotium
EvaEZ™ Fluorometric Polymerase Activity Assay Kit provides an easy and accurate way to determine the activity of a nucleic acid polymerase without using radioisotopes.
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Fast probe master mixes
Supplier: Biotium
A qPCR master mix containing Cheetah™ Taq hotstart DNA polymerase, suitable for probe-based real-time PCR applications.
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Dimethylsulfoxid, wasserfrei ≥99,9%
Supplier: Biotium
Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions.
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PMA (Propidium monoazide) 20 mM in Wasser DNA/RNA Vergleichsfarbstoff
Supplier: Biotium
PMA (propidium monoazide) dye is a DNA modifier invented by scientists at Biotium. It is a photo-reactive dye that binds to dsDNA with high affinity. Upon photolysis with visible light, PMA dye covalently attaches itself to dsDNA.
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Wheat germ agglutinin (WGA) conjugates
Supplier: Biotium
A wheat germ agglutinin (WGA) lectin labelled with Biotium’s CF® Dyes or HRP. It is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine moieties of glycoproteins. As a result, WGA conjugates will label yeast bud scars and the cell membrane of gram-positive bacteria and mammalian cells.
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Fluorescence antifade mounting media and CoverGrip™ coverslip sealant, EverBrite™
Supplier: Biotium
EverBrite™ mounting media are anti-fade mountants for preserving fluorescence during microscopy. EverBrite™ mounting media prevent rapid photobleaching of fluorescein, rhodamine, and cyanine based dyes and are optimally formulated for use with Biotum’s CF™ dye conjugates. Unlike Vectashield® EverBrite™ mounting media is compatible with Cy® dyes such as Cy®2, Cy®3 and Cy®5. EverBrite™ mounting medium is a wet-set medium while EverBrite™ hardset mounting medium cures overnight to form a hard permanent seal with the coverslip, eliminating the need to seal coverslip edges with nail polish or other sealants. Both formulations are available with DAPI for blue fluorescent nuclear counterstaining without an additional staining step.
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Zellschaber
Supplier: Biotium
PE Einweg, steril. Mini-Zellschaber zum Ernten von Zellen oder Zelllysaten aus Mikrotiterplatten.
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Decontamination bags, activated charcoal
Supplier: Biotium
Activated charcoal retains ethidium bromide, GelRed™ and GelGreen™ efficiently in a convenient bag for decontamination of used gel staining solutions. Each bag has a total capacity of at least 10 mg ethidium bromide.
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Nukleinsäure-Farbstoffe, PAGE GelRed™
Supplier: Biotium
Farbstoffe speziell für DNA in Polyacrylamid-Gelen.
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Lipofuszin-Autofluoreszenz-Löscher, TrueBlack™
Supplier: Biotium
TrueBlack™ ist ein Reagenz zur Lipofuszin-Autofluoreszenzlöschung in Gewebeschnitten für die Immunfluoreszenzfärbung. Lipofuszin besteht aus stark autofluoreszierenden Granula von oxidierten Proteinen und Lipiden, die sich in den Lysosomen alternder Zellen anreichern. Lipofuszin-Granula fluoreszieren hell in allen Kanälen, die für die Fluoreszenzmikroskopie verwendet werden, und akkumulieren sich mit zunehmendem Alter in verschiedenen Zell- und Gewebetypen.
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