3D Clear tissue clearing reagents and buffers, Corning®
Supplier: Corning
In our Starter Kit we have combined 30 ml of Corning 3D Clear with the four buffer solutions required to process 3D tissue cultures for immunolabelling. All you need to do is add your immunolabels and nuclear stains. This kit comes with enough material to process three 96-well plates of 3D cell culture models.
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Immersion oil viscosity 150 cSt (lit.), Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Immersion Oil has a defined refractive index of about 1,516. It is important that the refractive index (nD) is about 1,5, similar to that of glass. This enables a homogeneous oil immersion to be achieved.
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Microcavity flask, Corning® Elplasia®
Supplier: Corning
Culture thousands of spheroids in a T-75 footprint.
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